Status of insect resistance to<i>Bacillus thuringiensis</i>, mechanism and management -A review

Patel, S. I.; Patel, G. M.; Patel, Ramanuj; Patel, Arvind

doi:10.5958/j.0976-0741.34.3.008

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Another promising tool that could be used to confer CBB resistance is a proteinaceous inhibitor that was isolated from Lupinus bogotensis seeds, which showed effective biological activity against aspartic proteases (Molina et al 2010 ), digestive proteases that are also present in the CBB intestinal tract. Transgene pyramiding has been reported to positively affect insect control in cotton, rice, cabbage and other crops (Patel et al 2013 ; Yi et al 2013 ; Xu 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

Albuquerque

Bezerra

Romero

et al. 2015

Tropical Plant Biol.

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Genetic transformation of coffee (Coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. The transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (PHA-L) from Phaseolus vulgaris were characterized in genetically modified C. arabica expressing the α-amylase inhibitor-1 (α-AI1) gene. The α-AI1 inhibitor shows considerable activity toward digestive enzymes of the coffee berry borer (CBB) Hypothenemus hampei. This insect pest expends its life cycle almost entirely in coffee berries. Transgene containment in the fruit is important to meeting food and environmental safety requirements for releasing genetically modified (GM) crops. PCR analysis of T2 coffee plants showed a Mendelian single-copy segregation pattern. Ectopic transgene expression was only detected in coffee grains, as demonstrated by reverse transcription-PCR analysis of different plant tissues. An intense immunocytochemical signal associated with α-AI1 protein expression was localized to endospermic cells. In addition, a delay in the larval development of CBB was observed after challenging transgenic coffee seeds with the insect. These results indicate that the PHA-L promoter might be a useful tool in coffee for the seed-specific expression of genes related to coffee bean productivity, quality and pest protection. The biotechnological applicability of the α-AI1 gene for controlling CBB is also discussed. This work is the first report showing a seed-specific transgene expression in coffee plants.Electronic supplementary materialThe online version of this article (doi:10.1007/s12042-015-9153-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

Albuquerque

Bezerra

Romero

et al. 2015

Tropical Plant Biol.

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“…Distribution of CV was also having a high peak and long tail. Patel et al (2010) [20] discuss the method of fitting lognormal distribution to CV data of mustard crop experiments. Given a need to allow for, skewness and all unimodal distribution it has become interesting to study frameworks that are flexible enough to accommodate distributions with a broad range of properties.…”

Section: Introductionmentioning

confidence: 99%

Fitting of the distribution for CV value of the cotton and tobacco experiment

Nv¹,

Verma²,

Nb³

et al. 2020

J Pharmacogn Phytochem

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Coefficient of variation (CV) was a measure commonly applied to present variation in agricultural experiments. Its merits are well known, most important being one that CV deals with what we could call the scale-invariant variability in the experiments. It was easier to understand than variance it was based upon. Coefficient of variation used to compare the variation of traits in two (or more) populations or, more commonly, the variation of different traits in a population of the study. If the CV was within certain limits one can say that block has homogeneity in the character under study. Conditional on which distribution was to be used for modelling of the CV data of an experiment, which was a common problem in agricultural science. The six distributions viz., Normal, Lognormal, Gamma, Weibull, Exponential and Beta used for the fitting of distribution study. The test statistic Kolmogorov-Smirnov test, Cramer-Von-Mises test, Anderson-Darling test and Chi-Square test for each data set was computed for six probability distributions and used to identify the best-fit distribution. The probability distributions viz., Normal, Lognormal, Gamma, Beta, Weibull, Exponential were identifying to evaluate the best-fit probability distribution for CV value. In addition, the different forms of these distributions also tried and thus the probability distributions applied to find out the best-fit probability distribution and to know the nature and shape of the distribution.

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Using Agent-Based Modelling to Predict the Role of Wild Refugia in the Evolution of Resistance of Sea Lice to Chemotherapeutants

et al. 2015

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A major challenge for Atlantic salmon farming in the northern hemisphere is infestation by the sea louse parasite Lepeophtheirus salmonis. The most frequent method of controlling these sea louse infestations is through the use of chemical treatments. However, most major salmon farming areas have observed resistance to common chemotherapeutants. In terrestrial environments, many strategies employed to manage the evolution of resistance involve the use of refugia, where a portion of the population is left untreated to maintain susceptibility. While refugia have not been deliberately used in Atlantic salmon farming, wild salmon populations that migrate close to salmon farms may act as natural refugia. In this paper we describe an agent-based model that explores the influence of different sizes of wild salmon populations on resistance evolution in sea lice on a salmon farm. Using the model, we demonstrate that wild salmon populations can act as refugia that limit the evolution of resistance in the sea louse populations. Additionally, we demonstrate that an increase in the size of the population of wild salmon results in an increased effect in slowing the evolution of resistance. We explore the effect of a population fitness cost associated with resistance, finding that in some cases it substantially reduces the speed of evolution to chemical treatments.

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Status of insect resistance toBacillus thuringiensis, mechanism and management -A review

Cited by 3 publications

References 29 publications

Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

Fitting of the distribution for CV value of the cotton and tobacco experiment

Using Agent-Based Modelling to Predict the Role of Wild Refugia in the Evolution of Resistance of Sea Lice to Chemotherapeutants

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