Stereoselective determination of unchanged and glucuroconjugated eliprodil, a new anti-ischaemic drug, in human plasma and urine by precolumn derivatization and column-switching high-performance liquid chromatography with fluorescence detection

Effect of a neuroprotective drug, eliprodil on cardiac repolarisation: importance of the decreased repolarisation reserve in the development of proarrhythmic risk

“…At this concentration, BaCl 2 does not affect I Kr , I Ks or I to but depresses I K1 by about 60% ( Biliczki et al ., 2002 ). Eliprodil, which has an estimated effective plasma concentration ( Garrigou‐Gadenne et al ., 1995 ; Malavasi et al ., 1996 ) around the micromolar range and of whose effect on cardiac potassium channels has not been characterised so far, did not influence potassium currents other than I Kr in our experimental conditions. Therefore, the present results extend our previous observation that simultaneous blockade of different potassium channels decreases the ‘repolarisation reserve’ and enhances APD prolongation ( Biliczki et al ., 2002 ).…”

On-Line Coupling of Solid Phase Extraction Sample Processing with High-Performance Liquid Chromatography

“…The sample is then purified on a SPE precolumn and finally transferred by the mobile phase to the analytical column. [37][38][39][40][41] Other assays are based on the use of reagents attached to solid supports. 42,43 These materials effect sample clean-up and at the same time, or somewhat later, the reagent part of material is made to react with the analyte.…”

“…Immunosorbents and molecular imprinted materials, which are based on molecular recognition, also allow achieving isolation and preconcentration in a single step. On-line SPE sample processing is very well suited for analysis of biological fluids such as urine [7][8][9][10]15,[20][21][22][23][25][26][27]30,31,37,39,[44][45][46]59,[94][95][96][97][98][99]102 and plasma. 7,8,15,16,18,20,22,33,36,37,56,57,69,[70][71][72][73]94,96,100 The determination of various pharmaceutics and drugs of this kind of matrices is required for pharmacokinetic and bioavailability studies 7,8,15,16, 30,38, 45,56, …”

Role of biological matrices during the analysis of chiral drugs by liquid chromatography

“…The on-line achiral-chiral systems were developed for the determinations of metoprolol in human urine [130]; verapamil and its metabolites in serum [104]; tramadol enantiomers in human urine [105]; verapamil in human plasma [131]; eliprodil in human plasma and urine [132]. Two different types of CSW set-up (single-pump system with ovomucoid CSP as well as two-pumps set-up with teicoplanin CSP) were compared for determination of propranolol enantiomers in rat blood microdialysate [59].…”