Stereoscopic Effects from Single SEM Images

Sturm, Robert

doi:10.1017/s1551929518000640

Cited by 4 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the specimen was tilted along a vertical axis (i.e., perpendicular to the axis joining both eyes of the observer) at 5° and/or 8°, and a second SEM image was recorded at the same magnification and WD. 17,18 Both SEM images were later merged and read into the xT microscope Control software (FEI, Hillsboro, OR, USA), turned into red and cyan images, and then overlapped using the "stereo pair" option. Figures 4 and S2 in the Supplementary Material show the red-cyan anaglyphs of different cavolinioidean species with sufficient parallax effects.…”

Section: Three-dimensional Images (Anaglyphs)mentioning

confidence: 99%

Evidence for helical microstructure of aragonite fibers in pteropod shells

et al. 2022

View full text Add to dashboard Cite

Planktonic gastropods of the suborder Euthecosomata (order Pteropoda) secrete a unique microstructure made of tightly interlocked, space-filling helically coiled aragonite fibers. This material has a high degree of ordering because all fibers are in phase at a given growth plane. The recognition of the helical nature from mere scanning electron micrographs is somewhat uncertain. In addition, three-dimensional (3D) reconstructions of individual helices are hindered by the small dimensions of fibers, and the virtual absence of intervening organic interphases. Here, we (1) reexamine the morphological characteristics of the fibers in fracture and find them compatible with a helical model, (2) provide direct evidence of the helical mode of coiling in the form of anaglyphs, and (3) develop a 3D model, which is fully consistent with the patterns observed in fractures and polished sections. Proposals based on motifs different from helices are not congruent with the observations. Graphical abstract Impact statement Some planktonic gastropods of the order Pteropoda secrete a unique microstructure that was originally described as made of tightly interlocked helically coiled continuous aragonite fibers. Despite this material having been described 50 years ago, there is still debate as to the helical design of such fibers. We provide additional unambiguous evidence that fibers describe true helical trajectories during growth. Establishing their true nature is essential in order to know the spectrum of calcified biomaterials produced by molluscs. This is also a prerequisite for future biomimetic studies aiming at producing bioinspired highly functional materials.

show abstract

Section: Three-dimensional Images (Anaglyphs)mentioning

confidence: 99%

Evidence for helical microstructure of aragonite fibers in pteropod shells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Two recent articles in Microscopy Today describe methods that purport to produce three-dimensional (3D) information from single SEM images [1][2]. They are bogus.…”

mentioning

confidence: 99%