Status of benzimidazole resistance in Haemonchus contortus of goats from different geographic regions of Uttar Pradesh, India

Chandra, Subhash; Prasad, A.; Yadav, N.; Latchumikanthan, A.; Rakesh, R. L.; Praveen, Kaiser; Khobra, V.; Subramani, K.; Misri, Jyoti; Sankar, M.

doi:10.1016/j.vetpar.2015.01.005

Cited by 26 publications

(17 citation statements)

References 19 publications

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“…T. colubriformis and T. circumcincta , thereby, increasing the selection pressure on the resistant H. contortus genotypes leading to their predominance. Similar studies on BZ resistance have also been conducted in Rajasthan ( Tiwari et al, 2006 ); Uttarakhand ( Garg and Yadav, 2009 ); Maharashtra ( Ghalsasi et al, 2012 ) and UP, India ( Chandra et al, 2015 ). The results of molecular detection of BZ resistance are in correlation with FECRT employed for determining efficacy of FBZ in the present study.…”

Section: Resultsmentioning

confidence: 63%

“…There are many reports regarding BZ resistance in goats against GINs from others parts of the country like Uppal et al (1992) ; Ram et al (2007) ; Godara et al (2011) ; Manikkavasagan et al (2015) . The anthelmintic resistance survey of Chandra et al (2015) indicated that the status of BZ resistance is in alarming conditions in all parts of the Uttar Pradesh, India. In addition to MRCSG, Shahardar et al (2014) reported moderate resistance to FBZ at Govt.…”

Section: Resultsmentioning

confidence: 99%

“…AS-PCR, qPCR and pyrosequencing have been found to be more accurate in early diagnosis of BZ resistance as compared to FECRT or EHA ( Silvestre and Humbert, 2000 ; Coles et al, 2006 ; Barrere et al, 2013a , Barrere et al, 2013b ; dos Santos et al, 2014 ; Chandra et al, 2015 ). Molecular techniques could save time and money before going for application of BZ group of anthelmintics in a farm against which resistance has developed throughout the world ( Barrere et al, 2013b ).…”

Section: Resultsmentioning

confidence: 99%

“…The first report of anthelmintic resistance in India was by Varashney and Singh (1976) against phenothiazine and thiabandazole in H. contortus of sheep from Rishikesh (Uttarakhand). Subsequently anthelmintic resistance was reported in goat GINs from different parts of the country against different anthelmintics ( Uppal et al, 1992 ; Gill, 1996 ; Ram et al, 2007 ; Godara et al, 2011 ; Jaiswal et al, 2013 ; Chandra et al, 2015 ), however, there is no report from north most temperate region, Kashmir, of India, barring three reports in sheep GINs ( Nasreen et al, 2007 ; Itoo et al, 2012 ; Shahardar et al, 2014 ). In Jammu and Kashmir total small ruminant population is 6,195,000 ( Anonymous, 2007 ) with 267,000 goats in the Kashmir province ( Anonymous, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Multiple anthelmintic resistance in gastrointestinal nematodes of Caprines on Mountain Research Centre for Sheep and Goat at Kashmir Valley, India

Bihaqi

Allaie

Banday

et al. 2020

Parasite Epidemiology and Control

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The study was conducted to evaluate the status of anthelmintic resistance in Gastro-Intestinal Nematodes (GINs) of goats at an organized farm located in Kashmir, as there is no report of resistance against these parasites of goats from this temperate region, although it has been reported worldwide including India. Caprines reared at this farm exhibited reduced efficacy to multiple anthelmintics following treatments with Fenbendazole (FBZ), Closantel and Ivermectin (IVM) in Faecal Egg Count Reduction Test (FECRT). The results suggested that the overall efficacy was highest for IVM at 83.5% and 90.0% on 7 th and 14 th day post-treatment, respectively and least for FBZ at 44.3% and 62.5%, respectively, whereas the corresponding figures for closantel were 68.3% and 86.2%, respectively. The pre-treatment faecal culture revealed Haemonchus contortus , Teladorsagia circumcincta and Trichostrongylus colubriformis as predominant strongyles, however, in post-treatment samples, only H. contortus was observed. Further, the infective larvae were subjected to Allele specific PCR (AS-PCR) for accurate diagnosis of BZ resistance. The AS-PCR revealed 52% of H. contortus were homozygous resistant (rr) and 17% were heterozygous (rS) on day “0” before treatment and 100% homozygous resistant (rr) on 7th day post treatment. In both T. colubriformis and T. circumcincta, 100% population was homozygous susceptible (SS) at day “0” before treatment. The overall frequency of resistant (r) allele for H. contortus was 60.5% and for susceptible allele (s) was 39.5%. For T. colubriformis and T. circumcincta the frequency of susceptible allele (s) was 100%. The survey indicated that the GINs of goats on the farm have developed multiple anthelmintic resistance to FBZ, closantel and IVM and the condition is alarming in the farm. Moreover surveillance studies about status of anthelmintic resistance in other farms (Govternment as well as Private) of Kashmir valley should be carried out at large scale to develop effective and sustainable control strategies against GI Nematodes.

