Statistical Approach for Production of PUFA fromKocuriasp. BRI 35 Isolated from Marine Water Sample

Abstract

In this study, Plackett-Burman design was used to identify the most influential parameters affecting PUFA production by Kocuria sp. BRI 35 isolated from Antarctic water sample. Amongst 10 variables evaluated, magnesium chloride, protease peptone, glucose, and temperature were significant. Response surface methodology consisting of a central composite design was developed to study the interactions between the variables and to determine optimal values of significant variables. A quadratic model (R = 0.9652, F = 14.64, P < 0.0001) was built. The contour plots indicated that the isolate produced maximum PUFA at lower concentrations of magnesium sulfate (0.9 g/L) and higher concentrations of protease peptone (5 g/L) and glucose (10 g/L) at 15°C. MgSO4 and glucose exhibited quadratic as well as interactive effect on PUFA production whereas protease peptone and temperature showed interactive effects only. After optimization, PUFA production per unit biomass increased from 0.94 mg/g to 11.12 mg/g. This represented an increase from 3% to 58.62% of the total fatty acids. Among PUFAs, the yield of ω-6 fatty acids increased from 9.66 mg/L to 107.71 mg/L with significant increase in linoleic acid (20.36 mg/L) whereas ω-3 fatty acids increased up to 12.37 mg/L with DHA being the major ω-3 fatty acid produced.

Marine Oomycetes (<i>Halophytophthora</i> and <i>Salispina</i>): A Potential Source of Fatty Acids with Cytotoxic Activity Against Breast Adenocarcinoma Cells (MCF7)

“…The isolation of fatty acids was adapted from Tilay and Annapure 21 and Pote and Bhadekar 22 . Mycelial mass was freeze-dried, homogenized, and soaked in ethyl acetate/ methanol solution 2:1 v/v for 24 h. The suspension was collected, concentrated using a rotary evaporator at 40 , saponified using 0.5 N sodium hydroxide or was esterified using 0.5 N methanolic sodium hydroxide solution and heated at 90 for 15 min.…”

“…The hydrolysate was used for toxicity analysis and the esterified sample was used for the fatty acid composition analysis using gas chromatography. Esterified or FAME Fatty Acid Methyl Esters samples were analyzed using gas chromatography GC following several published protocols 2,3,21,22 . Samples were filtered into a sample vial using Whatman No.1 and filtrates were placed in an autosampler injector.…”

Isolation of Gram‐positiveFirmibacteriaas major eicosapentaenoic acid producers from subtropical marine sediments

“…as PUFA producers (Yazawa, ; Cho and Mo, ; Bianchi et al, ). Only Gram‐positive bacteria reported from Antarctic waters was Kocuria marina which produced both EPA and DHA (Pote and Bhadekar, ). In an attempt to isolate PUFA producers from tropical regions, Patnayak and Sree, () reported the isolation of one EPA producing Bacillus subtilis from sponge‐associated bacteria from Bay of Bengal of Indian Ocean.…”

Anti-microfouling Activity of Glycomyces sediminimaris UTMC 2460 on Dominant Fouling Bacteria of Iran Marine Habitats

“…The composition of bacterial biofilm communities during the first stage of biofouling period, not only differs based on the seawater in which the substrata of the biofilms were submerged but also depends on the time course and surface type. Despite the isolation of Kocuria members from different marine sources Dastager et al (2014) and Pote and Bhadekar (2014), there hasn’t been any reports demonstrating the biofilm forming abilities of this genus rather than their degradation activity. Hence, this result can trigger further studies in the field of biofilm formation and quorum sensing investigations of this genus.…”