Background: Motion capture systems are widely used to quantify human gait. Two-dimensional (2D) video systems are simple to use, easily accessible, and affordable. However, their performance as compared to other systems (i.e. three-dimensional (3D) gait analysis) is not well established. Objectives: This work provides a comprehensive review of design specifications and performance characteristics (validity and reliability) of two-dimensional motion capture systems. Study design: Systematic review. Methods: A systematic literature search was conducted in three databases from 1990 to 2019 and identified 30 research articles that met the inclusion/exclusion criteria. Results: Reliability of measurements of two-dimensional video motion capture was found to vary greatly from poor to excellent. Results relating to validity were also highly variable. Comparisons between the studies were challenging due to differences in protocols, instrumentation, parameters assessed, and analyses performed. Conclusions: Variability in performance could be attributed to study design, gait parameters being measured, and technical aspects. The latter includes camera specifications (i.e. resolution and frame rate), setup (i.e. camera position), and analysis software. Given the variability in performance, additional validation testing may be needed for specific applications involving clinical or research-based assessments, including specific patient populations, gait parameters, mobility tasks, and data collection protocols. Clinical relevance This review article provides guidance on the application of 2D video gait analysis in a clinical or research setting. While not suitable in all instances, 2D gait analysis has promise in specific applications. Recommendations are provided about the patient populations, gait parameters, mobility tasks, and data collection protocols.