This
study is a bioinformatics assay on the microbial genome of
Bacteroides thetaiotaomicron
. The study focuses on
the problem of quorum sensing as a result of adverse factors such
as chemotherapy and antibiotic therapy. In patients with severe intestinal
diseases, two strains of microorganisms were identified that were
distinguished as new. Strains were investigated by conducting genome
sequencing. The current concepts concerned with the quorum sensing
system regulation by stationary-phase sigma factor and their coregulation
of target genes in
B. thetaiotaomicron
were considered. The study suggested using bioinformatics data for
the diagnosis of gastrointestinal disorders. In the course of the
study, 402 genes having a greater than twofold change were identified
with the 95% confidence level. The shortest and longest coding genes
were predicted; the noncoding genes were detected. Biological pathways
(KEGG pathways) were classified into the following categories: cellular
processes, environmental information processing, genetic information
processing, human disease, metabolism, and organismic systems. Among
notable changes in the biofilm population observed in parallel to
the planktonic
B. thetaiotaomicron
was
the expression of genes in the polysaccharide utilization loci that
were involved in the synthesis of O-glycans.