Statistical analysis of data for three British ethnic groups from a new STR multiplex

Evett, I.W.; Gill, Peter; Lambert, J.A.; Oldroyd, Nicola J.; Frazier, R.; Watson, Steve P.; Panchal, Sasikala; Connolly, Alexandra; Kimpton, C.

doi:10.1007/bf02441017

Cited by 54 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Allele frequency data generated by the ParaDNA Intelligence Test (Table 3) indicated a similar distribution of alleles to those measured using the AmpFlSTR SGM Plus [5,6]. The multi-locus probability of identity (system PI), calculated by multiplying the individual PI values of each locus, is often used as a measure of the discriminatory ability of a profiling system [18].…”

Section: Resultsmentioning

confidence: 80%

“…One reason for discordance between the ParaDNA Intelligence Test and AmpFlSTR SGM Plus is the occurrence of microvariants. The most common microvariant, the 9.3 variant in TH01 [4][5][6], is correctly called as 9.3+ by the ParaDNA Intelligence Test. However due to the lower resolution of melting curve analysis compared with capillary electrophoresis (CE), other microvariants may not be distinguished from full repeat variants by the Para DNA Intelligence Test.…”

Section: Discussionmentioning

confidence: 99%

“…Discounting the occurrence of null alleles, the allele range at each locus detected and reported by the ParaDNA Intelligence Test is anticipated to cover 94.13% expected alleles at these loci, based on allele frequencies from sample populations [4][5][6]. A further 5.76% more rare alleles with repeat numbers above these ranges are also detected but cannot be resolved.…”

Section: Introductionmentioning

confidence: 99%

“…Concordance studies are typically conducted on a number (typically several hundred or more) of samples from individuals in a reference population or population subgroup using one profiling kit for which profiling data has been obtained using another product, thus establishing concordance between the profiles generated by the two products [4,10,11]. In addition, population studies can provide data on allele frequencies anticipated in the reference population or populations [5,6,12]. This report describes the high level of concordance demonstrated when DNA samples from 381 UK Caucasian individuals were profiled using the ParaDNA Intelligence Test compared with AmpFlSTR SGM Plus.…”

Section: Introductionmentioning

confidence: 99%

“…This report describes the high level of concordance demonstrated when DNA samples from 381 UK Caucasian individuals were profiled using the ParaDNA Intelligence Test compared with AmpFlSTR SGM Plus. This population was chosen as a relevant, well characterised population group upon which to conduct this concordance study [5,6]. Similar studies have sampled similar numbers of individuals from single [13,14] or multiple ethnic groups [4,8,9] represented within their geographical location.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Concordance study between the ParaDNA ® Intelligence Test, a Rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR ® SGM Plus ® )

Ball

Dawnay

Stafford-Allen

et al. 2015

Forensic Science International: Genetics

View full text Add to dashboard Cite

The ParaDNA® Intelligence Test enables STR profiling directly from human biological samples and evidence items collected from crime scene in 75 min. Designed for non-expert use this system allows DNA information to be available to investigators before it would typically be available from a laboratory. The ParaDNA Intelligence Test system amplifies D3S1358, D8S119, D16S539, D18S1358and TH01 STR loci and the gender typing locus amelogenin and detects the alleles present with HyBeacon1 probes. Individual DNA samples from 381 UK Caucasian individuals were analysed using AmpFlSTR1 SGM Plus® and the ParaDNA Intelligence Test with the derived STR profiles compared. Here we describe the high level of concordance demonstrated between the two systems and discuss this with reference to allele frequencies and the discriminatory power offered by the ParaDNA Intelligence Test.

show abstract

Section: Resultsmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Concordance study between the ParaDNA ® Intelligence Test, a Rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR ® SGM Plus ® )

Ball

Dawnay

Stafford-Allen

et al. 2015

Forensic Science International: Genetics

View full text Add to dashboard Cite

show abstract

Microsatellite variation in Central Africa: An analysis of intrapopulational and interpopulational genetic diversity

Destro-Bisol

Boschi

Caglià

et al. 2000

Am. J. Phys. Anthropol.

View full text Add to dashboard Cite

As a part of a research project on molecular variation in Central Africa, we have analyzed 10 microsatellites (CD4, CSFO, D3S1358, D18S51, D21S11, F13A1, FES, TH01, TPOX, and VWA) in the Bamileke and Ewondo from Cameroon and the Sanga and Mbenzele Pygmies from the Central African Republic (a total of 390 chromosomes). A statistically significant trend towards heterozygote deficiency was detected in the Mbenzele Pygmies. This was established through the use of powerful exact tests for the Hardy-Weinberg equilibrium. A certain degree of isolation and a small effective size may explain this finding. However, the lack of any substantial reduction in allelic diversity in the Mbenzele does not support the possibility that this group has a smaller effective size in evolutionary terms. A possible explanation based on ethnographic studies suggests that the gene flow from non-Pygmies to Pygmies could have been interrupted only in relatively recent times. The analysis of association between genotypes at pairs of independent loci indicates that the level of subheterogeneity is markedly lower in the Bamileke than in other sampled populations. This may be explained by the combined effect of larger population size, more rigid respect of clanic exogamy, and higher matrimonial mobility of the Bamileke. Finally, we have analyzed interpopulational relationships among our sampled populations and other Central African populations. The results are consistent with a previous study of protein loci (Spedini et al. 1999), which suggests the recent history of the Bamileke and Ewondo has led them to aquire a substantial genetic similarity. Furthermore, the Mbenzele Pygmies diverge from Biaka Pygmies, despite their common origin and geographical proximity. This is probably due to the differentiating effect of genetic drift, which is enhanced by the small effective size of Pygmy populations.

show abstract

DNA: an Overview

Graham

2009

Wiley Encyclopedia of Forensic Science

View full text Add to dashboard Cite

Forensic DNA profiling has gone through several transformations since its initial conception, to become the flexible and powerful technique that is used world wide. By exonerating the innocent, implicating the guilty, identifying the deceased, and resolving paternity disputes, DNA profiling has forced its way to the forefront of public consciousness to become an almost ubiquitous part of criminal and civil investigations where the identity of key players is of importance. This article charts the history of DNA profiling, from the “Eureka” moment of Prof Sir Alec Jefferies to contemporary technologies before introducing the key laboratory techniques required to process biological samples from DNA extraction to profile production and interpretation.

show abstract

Statistical analysis of data for three British ethnic groups from a new STR multiplex

Cited by 54 publications

References 20 publications

Concordance study between the ParaDNA ® Intelligence Test, a Rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR ® SGM Plus ® )

Concordance study between the ParaDNA ® Intelligence Test, a Rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR ® SGM Plus ® )

Microsatellite variation in Central Africa: An analysis of intrapopulational and interpopulational genetic diversity

DNA: an Overview

Contact Info

Product

Resources

About