Stearate Preferentially Induces Apoptosis in Human Breast Cancer Cells

Evans, Lynda M.; Cowey, Stephanie; Siegal, Gene P.; Hardy, Robert W.

doi:10.1080/01635580902825597

Cited by 79 publications

(65 citation statements)

References 31 publications

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“…The only significant finding was the difference in IC 50 between the PE-SLNs-optimized formula and the void-optimized formula on the MCF-7 cell line. These results could be attributed to the effect of stearic acid, the main component of our SLNs, which has been previously reported for its anticancer effect by inducing apoptosis on breast cancer, 51 prostate cancer particularly on the PC-3 cell line 52 and liver cancer.…”

mentioning

confidence: 82%

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

Badawi¹,

Teaima²,

El-Say³

et al. 2018

IJN

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Background Pomegranate extract (PE) is a natural product with potent antioxidant and anticancer activity because of its polyphenols content. The main purpose of this study was to maximize the PE chemotherapeutic efficacy by loading it in an optimized solid lipid nanoparticles (SLNs) formula. Materials and methods The influence of independent variables, which were lipid concentration (X 1 ), surfactant concentration (X 2 ) and cosurfactant concentration (X 3 ), on dependent ones, which were particle size (Y 1 ), polydispersity index (Y 2 ), zeta potential (Y 3 ), entrapment efficiency (Y 4 ) and cumulative % drug release (Y 5 ), were studied and optimized using the Box–Behnken design. Fifteen formulations of PE-SLNs were prepared using hot homogenization followed by ultra-sonication technique. Response surface plots, Pareto charts and mathematical equations were produced to study the impact of independent variables on the dependent quality parameters. The anti-proliferative activity of the optimized formula was then evaluated in three different cancer cell lines, namely, MCF-7, PC-3 and HepG-2, in addition to one normal cell line, HFB-4. Results The results demonstrated that the particle sizes ranged from 407.5 to 651.9 nm and the entrapment efficiencies ranged from 56.02 to 65.23%. Interestingly, the 50% inhibitory concentration of the optimized formula had more than a 40-fold improved effect on the cell growth inhibition in comparison with its free counterpart. Furthermore, it was more selective against cancer cells than normal cells particularly in MCF-7 breast cancer cells. Conclusion These data proved that nanoencapsulation of PE enhanced its anticancer efficacy. Therefore, our results suggested that a PE-loaded SLNs optimized-formula could be a promising chemo therapeutic agent.

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mentioning

confidence: 82%

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

Badawi¹,

Teaima²,

El-Say³

et al. 2018

IJN

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“…However, it has been determined that inhibition of DAG synthesis with Triacsin C completely reversed stearate-induced caspase 3 activity in two breast cancer cell lines [82]. Caspase 3 is known for its apoptotic functions, showing that DAG somehow is involved in cell survival during breast cancer.…”

Section: Consensus In Literature But Few Studies On Sosmentioning

confidence: 99%

Role of ESR Pathway Genes in Breast Cancer: A Review

Kumar¹,

Myers²,

Homsi³

et al. 2018

ABCR

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Breast cancer is the leading cause of death in women. Prognosis of breast cancer is often pessimistic because the tumors are prone to metastasizing to the bone, brain, and lung. The estrogen signaling receptor (ESR) pathway contains 39 main genes and proteins which makes it one of the larger signaling pathways. Predominately this pathway and the proteins within are involved in breast growth and development, making it a prospective area of study for breast cancer. While the healthy ESR pathway has been constructed and is well established, a mechanistic model of mutated genes of ESR pathway has not been delved upon. Such mutated models could be utilized for selecting combinational targets for drug therapies, as well as elucidating crosstalk between other pathways and feedback mechanisms. To construct the mutated models of the ESR pathway it is imperative to assess what is currently understood in the literature and what inconsistencies exist in order to resolve them. Without this information, a model of the ESR pathway will be unreliable and likely unproductive. This review is the detailed literature survey of the biological studies performed on ESR pathways genes, and their respective roles in breast cancer. Furthermore, the details mentioned in the review can be beneficial for the integrated study of the ESR pathway genes, which includes, structural and dynamics study of the genes products, to have a holistic understanding of the cancer mechanism.

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“…Furthermore, 10-25 μM stearate is known to induce translocation of Annexin II to detergent resistant membrane in the Hs578t cells, hypothetically affecting cellular signaling through modifications of lipid rafts and protein acylation, although the effect on the cancer cell phenotype is not known [59]. As with palmitate, 25-150 μM stearate has been shown to preferentially inhibit growth and induce apoptosis of breast cancer cells, potentially through the induction of de novo diacyglycerol synthesis and activation of protein kinase C [46,57,60,61]. Interestingly, the apoptotic effect of stearate appears to be cancer cell specific as treatment of non-transformed breast cells has no effect on cell survival [60,61].…”

Section: Stearatementioning

confidence: 99%

“…As with palmitate, 25-150 μM stearate has been shown to preferentially inhibit growth and induce apoptosis of breast cancer cells, potentially through the induction of de novo diacyglycerol synthesis and activation of protein kinase C [46,57,60,61]. Interestingly, the apoptotic effect of stearate appears to be cancer cell specific as treatment of non-transformed breast cells has no effect on cell survival [60,61].…”

Section: Stearatementioning

confidence: 99%

Optimizing Dietary Fat to Reduce Breast Cancer Risk: Are we there Yet?

Evans¹,

Hardy²

2014

TOBCANJ

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For over 70 years, long chain fatty acids have been implicated in the development and progression of breast cancer. Although the exact role remains to be elucidated, dietary factors have been implicated in approximately 35% of cancer deaths. Currently, biomarker, animal and in vitro studies suggest that the individual fatty acids have differing roles in the promotion or prevention of breast cancer development and progression. The goal of this review is to assess epidemiological, animal and cellular studies with respect to the role of dietary long chain fatty acids in breast cancer risk. Subsequently we identify the common findings in these studies, discuss important factors that may influence human studies and evaluate the current dietary fat recommendations with respect to these findings.

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Stearate Preferentially Induces Apoptosis in Human Breast Cancer Cells

Cited by 79 publications

References 31 publications

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study

Role of ESR Pathway Genes in Breast Cancer: A Review

Optimizing Dietary Fat to Reduce Breast Cancer Risk: Are we there Yet?

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