Sterilization treatments on polysaccharides: Effects and side effects on pectin

Munarin, Fabiola; Bozzini, Sabrina; Visai, Livia; Tanzi, Maria Cristina; Petrini, Paola

doi:10.1016/j.foodhyd.2012.09.017

Cited by 51 publications

(41 citation statements)

References 44 publications

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“…42,43 This is in accordance with the SEC analysis performed, from which it could be concluded that the M w of PURpec is not different from the M w of RAWpec. The value determined for the M w of RAWpec by SEC is in agreement with the values previously reported in the literature 44,45 for the same Herbstreith & Fox Classic CU701 pectin (with similar GalA unit content and DM), despite being from different batches.…”

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confidence: 91%

Biofunctionalized pectin hydrogels as 3D cellular microenvironments

et al. 2015

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In situ-forming hydrogels of pectin, a polysaccharide present in the cell wall of higher plants, were prepared using an internal ionotropic gelation strategy based on calcium carbonate/D-glucono-d-lactone, and explored for the first time as cell delivery vehicles. Since no ultrapure pectins are commercially available yet, a simple and efficient purification method was established, effectively reducing the levels of proteins, polyphenols and endotoxins of the raw pectin. The purified pectin was then functionalized by carbodiimide chemistry with a cell-adhesive peptide (RGD). Its gelation was analyzed by rheometry and optimized. Human mesenchymal stem cells embedded within unmodified and RGD-pectin hydrogels of different viscoelasticities (1.5 and 2.5 wt%) remained viable and metabolically active for up to 14 days. On unmodified pectin hydrogels, cells remained isolated and round-shaped. In contrast, within RGD-pectin hydrogels they elongated, spread, established cell-to-cell contacts, produced extracellular matrix, and migrated outwards the hydrogels. After 7 days of subcutaneous implantation in mice, acellular pectin hydrogels were considerably degraded, particularly the 1.5 wt% hydrogels. Altogether, these findings show the great potential of pectin-based hydrogels, which combine an interesting set of easily tunable properties, including the in vivo degradation profile, for tissue engineering and regenerative medicine.

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confidence: 91%

Biofunctionalized pectin hydrogels as 3D cellular microenvironments

et al. 2015

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“…Various techniques are in use for this purpose including autoclaving [34][35][36] , ethylene oxide treatment 37 , microfiltration 38 , and antibiotic supplements 39 . Although no studies directly compare these methods with respect to their impacts on GG, equivalent investigations using other polysaccharides [40][41][42][43] and ECM materials 40,44,45 provide a good indication of the likely effects on GG. In general, these studies report that common sterilisation procedures are generally effective at removing or deactivating contaminants without loss of the biological activity.…”

Section: Purification and Sterilisationmentioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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“…Sterilization processes are intended to deal with contamination of food, surfaces, or equipment by potentially harmful life forms [48]. It involves the use of a physical or chemical procedure to destroy all microbial life [48], including highly resistant bacteria spores.…”

Section: Introductionmentioning

confidence: 99%

“…It involves the use of a physical or chemical procedure to destroy all microbial life [48], including highly resistant bacteria spores. To achieve it, there are various procedures, such as autoclaving, UV irradiation, microwaves and chemical techniques [44,49,50].…”

Section: Introductionmentioning

confidence: 99%