Stem cells and reproduction

Lee, Yeonmi; Kang, Eunju

doi:10.5483/bmbrep.2019.52.8.141

Cited by 8 publications

(4 citation statements)

References 79 publications

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“…Moreover, 3.5% of women with infertility who undergo assisted reproductive technology (ART) have no oocytes upon retrieval 27 . To date, none of the available techniques can be applied to these women for getting their babies 28 . Combined with the generation of artificial oocytes from pluripotent stem cells 29 , 30 , our technology can apply next-generation ART, including mitochondrial replacement therapy in which somatic cells from patients with infertility or mitochondrial disease could be introduced into the cytoplasm of enucleated artificial oocytes.…”

Section: Discussionmentioning

confidence: 99%

Haploidy in somatic cells is induced by mature oocytes in mice

et al. 2022

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Haploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy in somatic cells have not been successful. Here, we demonstrate that the replacement of meiotic spindles in mature metaphases II (MII) arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle results in the formation of de novo spindles consisting of somatic homologous chromosomes comprising of single chromatids. Fertilization of such oocytes with sperm triggers the extrusion of one set of homologous chromosomes into the pseudo-polar body (PPB), resulting in a zygote with haploid somatic and sperm pronuclei (PN). Upon culture, 18% of somatic-sperm zygotes reach the blastocyst stage, and 16% of them possess heterozygous diploid genomes consisting of somatic haploid and sperm homologs across all chromosomes. We also generate embryonic stem cells and live offspring from somatic-sperm embryos. Our finding may offer an alternative strategy for generating oocytes carrying somatic genomes.

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Section: Discussionmentioning

confidence: 99%

Haploidy in somatic cells is induced by mature oocytes in mice

et al. 2022

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“…Research shows that there is a clear difference between the quantity, distribution, and morphology of cytoplasmic organelles between poor-quality and good-quality oocytes [ 161 ]. Methods have been used to correct this defect in oocytes, including nuclear transfer, spindle transfer, polar body transfer, and pronuclear transfer [ 162 , 163 ].…”

Section: Discussionmentioning

confidence: 99%

Artificial Oocyte: Development and Potential Application

Oqani

Lee

et al. 2022

Cells

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Millions of people around the world suffer from infertility, with the number of infertile couples and individuals increasing every year. Assisted reproductive technologies (ART) have been widely developed in recent years; however, some patients are unable to benefit from these technologies due to their lack of functional germ cells. Therefore, the development of alternative methods seems necessary. One of these methods is to create artificial oocytes. Oocytes can be generated in vitro from the ovary, fetal gonad, germline stem cells (GSCs), ovarian stem cells, or pluripotent stem cells (PSCs). This approach has raised new hopes in both basic research and medical applications. In this article, we looked at the principle of oocyte development, the landmark studies that enhanced our understanding of the cellular and molecular mechanisms that govern oogenesis in vivo, as well as the mechanisms underlying in vitro generation of functional oocytes from different sources of mouse and human stem cells. In addition, we introduced next-generation ART using somatic cells with artificial oocytes. Finally, we provided an overview of the reproductive application of in vitro oogenesis and its use in human fertility.

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“…describe in their paper, there are several kinds of stem cells. Table 1 summarizes and succinctly elaborates on the stem cells used in infertility treatments, their distinctive traits and current therapeutic applications in reproductive medicine: [12,13], while the main drawback is genetic instability is [14].…”

Section: Stem Cells Variantsmentioning

confidence: 99%

Stem Cells and Infertility: A Review of Clinical Applications and Legal Frameworks

Cucinella,

Gullo,

Catania

et al. 2023

Preprint

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Infertility” is a condition defined by the failure to establish a clinical pregnancy after 12 months of regular, unprotected sexual intercourse or due to an impairment of a person’s capacity to reproduce either as an individual or with his/her partner’. The authors have set out to succinctly investigate, explore and assess infertility treatments harnessing the potential of stem cells to effectively and safely treat infertility, in addition to the legal and regulatory complexities at the heart of stem cell research, with an overview of the legislative state of affairs in six major European countries. In couples who cannot benefit from assisted reproductive technologies (ART) to treat their infertility, stem cells-based approaches have been shown to be a highly promising approach. Nonetheless, lingering ethical and immunological uncertainties require more conclusive findings and data before such treatment avenues can become mainstream and applied large scale. The isolation of human embryonic stem cells (ESCs) is ethically controversial, since their collection involves the destruction of human embryonic tissue. Overall, stem cell research has resulted in important new breakthroughs in the treatment of infertility. The effort to untangle the complex web of ethical and legal issues associated with such therapeutic approaches will have to rely on evidence-based, broadly shared standards, guidelines and best practices to make sure that the procreative rights of patients can be effectively reconciled with the core values at the heart of medical ethics.

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Stem cells and reproduction

Cited by 8 publications

References 79 publications

Haploidy in somatic cells is induced by mature oocytes in mice

Haploidy in somatic cells is induced by mature oocytes in mice

Artificial Oocyte: Development and Potential Application

Stem Cells and Infertility: A Review of Clinical Applications and Legal Frameworks

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