Statistical Optimization of Process Parameters for Bioplastic (PHA) Production by Bacillus subtilis NCDC0671 Using Orange Peel-Based Medium

Umesh, Mridul; Mani, Vellingiri Manon; Thazeem, Basheer; Preethi, Kathirvel

doi:10.1007/s40995-017-0457-9

Cited by 29 publications

(16 citation statements)

References 24 publications

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“…The process was run at optimum conditions of pH 7.3, temperature 37 °C , inoculums size 3% and incubation time 72 h in liquid medium containing 4% glucose, 3.5% peptone, 0.3% K 2 HPO 4 , 0.1% KH 2 PO 4 , 0.05% MgSO 4 .7H 2 O, and 0.03% l-cysteine [3]. Similarly, Umesh et al [9] obtained highest yield of 5.09 g/ L through submerged fermentation with Bacillus subtilis NCDC0671 with the optimized medium consisting of 2.23 (g/L) beef extract, 0.72 (g/L) sodium chloride and 48 h of incubation period. Nisha et al, [13] reported PHB yield of 2.2g/L by culturing Bacillus sphaericus NCIM 5149 through submerged fermentation.…”

Section: Results and Discussion Face Centred Central Composite Designmentioning

confidence: 99%

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Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Reddy¹,

Prabhakar²,

Venkateswarulu³

et al. 2020

CTBP

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Eco-friendly biopolymers, polyhydroxybutyrate, have been produced by many kinds of bacteria that possess most important applications in food packaging industries and also in medical field. In the present work, PHB production was economized through the statistical optimization of physical variables. The physical variables chosen for the enhanced production of PHB were incubation period, temperature, pH and inoculums size. The relative rate of production has been studied by understanding the complex interactions of variables using face-centred central composite design. The submerged fermentation with Acinetobacter nosocomialis RR20 produced PHB yield of 5.88 g/L at optimized conditions with a notable value change of sixfold increase in comparison with minimal salt medium and these findings have showed that the designed medium was significant in terms of higher of PHB production.

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Section: Results and Discussion Face Centred Central Composite Designmentioning

confidence: 99%

“…PHB assay Extraction and Quantification of PHB : Extraction of PHB from fermented broth was done as per the method of Umesh et al, [9] using chloroform. Acinetobacter nosocomialis RR20 was grown in the culture medium .The cells were harvested by centrifugation at 10,000g for 10 min.…”

Section: Shake Flask Fermentationmentioning

confidence: 99%

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Reddy¹,

Prabhakar²,

Venkateswarulu³

et al. 2020

CTBP

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“…Studies have used PBD and BBD for optimizing variables for higher PHA yield from microorganisms such as Acinetobacter junii , [ 126 ] and Bacillus subtilis NCDC0671. [ 127 ] For the production of PHA from A. Junii , three significant factors were selected, that is, glycerol, KH 2 PO 4 , and incubation time by PBD and BBD. PHA yield before optimization was 0.52 ± 0.05 g L −1 for 1.07 ± 0.32 g L −1 CDW which after optimization increased up to 3.04 g L −1 PHA.…”

Section: Media Optimization Strategies and Limitationsmentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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“…The mixture was further centrifuged at 5000 rpm for 15 min and washed with distilled phosphate buffer saline, water, acetone and methanol respectively. After washing, the pellet was dissolved in 5 ml of boiling chloroform and kept for complete evaporation to obtain PHB crystal [24].…”

Section: Phb Extraction From Bacterial Isolatesmentioning

confidence: 99%

Screening and Biochemical Characterization of PHB Producing Bacterium Isolated from Costal Region of Andhra Pradesh

Aluru¹

2020

EESRJ

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Biopolymers and their composites have been massively investigated in recent years for multiple applications especially in environmental medical and pharmaceutical fields. A thermoplastic that is both biodegradable and environmental friendly, as well as biocompatible is PHB. PHB is an intracellular granule, which is a carbon and energy reservoir for the bacteria under starvation or stress conditions. In the present work, we focused on identification of potential PHB (Polyhydroxybutyrate) producing bacterial strains from sewage soil of coastal region of Andhra Pradesh. Five bacterial isolates were identified through Sudan Black staining, out of which RR02 was observed to be potential for PHB production. Further, PHB extraction was performed by solvent extraction method. The extracted sample was characterized by FTIR, melting temperature Tm, was determined by DSC and then, compared with standard PHB for confirmation of quality. Biochemical characterization was also performed for preliminary identification of the bacterial isolate. Based on the study it was found to be Bacillus sp.

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Statistical Optimization of Process Parameters for Bioplastic (PHA) Production by Bacillus subtilis NCDC0671 Using Orange Peel-Based Medium

Cited by 29 publications

References 24 publications

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Screening and Biochemical Characterization of PHB Producing Bacterium Isolated from Costal Region of Andhra Pradesh

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