Stem extract of Tabebuia chrysantha induces apoptosis by targeting sEGFR in Ehrlich Ascites Carcinoma

Panda, Siva Prasad; Panigrahy, Uttam Prasad; Panda, Subhranshu; Jena, Bikash Ranjan

doi:10.1016/j.jep.2019.02.023

Cited by 14 publications

(11 citation statements)

References 31 publications

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“…The HPLC retention time for three compounds was 3.096, 2.784 and 3.691, respectively (Figure 1). [ 9 ] The structure of compound 4 named 4a,5,8,8a‐tetrahydro‐5‐hydroxy‐3,7,8‐trimethoxy‐2‐(3,4‐dimethoxyphenyl)chromen‐4‐one (TMF) was characterised by using UV, HPLC, IR, 1 H NMR and 13 C NMR spectrum (Figures and 2). The HPLC retention time of TMF was 2.460 min (Figure 3), and IR data were observed as OH: 3374.31 cm −1 , OMe: 2923.07 cm −1 , C=O: 1711.80 cm −1 (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…The bands of all proteomics were detected using the available software (LI‐COR Biosciences, Lincoln, NE, USA). [ 9 ]…”

Section: Methodsmentioning

confidence: 99%

“…Nicholson (Bignoniaceae) was 118.4 mg QE/g of the extract. [ 9 ] According to their chemical structure, flavonoids are divided into four categories: flavones, flavanones, flavonols and anthocyanin. The 16 species of plants belonging to the Bignoniaceae family are undergone phytochemical analysis and reported a large predominance of flavones over flavonols.…”

Section: Introductionmentioning

confidence: 99%

“…Our previous research demonstrated the apoptosis of EAT induced cancer tissue was due to suppression of sEGFR mediated pSTAT3 proteins. [ 9 ] Agents that target sEGFR potentially exert tumour‐suppressive effect by reducing the secretion of proangiogenic protein factor VEGF which stimulates tumour neoangiogenesis. [ 12,13 ] Some tumours may resistant to sEGFR targeted drugs and use alternative VEGF mediated STAT3‐MMP9 signalling for proliferation and angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

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A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma

Panda

Panigrahy

Prasanth

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a-tetrahydro-5-hydroxy-3,7,8-trimethoxy-2-(3,4-dimethoxyphenyl) chromen-4-one (TMF) from Tabebuia chrysantha. STAT3-MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in various cancers. Methods The tumour xenografting chicken embryo chorioallantoic membrane (CAM) model-based ex vivo assay was used to evaluate the activity of TMF. The Western blot, densitometric analysis and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the activity of the MMP9. Zebrafish embryos were used to evaluate embryotoxicity, and in vitro free radical scavenging activity of flavonoid was also elucidated. Key findings This research assessed the high level of STAT3, p-ERK, VEGF-R and MMP9 in the tissue extract of the control group, and also, the suppression of angiogenesis in the treatment groups was due to scavenged ROS and RNS, dephosphorylation of STAT3 and ERK, and suppression of MMP9 gene expression. Conclusion The isolated flavonoid named TMF from T. chrysantha functions as specific regulators of target proteins of angiosarcoma. The STAT3-MMP9 signalling may be used as an effective prognostic marker of angiosarcoma.

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Section: Resultsmentioning

confidence: 99%

“…The bands of all proteomics were detected using the available software (LI‐COR Biosciences, Lincoln, NE, USA). [ 9 ]…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma

Panda

Panigrahy

Prasanth

et al. 2020

Journal of Pharmacy and Pharmacology

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“…Two species belonging to the Tabebuia genus have documented anti-cancer activity. The anti-cancer effects of T. avellanedae and T. chrisantha are documented in preclinical xeno-transplant models (13) and in mice carrying Ehrilch ascites tumor (24). The use of TA in traditional medicine is not well documented.…”

Section: Discussionmentioning

confidence: 99%

Growth inhibitory efficacy and anti‑aromatase activity of Tabebuia avellanedae in a model for post‑menopausal Luminal A breast cancer

Telang¹,

Nair

Wong³

2019

biom rep

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Aromatase inhibitors (AIs) represent a treatment option for post-menopausal estrogen receptor-positive (ER +) breast cancer as monotherapy, or in combination with cyclin-dependent kinase 4/6 or mTOR inhibitors. Long-term treatment with these agents leads to dose-limiting toxicity and drug resistance. Natural substances provide testable alternatives to current therapy. Tabebuia avellanedae (TA) tree is indigenous to the Amazon rainforest. The inner bark of TA represents a medicinal dietary supplement known as Taheebo. Non-fractionated aqueous extract from TA is an effective growth inhibitor in the Luminal A and triple negative breast cancer models. The quinone derivative naphthofurandione (NFD) is a major bioactive agent in TA. The present study examined the efficacy of finely ground powder from the inner bark of TA, available under the name of Taheebo-NFD-Marugoto (TNM). The ER + MCF-7 cells stably transfected with the aromatase gene MCF-7 AROM represented a model for aromatase-expressing post-menopausal breast cancer. Anchorage-independent colony formation, cell cycle progression, pro-apoptotic caspase 3/7 activity, apoptosis-specific gene expression, aromatase activity and select estradiol (E 2) target gene expression represented the mechanistic end points. Treatment of MCF-7 AROM cells with TNM induced a dose-dependent reduction in E 2-promoted anchorage-independent colony number. Mechanistic assays on TNM-treated MCF-7 AROM cells demonstrated that TNM at a concentration of 10 µg (NFD content: 2 ng), induced S-phase arrest, increased pro-apoptotic caspase 3/7 activity, increased pro-apoptotic BAX and decreased anti-apoptotic BCL-2 gene expression, and inhibited aromatase activity. Additionally, TNM treatment downregulated ESR-1 (gene for ER-α), aromatase and progesterone gene expression and reduced mRNA levels of E 2 target genes pS2, GRB2 and cyclin D1. Inhibition of aromatase activity, based on the NFD content of TNM was superior to the clinical AIs Letrozole and Exemestane. These data demonstrated the potential efficacy of TNM as a nutritional alternative for current therapy of aromatase positive, post-menopausal breast cancer.

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Evaluation of the Active Ingredient of Campsis radicans Essential Oils and its Antimicrobial Evaluation Against Pathogenic Bacteria

et al. 2022

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Stem extract of Tabebuia chrysantha induces apoptosis by targeting sEGFR in Ehrlich Ascites Carcinoma

Cited by 14 publications

References 31 publications

A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma

A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma

Growth inhibitory efficacy and anti‑aromatase activity of Tabebuia avellanedae in a model for post‑menopausal Luminal A breast cancer

Evaluation of the Active Ingredient of Campsis radicans Essential Oils and its Antimicrobial Evaluation Against Pathogenic Bacteria

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