Stereological comparison of oocyte recruitment and batch fecundity estimates from paraffin and resin sections using spawning albacore (Thunnus alalunga) ovaries as a case study

Trade‐offs between life‐history traits in a coldwater fish in the Mediterranean Sea: the case of blue whiting Micromesistius poutassou

“…Diameter corrected for shrinkage, applying correction factors given for paraffin‐embedded material (Saber et al, ).…”

Reproductive strategies of the marine fishes from the southwest Atlantic Ocean: an application of histological and image processing techniques

“…According to Ganias (2013), V i of the standing stock of vitellogenic oocytes should be high than those in pre-vitellogenesis. In general, this pattern was observed in this study, also corroborating the results found by Saber et al (2014) in Thunnus alalunga. These authors have reported values of V i (previtellogenic and vitellogenic oocytes) less than 50% and described the relationship between OD vi and V i by a polynomial function, as recorded in both species here.…”

“…Korta et al (2010b) and Saber et al (2014) have reported similar result for fishes with indeterminate fecundity. Therefore, the asynchronous oogenesis with no gap between the pre-vitellogenic and vitellogenic modes in the oocyte size-frequency distribution are associated with multiple spawning fishes, and this represents a strategy for investment of large numbers of oocytes when resources are patchy (WINEMILLER;ROSE, 1993;.…”

“…The ovaries containing the most development stages such as germinal vesicle migration and hydrated oocytes were not analysed in this study due to their shrinkage in the histological sections, which could affect the final result overestimating the stereological variables, e.g. the volume fraction, as observed by Schismenou et al (2012) and Saber et al (2014). The descriptions of each oocyte stage are detailed in Table Oocyte production and recruitment pattern of two congeneric species | 168 Table 2.…”

“…Stereological methods have been successful applied in fishery science for estimation of the number of pre-vitellogenic and vitellogenic oocytes, so-called developing oocytes (NDO), in fishes with indeterminate and determinate fecundity (COWARD;BROMAGE, 2002;DOMÍNUEZ-PETIT, 2007;MEDINA et al, 2007;ARAGÓN et al, 2010;DOMÍNUEZ-PETIT;SABORIDO-REY, 2010;HASLOB et al, 2013;HERNÁNDEZ-PORTOCARRERO et al, 2014;GARCÍA-SEOANE et al, 2014;KNAPP et al, 2014;SABER et al, 2014). Differences in the final output among species often reflect different reproductive strategies, although the final oocyte production of a given species may vary in response to changeable environment and biological factors (WOOTON, 1984;WITTHAMES et al, 1995) HELFMAN et al, 1997.…”