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Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple anthelmintic resistance in gastrointestinal nematodes of Caprines on Mountain Research Centre for Sheep and Goat at Kashmir Valley, India

Bihaqi

Allaie

Banday

et al. 2020

Parasite Epidemiology and Control

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“…With regard to anthelmintic resistance of GIN in goats, since the very first cases were reported in areas in the world such as New Zealand (Kettle et al, 1983), Australia (Barton et al, 1985), France (Kerboeuf and Hubert 1985), this problem is now as prevalent worldwide as in sheep (Kaplan 2004;Wolstenholme et al, 2004;Fleming et al, 2006;Jackson et al, 2012;Kaplan and Vidyashankar 2012;Nabukenya et al, 2014;Chandra et al, 2015). The prevalence is particularly high in Australia and South America, but reports of elevated prevalence in Europe are increasing (Váradi et al, 2011).…”

Section: Status Of Anthelmintic Resistance and Management Strategiesmentioning

confidence: 99%

Effects of dietary protein supplementation and plane of nutrition on the resistance and the resilience of Boer goats against artificial Haemonchus contortus infection under confined conditions

Can¹

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The effects of protein and/ or energy supplementation have been well studied in sheep. However, the few pen studies conducted with goats have failed to identify the role of protein supplementation and combinations with energy supplements against H. contortus infection.The general objective of this thesis was to investigate the effects of protein supplementation and plane of nutrition on resistance and resilience of Boer goats to infection from Haemonchus contortus. To achieve this the first experiment was designed to determine the appropriate amount of additional dietary protein (by having three groups of mature goats effectively fed three different levels of dietary protein) required by nematode free animals given a single relatively high dose of infective H. contortus larvae, to not become infected or at least develop resistance and/or resilience. It was assumed, based on the scientific literature, that a single relatively high dose of infective larvae would elicit strong pathogenic effects where additional dietary protein would give some assistance to the does with their defense to Haemonchus. As often is the case with research on goats the results were not unequivocally clear.Based on these results it was clear that a single dose of infective larvae was insufficient to become established and that these animals had already developed some level of immune response and thus it was assumed, again based on the scientific literature, to elicit strong pathogenic effects the animals tested needed to be younger (more naïve to Haemonchus) and with greater i.e. continued exposure to infective larvae as would happen under grazing conditions (trickle infection) such that different quantities of dietary protein would give these animals different responses to infection by Haemonchus. Again the responses by the goats only indicated subtle differences in immune responses against trickle H. contortus L3 infection. These results, again unexpected, indicated that level of intake (or more specifically plane of nutrition, prior to or during exposure) may be at least as important as protein supplementation in reasonably well fed animals; and or that the method of infection (oral drenching with L3 larvae) was possibly an unreliable method of infection requiring a more consistent method of infection. As such the third experiment involved nematode free mature animals (the only animals available at that time) fed on very different planes of nutrition preceding and during the experiment given relatively high trickle doses of H. contortus larvae via intra-ruminal infection.Overlaid across these experiments were constraints imposed on the amount of infective L3 larvae used to achieve infection; 100 L3 larvae per kg live-weight as a single dose was the maximum initially approved by the University Animal Ethics committee, as they had concerns about animals dying, they also stipulated the use of oral drenching for infective larvae. It was only after these methods failed ii to elicit a strong response was approval given by the Univers...

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Drug Resistance Assays for Parasitic Diseases

Légaré¹,

Ouellette²

2017

Antimicrobial Drug Resistance

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Status of benzimidazole resistance in Haemonchus contortus of goats from different geographic regions of Uttar Pradesh, India

Cited by 26 publications

References 19 publications

Multiple anthelmintic resistance in gastrointestinal nematodes of Caprines on Mountain Research Centre for Sheep and Goat at Kashmir Valley, India

Multiple anthelmintic resistance in gastrointestinal nematodes of Caprines on Mountain Research Centre for Sheep and Goat at Kashmir Valley, India

Effects of dietary protein supplementation and plane of nutrition on the resistance and the resilience of Boer goats against artificial Haemonchus contortus infection under confined conditions

Drug Resistance Assays for Parasitic Diseases

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