Sterilization mechanism for <i>Escherichia coli</i> by plasma flow at atmospheric pressure

Sato, Takehiko; Mutoh, T.; Doi, Akiko; Ochiai, Shiroh; Urayama, Takuya; Nakatani, Tatsuyuki

doi:10.1063/1.2336594

Cited by 85 publications

(59 citation statements)

References 6 publications

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“…Indeed encouraged by their potential as a bactericidal technique, 2 low-temperature gas discharges have been studied extensively for bacterial inactivation both in a vacuum chamber at low pressures [3][4][5][6] and in open air at atmospheric pressure. [7][8][9][10][11][12] Much has been learnt of the bactericidal capability and related physical mechanisms of low-temperature plasmas. 13 By contrast, there are very few reports on their ability to destruct proteinaceous matters [14][15][16] and all reported are limited to the use of vacuum plasmas.…”

mentioning

confidence: 99%

Protein destruction by atmospheric pressure glow discharges

Deng

Shi

Chen

et al. 2007

Applied Physics Letters

161

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It is well established that atmospheric pressure glow discharges are capable of bacterial inactivation. Much less known is their ability to destruct infectious proteins, even though surgical instruments are often contaminated by both bacteria and proteinaceous matters. In this letter, the authors present a study of protein destruction using a low-temperature atmospheric dielectric-barrier discharge jet. Clear evidences of protein removal are presented with data of several complimentary experiments using scanning electron microscopy, electron dispersive x-ray analysis, electrophoresis, laser-induced fluorescence microscopy, and protein reduction kinetics. Considerable degradation is observed of protein fragments that remain on their substrate surface after plasma treatment.

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confidence: 99%

Protein destruction by atmospheric pressure glow discharges

Deng

Shi

Chen

et al. 2007

Applied Physics Letters

161

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“…E. coli seeded on an agar was disinfected within 20 seconds. Sato et al reported the disinfection efficacy of a coaxial microwave (2.45 GHz) Ar plasma flow at atmospheric pressure against E. coli filtered onto membrane filters [6]. They found that E. coli was disinfected by exposure to the plasma flow for 2 min.…”

Section: Resultsmentioning

confidence: 99%

“…It is considered that the short exposure distance is the reason for realizing a short exposure time in our method com- pared with conventional methods. For microwave-induced plasma, the exposure distance was as long as 124 mm [6]. It is considered that the distance is set long to suppress the disinfection by heat due to the high temperature of microwave-induced plasma.…”

Section: Resultsmentioning

confidence: 99%

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Scanning Electron Microscopy Image of Escherichia Coli Exposed with Atmospheric-Pressure Plasma Jet

Kuwahata

Kumazawa

Ohyama

et al. 2010

e-J. Surf. Sci. Nanotechnol.

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The morphological change of Escherichia coli (E. coli) exposed to an atmospheric-pressure argon (Ar) plasma jet was observed by scanning electron microscopy (SEM). The Ar plasma jet was generated at a frequency of 9 kHz, applied voltage of 10 kV, and Ar gas flow rate of 10 L/min at atmospheric pressure. E. coli seeded on an agar plate in a Petri dish was disinfected by Ar plasma jet exposure for 2 seconds. SEM images showed that E. coli cells deformed and faded after the Ar plasma jet exposure. The morphological changes were probably due to the destruction of the cell wall and cell membrane of E. coli caused by the collision of Ar ions and/or electrons in the Ar plasma jet.

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“…For sterilization, there are reports on the inactivation of bacteria, such as Escherichia coli [5,6], Micrococcus luteus [7], and Staphylococcus aureus [8], using atmospheric-pressure plasma and on the disruption of Bacillus subtilus spores [9,10] and Bacillus atrophaeus spores [11,12]. For medical applications, it was reported that the irradiation of mammalian cells with atmospheric-pressure plasma caused their necrosis [13] or apoptosis [14] depending on the irradiation conditions.…”

Section: Introductionmentioning

confidence: 99%

Two-Dimensional Concentration Distribution of Hydrogen Peroxide Generated by Atmospheric-Pressure Plasma Jet Irradiation

Kuwahata

Yamaguchi

2015

e-J. Surf. Sci. Nanotech.

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The two-dimensional concentration distribution of hydrogen peroxide (H2O2) generated by atmospheric-pressure argon (Ar) plasma jet irradiation was semiquantitatively determined using H2O2 test strips. An Ar plasma jet was generated with different applied voltages (5-10 kV) and Ar gas flow rates (3-10 L/min) and was irradiated onto the H2O2 test strips at different irradiation distances (2-40 mm) and irradiation times (5-30 s). The shape of the distribution of H2O2 concentration depended on the combination of the applied voltage, gas flow rate, and the irradiation distance and was classified into four types: circular, double-circular, ring-shaped, and double-ringshaped. A circular distribution was observed for irradiation distances of ≥30 mm and a double-circular distribution was observed for irradiation distances of 10-20 mm. When the irradiation distance was ≤5 mm, a ring-shaped distribution was observed for applied voltages of ≤6 kV and a double-ring-shaped distribution was observed for applied voltages of ≥7 kV.

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Sterilization mechanism for Escherichia coli by plasma flow at atmospheric pressure

Cited by 85 publications

References 6 publications

Protein destruction by atmospheric pressure glow discharges

Protein destruction by atmospheric pressure glow discharges

Scanning Electron Microscopy Image of Escherichia Coli Exposed with Atmospheric-Pressure Plasma Jet

Two-Dimensional Concentration Distribution of Hydrogen Peroxide Generated by Atmospheric-Pressure Plasma Jet Irradiation

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