Learning media development plays an important role in nursing training. This study aims to: 1) evaluate the feasibility of project-based flipped learning media in nursing training, and 2) analyze differences in critical thinking ability (CTA), creative thinking (CT), and spiritual attitudes (SA) of nurses between those who practice using project-based flipped learning (PjBFL) media and those who practice with conventional online media. This research uses a mixed-method approach. The first phase is developing learning media and conducting interviews with research subjects, then conducting experiments with a quasi-experimental pretest and posttest control group design. The learning media developed are electronic modules and learning videos. Learning media has been validated by 5 learning media experts and 5 learning content experts. The results of the expert validation were then carried out in one-to-one trials involving 3 nurses, small group trials involving 9 nurses, and one-class trials. The results of the implementation showed that the posttest scores for CTA, CT, and SA of nurses were higher than the pretest scores. The results of multivariate testing showed that there were differences in the results of CTA, CT, and SA between those who practiced PjBFL media and conventional online nursing training. As a conclusion, this research has produced learning media products based on PjBFL that are feasible, effective, and have advantages as learning media for use in nursing training. The superiority of PjBFL media is proven by the experimental results that PjBFL media has a higher effect on the CTA, CT, and SA of nurses.