Stem cell differentiation on conducting polyaniline

Humpolíček, Petr; Radaszkiewicz, Katarzyna Anna; Kašpárková, Věra; Stejskal, Jaroslav; Trchová, Miroslava; Kuceková, Zdenka; Vičarová, Hana; Pachernı́k, Jiřı́; Lehocký, Marián; Minařík, Antonín

doi:10.1039/c5ra12218j

Cited by 37 publications

(21 citation statements)

References 37 publications

(59 reference statements)

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“…Consequently, it can be concluded that introducing PAMPSA into the polymer bulk during synthesis notably affected NIH/3T3 cell proliferation in comparison with reference and pristine polyaniline. These results correspond to observing the preferable behavior of mouse embryonic stem cells on pristine PANI forms, in comparison with PANI deposited with PAMPSA [ 14 ].…”

Section: Resultssupporting

confidence: 83%

“…An interesting phenomena was observed for polyaniline samples that contained PAMPSA in the reaction mixture. As previously published, a significant decrease in γ tot occurred when pristine polyaniline substrate was doped with PAMPSA [ 14 ]. In the present study, in the case of PANI-PAMPSA-1:1, the values obtained for the surface energy corresponded to PANI doped with PAMPSA.…”

Section: Resultsmentioning

confidence: 53%

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Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Rejmontová

Capáková

Mikušová

et al. 2016

IJMS

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Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 53%

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Rejmontová

Capáková

Mikušová

et al. 2016

IJMS

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“…Preparation of ESc R1 line-derived cardiomyocytes from the HG8 clone were described previously 19 , 20 . Purified cardiomyocytes were seeded onto reference tissue culture dishes and onto all tested polyaniline surfaces.…”

Section: Experimental Sectionsmentioning

confidence: 99%

“…Neural progenitors were isolated from ganglionic eminences of embryonic brain (13.5 dpc) 19 , 21 and expanding in neurobasal media DMEM:F12 with B27 and N2 supplements, 100 U mL −1 penicillin, 0.1 mg mL −1 streptomycin (Gibco-Invitrogen; USA), 5 ng mL −1 of FGF-2 and 20 ng mL −1 of EGF (PeproTech; USA). Only cells up to passage 3 were used.…”

Section: Experimental Sectionsmentioning

confidence: 99%

Polyaniline cryogels: Biocompatibility of novel conducting macroporous material

Humpolíček

Radaszkiewicz

Capáková

et al. 2018

Sci Rep

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Polyaniline cryogel is a new unique form of polyaniline combining intrinsic electrical conductivity and the material properties of hydrogels. It is prepared by the polymerization of aniline in frozen poly(vinyl alcohol) solutions. The biocompatibility of macroporous polyaniline cryogel was demonstrated by testing its cytotoxicity on mouse embryonic fibroblasts and via the test of embryotoxicity based on the formation of beating foci within spontaneous differentiating embryonic stem cells. Good biocompatibility was related to low contents of low-molecular-weight impurities in polyaniline cryogel, which was confirmed by liquid chromatography. The adhesion and growth of embryonic stem cells, embryoid bodies, cardiomyocytes, and neural progenitors prove that polyaniline cryogel has the potential to be used as a carrier for cells in tissue engineering or bio-sensing. The surface energy as well as the elasticity and porosity of cryogel mimic tissue properties. Polyaniline cryogel can therefore be applied in bio-sensing or regenerative medicine in general, and mainly in the tissue engineering of electrically excitable tissues.

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“…10 Thanks to their easy handling, also using their self-assembling properties, the memristive polymers are the best candidates to obtain memory switching devices able to interface with biological materials. 11 Implementation of the functional properties of living systems into electronic memristive devices has recently obtained with the use of the slime mold PhysarumPolycephalum, a multinuclear unicellular mass of protoplasm. 12,13 To optimize the use of memristive devices as bio-electronic interfaces, it is necessary to asses their biocompatibility measuring the functional viability of the cells adhering to them and moreover assuring their ability to collect the signal directly from the cells.…”

Section: Introductionmentioning

confidence: 99%

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

et al. 2016

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ARTICLES YOU MAY BE INTERESTED INPolysaccarides-based gels and solid-state electronic devices with memresistive properties: Synergy between polyaniline electrochemistry and biology AIP Advances 6, 111302 (2016) A living bio-hybrid system has been successfully implemented. It is constituted by neuroblastic cells, the SH-SY5Y human neuroblastoma cells, adhering to a poly-anyline (PANI) a semiconductor polymer with memristive properties. By a multidisciplinary approach, the biocompatibility of the substrate has been analyzed and the functionality of the adhering cells has been investigated. We found that the PANI films can support the cell adhesion. Moreover, the SH-SY5Y cells were successfully differentiated into neuron-like cells for in vitro applications demonstrating that PANI can also promote cell differentiation. In order to deeply characterize the modifications of the bio-functionality induced by the cell-substrate interaction, the functional properties of the cells have been characterized by electrophysiology and Raman spectroscopy. Our results confirm that the PANI films do not strongly affect the general properties of the cells, ensuring their viability without toxic effects on their physiology. Ascribed to the adhesion process, however, a slight increase of the markers of the cell suffering has been evidenced by Raman spectroscopy and accordingly the electrophysiology shows a reduction at positive stimulations in the cells excitability. © 2016 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license

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Stem cell differentiation on conducting polyaniline

Cited by 37 publications

References 37 publications

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Polyaniline cryogels: Biocompatibility of novel conducting macroporous material

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

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