Stereological Evidences of Epithelial Hypoplasia of Seminiferous Tubules Induced By Mesterolone in Adult Sprague-Dawley Rats

Abstract

BACKGROUND: Anabolic-androgenic steroid compounds are one of the most widely abused drugs by athletes and muscle builders with the goal of improving performance/ability, appearance, or muscle mass. In addition, these steroids are widely used in the treatment of male infertility and subfertility. However, increasing concern has been shown that these compounds may not only offer unappreciable benefits to infertile and subfertile males, but might have deleterious effects on both human and animal physiology and sperm quality. There is a dearth of knowledge on the structural and quantitative changes of the testis secondary to this group of compounds. Objective: The present study was carried out to evaluate the effects of mesterolone (proviron), an anabolic-androgenic steroid, on some of the histomorphometric and stereological parameters of the seminiferous tubules in Sprague-Dawley rat. Materials and Methods: Two groups of 10 adult male rats were used. The treated group was given 0.06 mg/kg body weight/day of mesterolone by gavage for six weeks while the control group received equal volume of 0.9% normal saline per day. Five µm of uniformly random serial sections of the processed testicular tissues were analyzed using un-biased stereological and histomorphometric studies. Results:The results showed that the percentage mean volume density of both the tubular lumen and epithelial height increased by 35% (p< 0.05) and decreased by 50% (p<0.05), respectively compared to the control. mesterolone also caused a significant decline in sperm concentration. Conclusion: Mesterolone produces epithelial hypoplasia in the testis post continuous management.

The effects of sesame oil and different doses of estradiol on testicular structure, sperm parameters, and chromatin integrity in old mice

“…Furthermore, changes in tubal diameter could occur due to an increase in the number of germ lineage cells within the seminiferous tubules [ 51 ]. According to Shittu et al [ 52 ], sesame oil raises testosterone levels and testosterone increases spermatogenesis in male animals. The aqueous extract of sesame leaves has an antioxidant effect and significantly increases the number of spermatogonia, seminiferous tubules, and testosterone levels.…”

“…The aqueous extract of sesame leaves has an antioxidant effect and significantly increases the number of spermatogonia, seminiferous tubules, and testosterone levels. According to other researchers, sesame phytoestrogens bind to testicular estrogen receptors and stimulate spermatogenesis through the proliferation of epithelial cells and sex cells [ 52 , 53 ]. In the sesame oil and high-dose estradiol groups, a higher rate of chromatin and DNA damage was observed than in the control group and the low-dose estradiol group.…”

Ex-situ Morphological and Morphometric Studies of the Testis In the West African Dwarf Buck GoatEx-situ Morphological and Morphometric Studies of the Testis In the West African Dwarf Buck Goat

“…These differences may be due to breed variation. However, relating these with previous documentations that spermatogenic activities within the seminiferous tubules is a function of GEH; the higher the GEH, the more active the seminiferous tubule (in terms of spermatogenesis) and vice-versa (Castro et al, 2002;Shittu et al, 2008); the findings of this present study, suggest high spermatogenic activities in the seminiferous tubules of the WAD buck goat.…”

Increase in Bcl2 expression of penile and prostate cells of Sprague Dawley male rats following treatment with buceng (combination of Pimpinella alpina molk with Eurycoma longifolia Jack)

“…17 In this study, the dose of mesterolone was 6.75 mg/day or equivalent to 0.02 -0.06 mg/kg BW/day, based on the physiological calculation for 70 kg man. 18 Higher or supraphysiologic dose of mesterolone will result in intoxication indicated by weak, sluggish, even unmoved rats.…”