Static magnetic field-enhanced osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells via matrix vesicle secretion

Chang, Ching Yi; Lew, Wei Zhen; Feng, Sheng; Wu, Chung Lung; Wang, Hsin-Hui; Hsieh, Sung Chih; Huang, Haw Ming

doi:10.1080/09553002.2020.1787545

Cited by 13 publications

(12 citation statements)

References 30 publications

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“…As the reports in the literature proposed that, the static magnetic field with magnetic particles could induce osteogenic differentiation (Maredziak et al, 2016, Chang et al, 2020). Here we propose a comparable result of two different magnetic strengths (Figure 6).…”

Section: Resultsmentioning

confidence: 95%

Magnetically stimulated cryogels to enhance osteogenic and chondrogenic differentiation of stem cells

Odabaş

Tevlek

Erenay

et al. 2021

Preprint

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Cells can respond to the physical stimulus that comes from their micro-environments. There are several strategies to alter cell behavior. Several tissues like bone and cartilage, which are the point of interest of regenerative medicine, are under significant degrees of mechanical stress in real life. Within this stress, the arising mechanotransduction effect may trigger several behavioral responses on cells. As a novel and efficient way, magnetic nanoparticles can be used to make such a mechanotransductive effect on cells. In this study, pre-functionalized Fe3O4 superparamagnetic magnetite nanoparticles were synthesized and used to fabricate gelatin-based magnetic cryogels. Cell growth, tissue-specific metabolic activities, differentiation potential to the bone, and cartilage under static magnetic field at different magnetic field strength (1000-4000G) were investigated. Results indicated that there was a better induction in considerable higher magnetic field among all others and magnetic cryogels helps to mediate mesenchymal stem cell behaviour, promote their growth and induce osteogenic and chondrogenic differentiation.

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Section: Resultsmentioning

confidence: 95%

Magnetically stimulated cryogels to enhance osteogenic and chondrogenic differentiation of stem cells

Odabaş

Tevlek

Erenay

et al. 2021

Preprint

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“…This could be associated to the release of MtVs by the cells in culture. A recent in vitro study showed that static magnetic fields enhanced the osteogenic differentiation of MSCs and increased mineral formation through the release of MtVs into the extracellular matrix [195][196][197].…”

Section: Physical Stimuli For Mtv Secretionmentioning

confidence: 99%

Matrix Vesicles: Role in Bone Mineralization and Potential Use as Therapeutics

et al. 2021

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Bone is a complex organ maintained by three main cell types: osteoblasts, osteoclasts, and osteocytes. During bone formation, osteoblasts deposit a mineralized organic matrix. Evidence shows that bone cells release extracellular vesicles (EVs): nano-sized bilayer vesicles, which are involved in intercellular communication by delivering their cargoes through protein–ligand interactions or fusion to the plasma membrane of the recipient cell. Osteoblasts shed a subset of EVs known as matrix vesicles (MtVs), which contain phosphatases, calcium, and inorganic phosphate. These vesicles are believed to have a major role in matrix mineralization, and they feature bone-targeting and osteo-inductive properties. Understanding their contribution in bone formation and mineralization could help to target bone pathologies or bone regeneration using novel approaches such as stimulating MtV secretion in vivo, or the administration of in vitro or biomimetically produced MtVs. This review attempts to discuss the role of MtVs in biomineralization and their potential application for bone pathologies and bone regeneration.

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“…However, cells showed enhanced differentiation toward mature osteoblasts through increasing ALPasespecific activity. Extracellular matrice vesicles derived from plasma membranes were found around the stimulated cells when observed under a transmission electron microscope [24] and scanning electron microscope [59]. These so-called microvesicles are believed to promote mineral deposition and angiogenesis [12][13][14].…”

Section: Smfs Regulate Cell Fate For Gbr and Gtrmentioning

confidence: 99%

The Review of Bioeffects of Static Magnetic Fields on the Oral Tissue-Derived Cells and Its Application in Regenerative Medicine

Lew

Feng

Lee

et al. 2021

Cells

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Magnets have been widely used in dentistry for orthodontic tooth movement and denture retention. Nevertheless, criticisms have arisen regarding the biosafety of static magnetic field (SMF) effects on surrounding tissues. Various controversial pieces of evidence have been discussed regarding SMFs on cellular biophysics, but little consensus has been reached, especially in the field of dentistry. Thus, the present paper will first review the safe use of SMFs in the oral cavity and as an additive therapy to orthodontic tooth movement and periodontium regeneration. Then, studies regarding SMF-incorporated implants are reviewed to investigate the advantageous effects of SMFs on osseointegration and the underlying mechanisms. Finally, a review of current developments in dentistry surrounding the combination of magnetic nanoparticles (MNPs) and SMFs is made to clarify potential future clinical applications.

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Static magnetic field-enhanced osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells via matrix vesicle secretion

Cited by 13 publications

References 30 publications

Magnetically stimulated cryogels to enhance osteogenic and chondrogenic differentiation of stem cells

Magnetically stimulated cryogels to enhance osteogenic and chondrogenic differentiation of stem cells

Matrix Vesicles: Role in Bone Mineralization and Potential Use as Therapeutics

The Review of Bioeffects of Static Magnetic Fields on the Oral Tissue-Derived Cells and Its Application in Regenerative Medicine

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