Stem Cell-Based Microphysiological Osteochondral System to Model Tissue Response to Interleukin-1β

Lin, Hang; Lozito, Thomas P.; Alexander, Peter G.; Gottardi, Riccardo; Tuan, Rocky S.

doi:10.1021/mp500136b

Cited by 123 publications

(119 citation statements)

References 39 publications

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“…In the case of the enthesis, bone media, cartilage media, ligamentous/tendinous media, and meniscal media are typically used individually or in combination. The majority of osteogenic media contains β-glycerophosphate and dexamethasone, [51,54,102,153–155] with some including BMP-2 [153,155] and TGF-β. [102] Dexamethasone is also included in some chondrogenic media, [154–156] while TGF-β is often used in media for the soft tissue portions of the enthesis.…”

Section: Construct Maturationmentioning

confidence: 99%

“…[102] Dexamethasone is also included in some chondrogenic media, [154–156] while TGF-β is often used in media for the soft tissue portions of the enthesis. [51,102,153–156] Dexamethasone is a glucocorticoid that has been shown to increase ALP activity in MSCs. [157] β-glycerophosphate is an organic phosphate donor classically used to induce MSC differentiation towards bone phenotypes.…”

Section: Construct Maturationmentioning

confidence: 99%

“…[157] β-glycerophosphate is an organic phosphate donor classically used to induce MSC differentiation towards bone phenotypes. [51,153,158,159] Application of β-glycerophosphate at the interface of a calcium polyphosphate substrate cultured with chondrocytes formed two zones, a calcified region between a calcium phosphate bony substrate and a hyaline cartilage-like zone. [44] Furthermore, combining β-glycerophosphate and BMP-2 in the bone region of an osteochondral scaffold directed stem cell osteogenesis.…”

Section: Construct Maturationmentioning

confidence: 99%

“…Copyright © 2014 John Wiley & Sons Ltd. [163] (B) Osteochondral bioreactor. [153] Chondrogenic and osteogenic media are continuously flowed through a scaffold, while maintaining separation of media baths through use of an O-ring. Histology shows interfacial region for construct: chondral component (CC) and osseous component (OC).…”

Section: Figmentioning

confidence: 99%

“…Further permissions related to the material excerpted should be directed to the ACS. [153] (C) Combination mechanical, chemical, and co-culture bioreactor for culturing meniscal enthesis constructs. These constructs consist of two bone plugs seeded with mesenchymal stem cells (MSCs) injected and connected with a high density collagen gel embedded with meniscal fibrochondrocytes (FCCs).…”

Section: Figmentioning

confidence: 99%

See 4 more Smart Citations

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

et al. 2017

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Soft tissue-to-bone interfaces are complex structures that consist of gradients of extracellular matrix materials, cell phenotypes, and biochemical signals. These interfaces, called entheses for ligaments, tendons, and the meniscus, are crucial to joint function, transferring mechanical loads and stabilizing orthopedic joints. When injuries occur to connected soft tissue, the enthesis must be re-established to restore function, but due to structural complexity, repair has proven challenging. Tissue engineering offers a promising solution for regenerating these tissues. This prospective review discusses methodologies for tissue engineering the enthesis, outlined in three key design inputs: materials processing methods, cellular contributions, and biochemical factors.

show abstract

Section: Construct Maturationmentioning

confidence: 99%

Section: Construct Maturationmentioning

confidence: 99%

Section: Construct Maturationmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

See 3 more Smart Citations

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

et al. 2017

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Designer Scaffolds for Interfacial Bioengineering

et al. 2023

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In regenerative medicine, the healing of the interfacial zone between tissues is a major challenge, yet approaches for studying the complex microenvironment of this interface remain lacking. Herein, these complex living interfaces by manufacturing modular “blocks” of naturally porous decellularized plant‐derived scaffolds with a computer numerical controlled mill are studied. How each scaffold can be seeded with different cell types and easily assembled in a manner akin to LEGO bricks to create an engineered tissue interface (ETI) is demonstrated. Cells migrate across the interface formed between an empty scaffold and a scaffold preseeded with cells. However, when both scaffolds contain cells, only a shallow cross‐over zone of cell infiltration forms at the interface. As a proof‐of‐concept study, ETIs to investigate the interaction between lab grown bone and connective tissues are used. Consistent with the above, a cross‐over zone of the two distinct cell types forms at the interface between scaffolds, otherwise the populations remain distinct. Finally, how ETIs are biocompatible in vivo are demonstrated, becoming vascularized and integrated into surrounding tissue after implantation. Herein, new tissue design avenues for understanding biological processes or the development of synthetic artificial tissues are created.

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Biochemical Gradients to Generate 3D Heterotypic‐Like Tissues with Isotropic and Anisotropic Architectures

Canadas

Ren

Marques

et al. 2018

Adv Funct Materials

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Anisotropic 3D tissue interfaces with functional gradients found in nature are replicated in vitro for drug development and tissue engineering. Even though different fabrication techniques, based on material science engineering and microfluidics, are used to generate such microenvironments, mimicking the native tissue gradient is still a challenge. Here, the fabrication of 3D structures are described with linear/random porosity and gradient distribution of hydroxyapatite microparticles which are combined with a gradient of growth factors generated by a dual chamber for the development of heterotypiclike tissues. The hydroxyapatite gradient is formed by applying a thermal ramp from the first to the second gel layer, and the porous architecture is controlled through ice templating. A 3D osteochondral (OC) tissue model is developed by codifferentiating fat pad adipose-derived stem cells. Osteogenic and chondrogenic markers expression is spatially controlled, as it occurs in the native osteochondral unit. Additionally, a prevasculature is spatially induced by the perfusion of proangiogenic medium in the bone-like region, as observed in the native subchondral bone. Thus, in this study, precise spatial control is developed over cell/tissue phenotype and formation of prevasculature which opens up possibilities for the study of complex tissues interfaces, with broader applications in drug testing and regenerative medicine.

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Stem Cell-Based Microphysiological Osteochondral System to Model Tissue Response to Interleukin-1β

Cited by 123 publications

References 39 publications

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

Designer Scaffolds for Interfacial Bioengineering

Biochemical Gradients to Generate 3D Heterotypic‐Like Tissues with Isotropic and Anisotropic Architectures

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