Stephania delavayi Diels. inhibits breast carcinoma proliferation through the p38 MAPK/NF-κB/COX-2 pathway

Park, Dae-Hun; Xu, Hao; Shim, Ju Hyun; Li, Yongchun; Lee, Jong-Hwa; Cho, Soon-Chang; Han, Sang-Seop; Lee, Yun Lyul; Lee, Min-Jae; Kwon, Sung Won

doi:10.3892/or.2011.1364

Cited by 3 publications

(5 citation statements)

References 27 publications

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“…These results indicated that matrine significantly decreased IKKβ expression in the treated breast cancer cells, suggesting that matrine effectively inhibited the proliferation of breast cancer cells by a mechanism associated with IKKβ (24). In conclusion, the present results suggested that matrine may be a promising reagent for treating breast cancer in the future, following further research.…”

Section: Discussionmentioning

confidence: 93%

Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF-κB signaling pathway

Shao

Yang

et al. 2013

Oncology Letters

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Matrine is an alkaloid isolated from Sophora flavescens. The present study aimed to determine whether matrine effectively inhibits the proliferation of breast cancer cells, and the underlying mechanism(s) of its antitumor function. The effects of matrine on the cell viability of ER-positive MCF7 cells, HER2-positive BT-474 cells and highly metastatic MDA-MB-231 cells were measured using MTT and apoptosis assays. Western blot analysis was performed to investigate the expression levels of the inhibitor of κB (IκB) kinase β (IKKβ) in cells treated with or without matrine. It was observed that the matrine treatment resulted in the death of the three types of cancer cells, but significantly less toxicity was observed in the control cancer cells. The experimental results also suggested that the antitumor effects of matrine on breast cancer cells may be associated with the downregulation of IKKβ expression by matrine, as indicated by the western blot analysis results. The present results suggested that matrine may be used as an effective drug candidate for treating breast cancers in the future, following further research.

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Section: Discussionmentioning

confidence: 93%

Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF-κB signaling pathway

Shao

Yang

et al. 2013

Oncology Letters

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“…There are several mechanisms to induce cell apoptosis including the caspase cascade, the Bcl family pathway ( 35 ), or the NF-κB-COX-2 pathway ( 36 ). In our previous study on S. delavayi Diels., the FK-3000 parental material demonstrated an anti-proliferative effect via the NF-κB-COX-2 pathway ( 26 ). Therefore, we investigated whether FK-3000 could induce apoptosis in the same manner using MDA-MB-231 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we reported that S. delavayi Diels. suppressed MDA-MB-231 carcinoma proliferation by inducing apoptosis ( 26 ). In this study FK-3000 and sinococuline were isolated from S. delavayi Diels.…”

Section: Discussionmentioning

confidence: 99%

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FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

Cho²,

Bang

et al. 2015

International Journal of Oncology

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The World Health Organization (WHO) has reported that cancer is one of the most prevalent diseases and a leading cause of death worldwide. Many anticancer drug development studies have been pursued over the last few decades and several viable drugs have been discovered, such as paclitaxel, topotecan and irinotecan. Previously, our research group uncovered the cytocidal and cytostatic effects of the plant Stephania delavayi Diels. In this study, we determined the active chemical to be 6,7-di-O-acetylsinococuline (FK-3000). The FK-3000 half maximal inhibitory concentration (IC50) in MDA-MB-231 breast carcinoma cells at 48 h was 0.52 μg/ml and it induced apoptosis in a dose- and time-dependent manner. FK-3000 suppressed NF-κB nuclear translocation, decreased NF-κB phosphorylation, and decreased COX-2 protein expression. MDA-MB-231 xenografted mice were treated with FK-3000, Taxol, or their combination for 21 days. The tumor size was smallest in the co-treatment group, indicating that FK-3000 may have a synergistic effect with Taxol. FK-3000 treatment showed no adverse effects on blood cell counts, serum protein levels, or pathology. These studies demonstrate that FK-3000, isolated from S. delavayi Diels., is a promising, pathway-specific anticancer agent that exhibits low toxicity.

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“…In 2014, the American Cancer Society reported that cancer was the second leading cause of death in the USA, estimating that >1,665,540 new cancer cases would be diagnosed that year and 585,720 cancer deaths would occur in the USA. To develop effective treatments, scientists have isolated anticancer agents from natural materials and identified a number of promising drug candidates ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation

Kim

Cho³

et al. 2014

International Journal of Oncology

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We evaluated the cytostatic effect of 6,7-di-O-acetylsinococuline (FK-3000) isolated from Stephania delavayi Diels. against breast carcinoma cell lines MDA-MB-231 and MCF-7. FK-3000 suppressed CDC25B phosphorylation directly and indirectly via p38 MAPK phosphorylation. CDC25B dephosphorylation decreased levels of cyclin B and phospho-CDC-2, and ultimately induced cell cycle arrest at the G2/M phase. The p38 MAPK inhibitor, SB 239063 blocked FK-3000-induced p38 MAPK phosphorylation and nuclear accumulation, but did not completely rescue cell death. Conclusively FK-3000 exerts its antiproliferative effect through two pathways: i) G2/M cell cycle arrest via downregulation of cyclin B and phospho-CDC2 by p38 MAPK phosphorylation and CDC25B dephosphorylation, and ii) p38 MAPK-independent induction of apoptosis.

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Stephania delavayi Diels. inhibits breast carcinoma proliferation through the p38 MAPK/NF-κB/COX-2 pathway

Cited by 3 publications

References 27 publications

Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF-κB signaling pathway

Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF-κB signaling pathway

FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

6,7-di-O-acetylsinococuline (FK-3000) induces G2/M phase arrest in breast carcinomas through p38 MAPK phosphorylation and CDC25B dephosphorylation

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