Stem and Progenitor Cells of Dental and Gingival Tissue Origin

Morsczeck, Christian; Huang, George T.‐J.; Shi, Songtao

doi:10.1002/9781118498026.ch15

Cited by 13 publications

(8 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were grown in media containing α-modification of Eagle's medium (α-MEM) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin-G, 100 mg/mL streptomycin, and 0.25 mg/mL fungizone (Gemini Bio-Products, Inc., West Sacramento, CA, USA) and incubated at 37°C in 5% CO2. These OSCs are heterogeneous population of cells expressing typical MSC markers 13,15 . The above mentioned isolation method routinely gives rise to clonogenic MSCs capable of expansion with robust proliferation in cultures [23][24][25][26] .…”

Section: Cell Culturementioning

confidence: 99%

“…OSCs originate from migrating cranial neural crest cells 11 , therefore they are thought to have a greater neurogenic potential than other MSCs 6,12 . Human OSCs include those isolated from discarded wisdom teeth such as dental pulp stem cells (DPSCs) and stem cells from apical papilla (SCAP) 13 , or from excised gingival tissue which harbors gingivaderived mesenchymal stem cells (GMSCs) [14][15][16] . These OSCs are different from other MSCs in many aspects.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Liu

Zou

El-Ayachi

et al. 2018

Stem Cell Rev and Rep

Self Cite

View full text Add to dashboard Cite

Center (UTHSC) based on exempt protocols approved by the respective Medical Institutional Review Board (BU: #H-28882; and UTHSC:#12-01937-XM). • Consent for publication: This manuscript has been approved by all authors and is solely the work of the authors named. • Availability of data and material: All data and information of the materials relevant to this project are available upon request. Cell lines established and used in the project will be available upon request.

show abstract

Section: Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Liu

Zou

El-Ayachi

et al. 2018

Stem Cell Rev and Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…To generate iPSCs, dental stem cells (DSCs) or cells from oral mucosa have been considered one of the most accessible and feasible source to produce iPSCs (Huang, 2010, Tamaoki , et al , 2010, Wada , et al , 2011, Yan , et al , 2010, Zou , et al , 2012). DSCs normally are obtained from discarded wisdom teeth such as dental pulp stem cells (DPSCs) and stem cells from apical papilla (SCAP) (Huang , et al , 2009, Morsczeck , et al , 2013). ) Previously we have successfully generated iPSCs from various human DSCs (Yan , et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

Human dental stem cell derived transgene‐free iPSCs generate functional neurons via embryoid body‐mediated and direct induction methods

Ayachi

Zhang

Zou

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Induced pluripotent stem cells (iPSCs) give rise to neural stem/progenitor cells, serving as a good source for neural regeneration. Here, we established transgene-free (TF) iPSCs from dental stem cells (DSCs) and determined their capacity to differentiate into functional neurons in vitro. Generated TF iPSCs from stem cells of apical papilla and dental pulp stem cells underwent two methods-embryoid body-mediated and direct induction, to guide TF-DSC iPSCs along with H9 or H9 Syn-GFP (human embryonic stem cells) into functional neurons in vitro. Using the embryoid body-mediated method, early stage neural markers PAX6, SOX1, and nestin were detected by immunocytofluorescence or reverse transcription-real time polymerase chain reaction (RT-qPCR). At late stage of neural induction measured at Weeks 7 and 9, the expression levels of neuron-specific markers Nav1.6, Kv1.4, Kv4.2, synapsin, SNAP25, PSD95, GAD67, GAP43, and NSE varied between stem cells of apical papilla iPSCs and H9. For direct induction method, iPSCs were directly induced into neural stem/progenitor cells and guided to become neuron-like cells. The direct method, while simpler, showed cell detachment and death during the differentiation process. At early stage, PAX6, SOX1 and nestin were detected. At late stage of differentiation, all five genes tested, nestin, βIII-tubulin, neurofilament medium chain, GFAP, and Nav, were positive in many cells in cultures. Both differentiation methods led to neuron-like cells in cultures exhibiting sodium and potassium currents, action potential, or spontaneous excitatory postsynaptic potential. Thus, TF-DSC iPSCs are capable of undergoing guided neurogenic differentiation into functional neurons in vitro, thereby may serve as a cell source for neural regeneration.

show abstract

“…For more than 10 years, human dental stem cell research has focused on the identification and characterization of human stem/progenitor cell populations, which can be isolated, for example, from retained third molars of juvenile patients [ 3 ]. One example for such type of dental stem cells are undifferentiated cells from the dental follicle (DFCs) [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundDental stem cells in combination with implant materials may become an alternative to autologous bone transplants. For tissue engineering different types of soft and rigid implant materials are available, but little is known about the viability and the osteogenic differentiation of dental stem cells on these different types of materials. According to previous studies we proposed that rigid bone substitute materials are superior to soft materials for dental tissue engineering.MethodsWe evaluated the proliferation, the induction of apoptosis and the osteogenic differentiation of dental stem/progenitor cells on a synthetic bone-like material and on an allograft product. The soft materials silicone and polyacrylamide (PA) were used for comparison. Precursor cells from the dental follicle (DFCs) and progenitor cells from the dental apical papilla of retained third molar tooth (dNC-PCs) were applied as dental stem cells in our study.ResultsBoth dental cell types attached and grew on rigid bone substitute materials, but they did not grow on soft materials. Moreover, rigid bone substitute materials only sustained the osteogenic differentiation of dental stem cells, although the allograft product induced apoptosis in both dental cell types. Remarkably, PA, silicone and the synthetic bone substitute material did not induce the apoptosis in dental cells.ConclusionsOur work supports the hypothesis that bone substitute materials are suitable for dental stem cell tissue engineering. Furthermore, we also suggest that the induction of apoptosis by bone substitute materials may not impair the proliferation and the differentiation of dental stem cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s40729-014-0002-y) contains supplementary material, which is available to authorized users.

show abstract

Stem and Progenitor Cells of Dental and Gingival Tissue Origin

Cited by 13 publications

References 55 publications

Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Human dental stem cell derived transgene‐free iPSCs generate functional neurons via embryoid body‐mediated and direct induction methods

Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions

Contact Info

Product

Resources

About