Statistical Optimization for the Development of a Culture Medium Based on the Juice of Waste-Dates for Growth of Lactococcus lactis LCL Strain by Using the Plackett–Burman and Response Surface Methodology

Gherab, Fatma Zohra Ras El; Hassaïne, Omar; Zadi‐Karam, Halima; Karam, Nicole

doi:10.1007/s12649-018-0283-0

Cited by 6 publications

(6 citation statements)

References 45 publications

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“…This work was carried out on a new culture medium based on date juice which has been described by [17], using the Lactococcus lactis DBH10 strain being obviously the most productive of lactic acid. This ability to acidification, is a character often reported as being be dependent of the isolate linked to the particular abilities that the latter has to effectively use the compounds of the culture medium and thus to make it more assimilable [22].…”

Section: Resultsmentioning

confidence: 99%

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Statistical Optimization of Lactic Acid Production by the Lactococcus lactis DBH10 Strain in a New Culture Medium Based on Date Waste (DJM), Using Response Surface Methodology

Midoun¹,

Hassaïne²

2023

actamanil

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We have successfully applied in this work the response surface methodology (RSM) involving central composite planes (CCD) in order to study the effect of the temperature of incubation (x 1 ), pH (x 2 ) and Inoculum size (x 3 ) on lactic acid production by the Lactococcus lactis DBH10 strain. After statistical analysis, the quadratic terms of the variable (x 1 2 , x 2 2 , x 3 2 ) as well as those of the variables (x 2 , x 3 ) in linear terms and their interactions (x 1 x 2 ; x 1 x 3 ) were found to have significant effects on the response of interest. The analysis of the second order polynomial regression model obtained by multiple regression analysis, allowed us to reach a maximum lactic acid concentration of 486 ± 2.4 °D. The mathematical relationship of lactic acid production by Lactococcus lactis DBH10 strain related to the three significant independent variables was performed by a satisfactory fitting model. The predicted values were found to be in good agreement with the experimental values, displaying a coefficient of determination of R2 = 0.989, demonstrating good significance of the proposed model. The adjustment of the levels of the factors predicted by the model resulted in a maximum lactic acid concentration, when the optimal combination of inculum size, temperature, and pH, are respectively at 2.8% (v/v), 21.5 °C and 4.8. The Validation of the predicted model was carried out by performing three more experiments at the optimal levels of these variables, thus confirming its robustness.

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Section: Resultsmentioning

confidence: 99%

“…Date Juice Medium (DJM) preparation. The date's juice preparation was performed according to the method described by [17]. The substrate was obtained from ripe dates fruits with low commercial value.…”

Section: Methodsmentioning

confidence: 99%

Statistical Optimization of Lactic Acid Production by the Lactococcus lactis DBH10 Strain in a New Culture Medium Based on Date Waste (DJM), Using Response Surface Methodology

Midoun¹,

Hassaïne²

2023

actamanil

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“…Lactobacillus is capable of assimilating fructose for glycolysis or converting it to mannitol through the activity of mannitol dehydrogenase. During the later stage of fermentation, acetic acid bacteria metabolize gluconic and acetic acids under aerobic conditions to enhance the acidic flavor (Lynch et al, 2021;Ras El Gherab et al, 2019). The final pH of the drink was noted to be below 4, representing a crucial feature of the beverage's safety.…”

Section: Effects Of the Variables On Ph Ta And Sscmentioning

confidence: 99%

Process optimization of a novel water kefir drink with high antioxidant activity from Chinese jujube (Ziziphus Jujuba Mill.) Through response surface methodology

Xu,

Shu,

Dai

et al. 2023

Acta Sci Pol Technol Aliment

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“…PBD offers an efficient route to optimize large number of variables and identifies the most significant variables. The design has been successfully applied in medium optimization by various previous studies [44][45][46][47]. CCD was employed to optimize the concentrations of these important three variables for maximum L-LA production.…”

Section: L-la Production From Mixed Glucose and Xylose By Coculture Fermentationmentioning

confidence: 99%

Improvement of Enantiomeric l-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of Enterococcus mundtii WX1 and Lactobacillus rhamnosus SCJ9

et al. 2021

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Among 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure l-lactic acid (l-LA) from glucose, xylose and arabinose with furfural-tolerant properties. The isolate WX1 was identified as Enterococcus mundtii based on 16S rDNA sequence analysis. The conversion yields of l-LA from glucose and xylose by E. mundtii WX1 were 0.97 and 0.68 g/g substrate, respectively. Furthermore, l-LA production by E. mundtii WX1 in various glucose-xylose mixtures indicated glucose repression effect on xylose consumption. The coculture of E. mundtii WX1 and Lactobacillus rhamnosus SCJ9, a homofermentative LAB capable of producing l-LA from glucose clearly showed an improvement of l-LA production from 30 g/L total glucose-xylose (6:4). The results from Plackett–Burman design (PBD) indicated that Tween 80, MnSO4 and yeast extract (YE) were three medium components that significantly influenced (p < 0.05) l-LA production using the coculture strategy in the presence of 2 g/L furfural. Optimal concentrations of these variables revealed by central composite design (CCD) and response surface methodology (RSM) were 20.61 g/L YE, 1.44 g/L Tween 80 and 1.27 g/L MnSO4. Based on the optimized medium with 30 g/L total glucose-xylose (6:4), the maximum experimental l-LA value of 23.59 g/L reflecting 0.76 g/g substrate were achieved from 48 h fermentation at 37 °C. l-LA produced by coculture cultivated under standard MRS medium and new optimized conditions were 1.28 and 1.53 times higher than that obtained from single culture by E. mundtii WX1, respectively. This study provides the foundations for practical applications of coculture in bioconversion of lignocellulose particularly glucose-xylose-rich corn stover to l-LA.

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Statistical Optimization for the Development of a Culture Medium Based on the Juice of Waste-Dates for Growth of Lactococcus lactis LCL Strain by Using the Plackett–Burman and Response Surface Methodology

Cited by 6 publications

References 45 publications

Statistical Optimization of Lactic Acid Production by the Lactococcus lactis DBH10 Strain in a New Culture Medium Based on Date Waste (DJM), Using Response Surface Methodology

Statistical Optimization of Lactic Acid Production by the Lactococcus lactis DBH10 Strain in a New Culture Medium Based on Date Waste (DJM), Using Response Surface Methodology

Process optimization of a novel water kefir drink with high antioxidant activity from Chinese jujube (Ziziphus Jujuba Mill.) Through response surface methodology

Improvement of Enantiomeric l-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of Enterococcus mundtii WX1 and Lactobacillus rhamnosus SCJ9

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