2019
DOI: 10.1002/jbio.201960090
|View full text |Cite
|
Sign up to set email alerts
|

Step‐by‐step monitoring of a magnetic and SERS‐active immunosensor assembly for purification and detection of tau protein

Abstract: We report a bottom‐up synthesis of iron oxide and gold nanoparticles, which are functionalized and combined to form a nanohybrid serving as an immune sensor, which selectively binds to tau protein, a biomarker for diagnosis of Alzheimer's disease. Detection of the target analyte is achieved by surface‐enhanced Raman scattering originating from the diagnostic part of the nanohybrid that was prepared from Au nanoparticles functionalized with 5,5′‐dithiobis‐(2‐nitrobenzoic acid) as a Raman reporter and monoclonal… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
19
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
6
1

Relationship

1
6

Authors

Journals

citations
Cited by 19 publications
(19 citation statements)
references
References 36 publications
(38 reference statements)
0
19
0
Order By: Relevance
“…As a further confirmation that the magnetic‐plasmonic dots act as enhancers for the Raman scattering of the MG molecules, the bidimensional intensity map of the MG peak at 1615 cm −1 was collected on six dots loaded with 20 pmol of dye using the 532 nm excitation laser line. There are no other examples in the literature demonstrating SERS with Au−Co alloy nanoparticles but the sensitivity in terms of the amount of analyte detected is comparable or superior to that of other bimetallic Au−Fe [11,44] or Ag−Fe NPs [12,34] …”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…As a further confirmation that the magnetic‐plasmonic dots act as enhancers for the Raman scattering of the MG molecules, the bidimensional intensity map of the MG peak at 1615 cm −1 was collected on six dots loaded with 20 pmol of dye using the 532 nm excitation laser line. There are no other examples in the literature demonstrating SERS with Au−Co alloy nanoparticles but the sensitivity in terms of the amount of analyte detected is comparable or superior to that of other bimetallic Au−Fe [11,44] or Ag−Fe NPs [12,34] …”
Section: Resultsmentioning
confidence: 99%
“…Bimetallic nanoparticles containing plasmonic and transition metals are promising for surface‐enhanced Raman scattering (SERS) of important biomolecules such as serotonin [10] . Magnetic‐plasmonic bimetallic systems have the ability to magnetically capture target analytes and separate them from the assay matrix, that is crucial for the detection of disease‐specific biomarkers in body fluids by spectroscopic methods such as SERS [11,12] . Pt alloy NPs are intensely investigated for their oxygen reduction reaction catalytic performances [13,14] .…”
Section: Introductionmentioning
confidence: 99%
“…If entities with strong magnetic properties (e.g., superparamagnetic iron oxide nanoparticles, referred to as Fe x O y -NPs) are entrapped, an external magnetic field can be used to achieve a magnetic targeting functionality of the resulting hybrid niosomes [ 49 , 50 ]. Thus, the superparamagnetic structures can be guided to and accumulate at the intended target tissue which consequently lowers concentrations in off-target sites and, hence, significantly improves the therapeutic outcome [ 51 , 52 , 53 , 54 ].…”
Section: Introductionmentioning
confidence: 99%
“…In comparison to Zengin et al, Maurer et al reported a sandwich assay to detect tau in CSF, simplifying the magnetic component by Fe x O y NPs functionalized with polyclonal anti-tau antibody ( Figure 4 C). This approach reduced synthesis time and the workload for preparing the whole tau protein-specific immunosensor and can be transferred to other protein biomarkers, by exchanging the specific antibody [ 81 ].…”
Section: Plasmonic Biosensors For In Vitro Optical Detection Of Admentioning
confidence: 99%
“…Reproduced with permission of Wiley Materials from ref. [ 81 ] ( D ) Graphical scheme of 3D SERS-substrate for sensitive Aβ and tau detection. Adapted with permission from ref.…”
Section: Figurementioning
confidence: 99%