Stepwise dismantling of adenovirus 2 during entry into cells

Greber, Urs F.; Willetts, Melanie; Webster, Paul; Helenius, Ari

doi:10.1016/0092-8674(93)90382-z

Cited by 745 publications

(782 citation statements)

References 72 publications

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“…The adenovirus internalization pathway, which begins with binding of adenovirus to the coxsackie and adenovirus receptor, 25 has been well studied and characterized. 26,27 Moreover, microarray technology has been minimally exploited with respect to the study of adenovirus-host cell interaction. [10][11][12][13][14] Therefore, further investigations of adenoviral infection may reveal unique changes in gene expression that induced at different points in its infection pathway.…”

Section: Resultsmentioning

confidence: 99%

Employment of microarray analysis to characterize biologic differences associated with tropism-modified adenoviral vectors: utilization of non-native cellular entry pathways

et al. 2004

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In this study, we have applied high-density oligonucleotide microarray technology to characterize biologic changes associated with adenoviral vector-mediated target cell infection. We infected a human melanoma cell line, M21, with the tropism-modified vectors, Ad5lucRGD and Ad5/3luc1. In addition, we infected the M21 cell line with the Ad5luc1, a vector which primarily exploits the coxsackie and adenovirus receptor, as its primary native receptor. We found significant changes in gene expression of 5492 genes induced by Ad5luc1 infection, 2439 genes induced by Ad5/3luc1 infection, and 1251 genes induced by Ad5lucRGD infection, compared to unifected cells. Among these changes in gene expression, 783 changes were common to Ad5/3luc1 and Ad5luc1 infections, 266 were common to Ad5lucRGD and Ad5luc1 infections, and 185 changes in gene expression were common to Ad5/3luc1 and Ad5lucRGD infections. Interestingly, 89 changes in gene expression were common to all the three groups, suggesting a commonly affected pathway. This analysis represents a unique application of microarray to study vector-related issues.Furthermore, these studies demonstrate the utility of microarray for characterizing the biologic sequelae of host-vector interaction.

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Section: Resultsmentioning

confidence: 99%

Employment of microarray analysis to characterize biologic differences associated with tropism-modified adenoviral vectors: utilization of non-native cellular entry pathways

et al. 2004

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“…24,25 In contrast, lentiviral and adenovirus-based gene delivery systems have been shown to transduce non-proliferating cells. [15][16][17]26 Reports describing the role of cell division in cationic lipid-based transfection systems have only recently begun to appear in the literature. Wilke et al 11 showed that LLC-PK1 cells that were prevented from proliferating by growth in serum-free media were less efficiently transfected with lipofectin (DOTMA:DOPE) than control proliferating cells grown in media containing serum.…”

Section: Figure 7 Semliki Forest Virus-mediated-gene Expression Sk-omentioning

confidence: 99%

Cationic lipid-mediated transfection of cells in culture requires mitotic activity

et al. 1999

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Cationic lipid-based delivery systems such as lipoplexes or aphidicolin exhibited 20-fold lower reporter gene activity stabilized plasmid-lipid particles (SPLP) represent a safer than asynchronous control cells upon incubation with lipoalternative to viral systems for gene therapy applications.plexes. When cells arrested in the G1 phase were allowed We studied the impact of cell cycle status on the efficiency to proceed though the cell cycle in the presence of the lipoof transfection of human ovarian carcinoma tumor cells plex or SPLP, transgene expression was found to coincide using two cationic-lipid based delivery systems. Cells with the transition of cells from the G2/M phase into the arrested in the G1 phase of the cell cycle by treatment with G1 phase of the subsequent cell cycle. In addition, higher aphidicolin were compared with an asynchronous dividing levels of reporter gene expression were observed when the population of cells. Treatment of the cells with aphidicolin cells were incubated with lipoplexes or SPLP during, or just had no effect on the rate of internalization of the lipid forbefore, mitosis. These results suggest that it may be possmulated DNA or on the level of gene expression observible to augment cationic lipid-mediated transfection by able in stably transfected cells. However, cells treated with manipulating the cell cycle status of the target cells.

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“…16 These processes may assist release of viral DNA, transport of DNA to the nuclear pore complex, and finally transit through the pore into the nucleus. 17,18 The importance of the interaction between adenovirus penton base and cell surface ␣v integrin has been shown during infection of M21, CS-1, A549, HeLa and THP-1 cell lines. 13,14,16,19,20 For example, M21 cells expressing ␣v integrins were more readily infected by adenovirus than were ␣v integrin-negative M21 cells.…”

Section: Introductionmentioning

confidence: 99%

An interaction between penton base and αv integrins plays a minimal role in adenovirus-mediated gene transfer to hepatocytes in vitro and in vivo

et al. 1998

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Studies in cultured cell lines have shown that adenovirus tion of hepatocytes, even though the mutation impaired infection involves binding of adenovirus fiber to its cell surinfection of HeLa cells. Hepatocytes had undetectable face receptor and binding of penton base to ␣v integrins.amounts of ␣v integrins on their cell surface and showed However, much less is known about the role of these interno specific adherence to vitronectin, the natural substrate actions in cells that are targets for adenovirus-mediated of ␣v integrins. Adenovirus with an intact penton base gene transfer. Earlier work showed that hepatocytes are enhanced infection of liver following intravenous injection, readily infected by adenovirus, making them an attractive but only by three-fold as compared with virus in which the target for gene therapy in several diseases. We found that integrin-binding motif was disrupted. These studies sugaddition of fiber protein blocked adenovirus infection of prigest that interactions between cell surface integrins and mary cultures of hepatocytes. This suggests an important penton base are not required for adenovirus infection of role for fiber and its receptor. However, mutation of the hepatocytes in vitro, but the interaction enhances infection integrin-binding motif in penton base did not inhibit infecto a small degree in vivo.

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Stepwise dismantling of adenovirus 2 during entry into cells

Cited by 745 publications

References 72 publications

Employment of microarray analysis to characterize biologic differences associated with tropism-modified adenoviral vectors: utilization of non-native cellular entry pathways

Employment of microarray analysis to characterize biologic differences associated with tropism-modified adenoviral vectors: utilization of non-native cellular entry pathways

Cationic lipid-mediated transfection of cells in culture requires mitotic activity

An interaction between penton base and αv integrins plays a minimal role in adenovirus-mediated gene transfer to hepatocytes in vitro and in vivo

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