Steam Distillation of Volatile Fatty Acids in Rumen Ingesta

Kromann, R. P.; Meyer, James H.; Stielau, W. J.

doi:10.3168/jds.s0022-0302(67)87356-9

Cited by 57 publications

(18 citation statements)

References 9 publications

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“…This value was subtracted from the total N and was designated as TCA ppt-N (Garg and Gupta 1992). Total VFA content in rumen liquor was determined by using the Markham's steam distillation apparatus following the principles of Kromann et al (1967). The rumen ingesta of 100 g was collected and incinerated in the distillation apparatus.…”

Section: In Situ Studymentioning

confidence: 99%

Effect of supplementation of mustard oil cake on intake, digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

Khandaker

Uddin

Sultana³

et al. 2011

Trop Anim Health Prod

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The objective of this study was to analyse the effects of different levels of rumen-degradable protein (RDP) on intake, digestibility and microbial protein synthesis by supplementing mustard oil cake (MOC) on rice straw-based diet of cattle (Bos indicus) in Bangladesh. A 4 × 4 Latin square design was applied. Four diets having constant energy (7.0 MJ/kg of dry matter (DM)) with varying levels of RDP (M(0) = 4.1 g/MJ (control), M(1) = 6.3 g/MJ, M(2) = 8.3 g/MJ and M(3) = 12.4 g/MJ of metabolizable energy (ME)) were received by each animal for a period of 28 days. A metabolism trial was conducted for 7 days. Results indicate that with increasing levels of RDP, crude protein (CP) and RDP intake increased significantly (P < 0.01). The significant (P < 0.01) increase in digestibility values are obtained for DM, organic matter, CP and digestible organic matter in the rumen. The digestibility of neutral detergent fibre and acid detergent fibre was also increased significantly (P < 0.05). The total nitrogen (N), ammonia-N and total volatile fatty acids increase significantly (P < 0.01) while the rumen pH increased from M(0) to M(2) and decreased thereafter. The efficiency microbial N intake increased significantly (P < 0.01) but showed a curvilinear response with higher RDP level (12.40 g/RDP/MJ ME). This study concludes that supplementation of RDP from MOC enhances the intake, digestibility and microbial protein synthesis which ultimately increases utilization of low-quality feed resources that can be used for developing cost-effective feeding systems on a straw-based diet in tropical regions.

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Section: In Situ Studymentioning

confidence: 99%

Effect of supplementation of mustard oil cake on intake, digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

Khandaker

Uddin

Sultana³

et al. 2011

Trop Anim Health Prod

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“…Ruminal pH was determined using a digital pH meter, NH 3 -N was determined according to Abou-Akkada and El-Shazly (1964). Ruminal total volatile fatty acids (TVFA ' s) concentrations were determined by steam distillation according to Kromann et al (1967).…”

Section: Sampling Of Rumen Liquormentioning

confidence: 99%

Bio-Detoxification of Jojoba Meal by Aspergillus oryzae and Impact of its Utilization in Ewes and Lambs' Feeding

Abdou

2018

Journal of Animal and Poultry Production

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The present study was conducted at Maryout Research Station, Desert Research Center, Egypt. Jojoba meal (JM) was treated biologically with Aspergllus orgyzae Fk-923 to study its effects on performance, digestibility coefficient, nutritive value, blood metabolites, and rumen characteristic of sheep. Total of 30 Barki ewes aged 3-4 years old with an average weight of 33.46 kg were randomly allotted into three experimental treatments (10 ewes of each) to study the reproductive traits of Barki sheep. The experiment lasted for 150 days to cover the pregnancy period. After weaning eighteen growing lambs with average live body weight of 15.41kg, were used in feeding trial 210 days (six lambs of each). The experimental rations (R1, R2 and R3) of treated jojoba meal (TJM) at level of (0, 7% and 14%) as a replacement of cotton seed meal, respectively. The results indicated that the analysis of experimental rations' components demonstrated that the experimental rations were similar in its chemical composition but there was a slight increase in the crude protein content with increasing the percentage of jojoba meal (for the slight decrease in CF content). During early pregnancy, ewes consumed 14% of treat jojoba meal had lower total crude fiber intake than those of R1 (control) and R2 groups. During late pregnancy, the intake levels of total dry matter, crude protein and crude fiber were higher in lambs feed the control ration compared to both 7 and 14% (TJM). During the early pregnancy and late pregnancy, changes in body weight of sheep were not significantly affected by type of diets. Digestibility coefficients by lambs fed 14% JM (R3) showed numerically higher digestion values of DM, OM, CP, CF, NFE and NDF followed by those feed 7% JM (R2) than the R1 (control). Lambs fed jojoba meal had higher TDN and the digestible crude protein (DCP) improved with increasing percentage of JM. Also nitrogen balance as % of nitrogen intake was higher in animals fed R3 and the lowest value was in R2. Feed conversion values as DM, TDN and DCP were improved for lambs fed the control followed by rations containing 7 and 14% JM treated with fungi, respectively. Providing treated JM with fungi (R2 and R3) improved the pH values. The opposite trend was observed for rumen ammonia concentrations (NH 3-N) and total volatile fatty acids values (TVFA's), which found to be low before feeding, then increased at 3 hrs. post feeding and returned to decrease at 6 hrs. post feeding but NH 3-N levels increased at 6 hrs. post feeding for R1 and R3. There were no significant (P>0.05) differences in creatinine, total protein, albumin, globulin, AST, ALT, T3 and T4 levels. The economic parameters for R3 was the best followed by R2 compared with control ration (R1). It could be concluded that 14% treated JM with fungus can be used in growing lambs' ration.

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“…Nilai energi ditetapkan dengan bomb kalorimeter dan protein dengan cara Kjeldhal. Konsentrasi VFA total dengan cara destilasi uap (Kroomann et al, 1967)dan parsialnya dengan kromatografi gas, sedangkan konsentrasi N-NH3, diukur dengan cawan Conway (1947).…”

Section: Peubah Respon Koleksi Data Dan Analisisunclassified

Biotransformasi Limbah Ikan Menjadi Bahan Pakan untuk Ruminansia

2012

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Fish waste (head, viscera and tails) wich ensilage with saccharomyces sp and lactobacillus sp, had a high quality and protein content of about 53,18 ± 0,83% and a good aroma. An experiment have been conducted by experimental method with in sacco trial and in vivo trial. The treatment tested were 4 kind of protein source : (1) fish waste silages mixed with tapioca ; (2) fish waste silages mixed with rice bran ; (3) soy bean kernels and (4) fish meal. The in sacco trial was carried out on 3 fistulated cows, to study the rate of protein degradability in the rumen. The in vivo trial was carried out by Completely Randomized Design on 16 heads of fat tail rams. The variable measured were: rumen fermentation product (VFA and N-NH 3 ); energy and protein digestibility; daily body weight gain of the rams. The experiment showed, that the source of protein use have no significantly effect upon VFA both on the VFA total and individual VFA (acetate, propionate and butyrate). The highest propionate reach by soya bean kernels (P < 0.01). Based on the all variables measured, it was indicated the silage of fish waste can be used to substituted of soya bean kernels or fish meal in the rationof sheep.

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Steam Distillation of Volatile Fatty Acids in Rumen Ingesta

Cited by 57 publications

References 9 publications

Effect of supplementation of mustard oil cake on intake, digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

Effect of supplementation of mustard oil cake on intake, digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

Bio-Detoxification of Jojoba Meal by Aspergillus oryzae and Impact of its Utilization in Ewes and Lambs' Feeding

Biotransformasi Limbah Ikan Menjadi Bahan Pakan untuk Ruminansia

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