Steamed Panax notoginseng Attenuates Anemia in Mice With Blood Deficiency Syndrome via Regulating Hematopoietic Factors and JAK-STAT Pathway

Zhang, Zejun; Zhang, Yiming; Gao, Min; Cui, Xiuming; Yang, Yang; Duijn, Bert van; Wang, Mei; Hu, Yupiao; Wang, Chengxiao; Xiong, Yin

doi:10.3389/fphar.2019.01578

Cited by 33 publications

(23 citation statements)

References 74 publications

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“… 2009 ; Zhang et al. 2019 ). Our results indicated that SXF increased the splenic index, and histopathological tests also showed that SXF increased the number of splenic red lineage cells and promoted the recovery of splenic function after CTX injury.…”

Section: Discussionmentioning

confidence: 99%

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Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

Dou

Gong

et al. 2021

Pharmaceutical Biology

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Context Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. Objective To study the effect and possible mechanism of SXF to restore haematopoietic function. Materials and methods Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10–12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis. Results Red blood cell relative changes in the SXF group (low: −5.50 ± 1.58%; medium: −11.11 ± 4.15%; high: −8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all p < 0.01) in female mice. Haemoglobin and red blood cell-specific volume showed the same trend. However, SXF did not have significant effects on male mice. Splenic index in the medium group (4.44 ± 0.46%) relative negative control (3.38 ± 0.10%) significantly improved ( p < 0.01) in female mice. Using network pharmacology, 77 active components and 337 targets were screened from SXF. These targets are closely related to the mitogen-activated protein kinase pathway. Conclusions SXF has good clinical application potential. However, the mechanism requires in-depth research. Our findings are of great significance in anaemia treatment and provide a new perspective for Chinese medicine research.

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Section: Discussionmentioning

confidence: 99%

“…In recent years, with the popularization of systems biology, network pharmacology has become an important tool for analyzing the performance of TCM (Zhang et al. 2019 ; Cui et al. 2020 ; Sinan et al.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

Dou

Gong

et al. 2021

Pharmaceutical Biology

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“…It is a virtual Chinese medicine (TCM) with an annual output value exceeded 70 billion RMB yuan and more than 3600 related drug approval numbers in China 1 . Its main efficacy is promoting blood circulation, removing blood stasis, detumescence and analgesia 2 – 4 .…”

Section: Introductionmentioning

confidence: 99%

Identification of geographical origins of Panax notoginseng based on HPLC multi-wavelength fusion profiling combined with average linear quantitative fingerprint method

Bai

Pan

Dong

et al. 2021

Sci Rep

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The aim of this study was to establish a method for geographical origins identification of Panax notoginseng (P. notoginseng) based on abundant chromatographic spectral information. Characteristic fingerprints of P. notoginseng extracts samples were generated by Multi-wavelength Fusion Profiling (MWFP) method based on the HPLC fingerprints established at three wavelengths of 203 nm, 270 nm and 325 nm. The samples grouping results calculated with the averagely linear quantified fingerprint method (ALQFM) and the unsupervised statistical methods based on fusion fingerprints matches with the geographical origins. The Multi-wavelength Fusion Profiling (MWFP) method has been successfully applied to identification of geographical origins of P. notoginseng and shows the advantages compared with single—channel fingerprints. In addition, eight physiologically active components, including four saponins, two flavones and two amino acids, were identified from the most relevant ingredients of P. notoginseng geographical origins by fusion fingerprint-efficacy relationship analysis. Besides the recognized active saponins, other categories of active ingredients such as flavonoids and amino acids should be paid attention to in the producing areas identification or the quality judgment of P. notoginseng.

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“…Steaming is one of the processing methods of traditional Chinese medicine, in which the purified Chinese medicine along with water is put into a steamed container and the mixture heated to a certain degree. Steamed notoginseng has increased potential for medicinal use, which is due to some unique less‐polar saponins that are generated during steaming (Zhang et al, 2019). Inspired by the steaming process of notoginseng, we herein tried to establish a steaming protocol to treat SLS in order to maximize its anti‐acetylcholinesterase activity, which is helpful to develop the source of stem‐leaf of P. notoginseng for medicinal use.…”

Section: Introductionmentioning

confidence: 99%

Dynamic changes in chemical compositions and anti‐acetylcholinesterase activity associated with steaming process of stem‐leaf saponins of Panax notoginseng

Guan

Lin

et al. 2021

Biomedical Chromatography

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Stem‐leaf saponins (SLSs), the total saponins from aerial part of P. notoginseng, are by‐products of notoginseng, a famous traditional Chinese medicine. SLSs have been used as a health functional food in China, but its mild effects limited clinical applications in diseases. Inspired by steaming of notoginseng to enhance its pharmacological activity, a steaming protocol was developed to treat SLSs. SLSs were steamed at 100, 120, and 140°C for 1, 2, 3, and 4 h, respectively. The ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight MS and ultra‐performance liquid chromatography–tandem triple quadrupole mass spectrometry were applied to analyze the dynamic changes in chemical compositions. The anti‐acetylcholinesterase activity of steamed SLS were assessed in vitro by directly determining the metabolic product of acetylcholine/choline. The components of SLSs were significantly changed by steaming. A total of 117 saponins and aglycones were characterized, and 35 of them were newly generated. The anti‐acetylcholinesterase activity of steamed SLSs gradually increased with the extension of steamed time and the increase of steamed temperature and reached the maximum after 140°C for 3 h. Furthermore, ginsenosides Rk1 and Rg5, the main components of steamed SLSs, showed dose‐dependent anti‐acetylcholinesterase activities with half maximal inhibitory concentration (IC50) values of 26.88 ± 0.53 μm and 22.41 ± 1.31 μm that were only 1.8‐ and 1.5‐fold higher than that of donepezil with IC50 values of 14.93 ± 4.17 μM, respectively.

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Steamed Panax notoginseng Attenuates Anemia in Mice With Blood Deficiency Syndrome via Regulating Hematopoietic Factors and JAK-STAT Pathway

Cited by 33 publications

References 74 publications

Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

Identification of geographical origins of Panax notoginseng based on HPLC multi-wavelength fusion profiling combined with average linear quantitative fingerprint method

Dynamic changes in chemical compositions and anti‐acetylcholinesterase activity associated with steaming process of stem‐leaf saponins of Panax notoginseng

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