Statistical medium optimization for the production of collagenolytic protease by Pseudomonas sp. SUK using response surface methodology

Bhagwat, Prashant; Jhample, Sowmya B.; Dandge, Padma B.

doi:10.1134/s0026261715040037

Cited by 27 publications

(16 citation statements)

References 30 publications

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“…They provide a crucial contribution for understanding the interactions between the two variables and finding their optimum levels (Jhample et al 2015). The circular order of counter plots indicates that the mutual interactions among corresponding variables are non-significant, while the elliptical order specifies significant interactions between corresponding variables (Bhagwat et al 2015). Two interacting variables were kept at optimum levels and the remaining variables were kept at zero level.…”

Section: Resultsmentioning

confidence: 99%

Response surface methodology-based optimization of production media and purification of α-galactosidase in solid-state fermentation by Fusarium moniliforme NCIM 1099

2016

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Response surface methodology was used to enhance the production of α-galactosidase from Fusarium moniliforme NCIM 1099 in solid-state fermentation. Plackett–Burman design was employed for selection of critical media constituents which were optimized by central composite rotatable design. Wheat bran, peptone and FeSO4·7H2O were identified as significant medium components using PB design. Further CCRD optimized medium components as wheat bran; 4.62 μg, peptone; 315.42 μg, FeSO4·7H2O; 9.04 μg. RSM methodological optimization increased the enzyme production from 13.17 to 207.33 U/g showing 15.74-fold enhancement. The α-galactosidase was purified by 70% fractionation followed by DEAE anion exchange column chromatography which yields 23.33% with 28.68-fold purification. The molecular weight of α-galactosidase was 57 kDa which was determined by SDS-PAGE analysis. Purified enzyme has optimum pH of 4.0 and was found to be stable in wide pH range of 3.0–9.0. Its optimum temperature was 50 °C, whereas its activity remains above 50% up to 2 h at 75 °C. Hg2+ was found to be a potent inhibitor and Mg2+ acted as an activator of enzyme. No significant change was observed in enzyme activity for galactose concentration, ranging from 1 to 100 mM. The K m values of enzyme for substrates p-nitrophenyl-α-d-galactopyranoside, melibiose and raffinose were 0.20, 1.36, and 3.66 mM, respectively. Low K m and stability to various physiological conditions of enzyme represents its potential which can be exploited in various industrial applications.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0575-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Response surface methodology-based optimization of production media and purification of α-galactosidase in solid-state fermentation by Fusarium moniliforme NCIM 1099

2016

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“…Therefore after extraction of collagen by acid hydrolysis treatment from scales, same scales were again treated with partially purified collagenolytic protease of Pseudomonas strain SUK which was previously isolated in our lab (Bhagwat et al, 2015). 10% scales were autoclaved and strain SUK enzyme (50 U) was added in the test.…”

Section: Enzyme Aided Recovery and Degradation Of Collagen From Scalesmentioning

confidence: 99%

Isolation, characterization and valorizable applications of fish scale collagen in food and agriculture industries

Bhagwat

Dandge

2016

Biocatalysis and Agricultural Biotechnology

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“…Bacteriocin production from Lactobacillusviridescence (NCIM 2167) is novel source which has not been investigated so far. (Drapeau et al 1972;Brunder et al 1997;Anwar and Saleemuddin 1998;Thangam and Rajkumar 2002;Bhagwat et al 2015).Thus it clearly shows that only few proteases could break down the bacteriocin as per their specificity towards respective bacteriocin. Amongst Escherichia coli showed the better results, hence it is used for further studies.Bacteriocin from culture supernatant showed the total activity of 6.2×10 8 AU/mL (Table2).…”

Section: Resultsmentioning

confidence: 98%

Production and Characterization of Bacteriocin Produced by Lactobacillus Viridescence(NICM 2167)

Bhagwat

et al. 2016

Braz. arch. biol. technol.

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Statistical medium optimization for the production of collagenolytic protease by Pseudomonas sp. SUK using response surface methodology

Cited by 27 publications

References 30 publications

Response surface methodology-based optimization of production media and purification of α-galactosidase in solid-state fermentation by Fusarium moniliforme NCIM 1099

Response surface methodology-based optimization of production media and purification of α-galactosidase in solid-state fermentation by Fusarium moniliforme NCIM 1099

Isolation, characterization and valorizable applications of fish scale collagen in food and agriculture industries

Production and Characterization of Bacteriocin Produced by Lactobacillus Viridescence(NICM 2167)

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