Steroid and anti-CD18 treatment have no effect on after-cataract formation following surgery in rabbits

Wallentin, Niklas; Lundberg, Claes

doi:10.1076/0271-3683(200005)20:5;1-1;ft384

Cited by 4 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eyes with uveitis at any point interval after the 2‐week evaluation received 1% prednisolone acetate, and/or diclofenac and/or 1% atropine solution, but frequency and duration were not evaluated. Although the use of anti‐inflammatory therapy 6 weeks postsurgically was not evaluated, in vitro and in vivo studies on pharmacologic attempts to reduce PCO have not revealed a decrease in PCO formation 40,50–53 …”

Section: Discussionmentioning

confidence: 99%

“…demonstrated that removal of greater than 95% residual LECs is necessary to prevent PCO formation 22,23 . In addition, pharmacologic attempts to inhibit mitosis and metaplasia of the LECs have not been successful or result in corneal endothelial toxicity 40,50–53 . Therefore, the main focus in eliminating LEC migration and visual axis opacification is designing a biocompatible IOL that reduces the acute foreign body reaction by decreasing macrophages and the severity of the inflammatory response 41,42 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Posterior capsular opacification in diabetic and nondiabetic canine patients following cataract surgery

Bras¹,

Colitz²,

Saville

et al. 2006

Veterinary Ophthalmology

View full text Add to dashboard Cite

Posterior capsular opacification (PCO) is the most common postoperative complication of contemporary cataract surgery. Limited information is available regarding PCO formation and factors that influence PCO development in the dog. Two hundred sixty-five eyes (144 from diabetic dogs and 121 from dogs with breed-related cataracts) were prospectively evaluated for PCO formation for up to 12 months postoperatively. The mean age of all dogs in the study was 7.77 years and diabetic dogs were significantly older than dogs with breed-related cataracts. There were 73 males (61 neutered, 12 intact) and 74 females (70 neutered, 4 intact) in the study. Statistical analysis was performed based on age, breed/size, gender, stage of cataract at the time of surgery, PCO score at each time point, breed-related vs. diabetic cataract, right eyes compared to left eyes, and presence/absence of uveitis. Age and gender did not significantly influence PCO formation. Small and medium-sized breeds developed significantly more PCO in comparison to the large/giant breeds at 2 weeks and 2-4 months postoperatively, but the differences were not significant at later time points. There was an overall significant increase in PCO formation in eyes with early immature cataracts when compared to other stages of cataract up to 4 months postoperatively but not at later time points. There were no statistical differences in PCO score at 6 months or at 1 year postoperatively in eyes with breed-related and diabetic cataracts. Right eyes did not differ from left eyes in PCO score. PCO score significantly increased over time in breed-related and diabetic groups and in the overall population. No difference was found in the degree of PCO formation in eyes with inflammation prior to or after surgery compared with those without inflammation. In summary, age, gender, presence of inflammation, and cause of cataract (breed-related vs. diabetes mellitus) do not influence the development of PCO in canine cataract dogs. Small and medium-sized breeds develop significant PCO earlier than larger breeds. It is important to note that all eyes from all dogs in this study developed PCO in a time dependent manner.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Posterior capsular opacification in diabetic and nondiabetic canine patients following cataract surgery

Bras¹,

Colitz²,

Saville

et al. 2006

Veterinary Ophthalmology

View full text Add to dashboard Cite

show abstract

“…prostaglandin, cytokines) [2,11,14]. Nevertheless, reduction of the early inflammatory response after cataract surgery was shown to be not relevant in PCO development [35,36]. Accordingly, bendazac was shown to possess modest anti-inflammatory activity, being unable to interfere with arachidonic acid metabolism [16,37] and to inhibit proinflammatory cytokines such as IL-1ß and TNF· release (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Bendazac Lysine Inhibition of Human Lens Epithelial Cell Adhesion to Polymethylmethacrylate Intraocular Lenses

Soldo¹,

Milanese²,

Pinza³

et al. 2004

Ophthalmic Res

View full text Add to dashboard Cite

The aim of the present work was to evaluate the effect of bendazac lysine on the human lens epithelial cell line HLE-B3 adhesion to polymethylmethacrylate (PMMA) intraocular lenses (IOLs). After adherence to IOLs, cells were incubated in the presence of the drug for 24 h. The number of cells contained in a 6-mm² area was then counted with an inverted phase microscope and adherent cells were distinguished from detached floating cells by focusing through the medium. Results obtained show that bendazac is able to induce a linear dose-dependent inhibition of HLE-B3 adhesiveness to PMMA IOLs. In particular, treatment with bendazac 33, 100 and 300 µM resulted in a 15, 32 and 54% inhibition, respectively. Statistical analysis shows that this effect is significant at 100 µM (p < 0.05) and 300 µM (p < 0.01). The analysis of the effects of bendazac on the viability and on the proliferative capacity of HLE-B3 cells did not show any drug-related toxicity up to the concentration of 400 µM. The present study demonstrates that bendazac lysine is able to inhibit adhesion of lens epithelial cells to PMMA IOLs and suggests the potential beneficial use of this drug in preventing secondary cataract development.

show abstract

“…Further, in protocol 2, the animals were treated with dexamethasone topically for 7 days after surgery. We have previously shown that treatment with steroids, prior to and at the time of surgery, as well as daily local treatment up to 1 month after surgery did not affect after-cataract wet weight [24][25][26][27]29].…”

Section: Discussionmentioning

confidence: 99%

Development of Posterior Capsule Opacification in the Rabbit

Wallentin

Lundgren²,

Holmén³

et al. 2002

Ophthalmic Res

View full text Add to dashboard Cite

Purpose: The aim of the present study was to characterize the development of after-cataract in the rabbit by measuring its wet weight, protein, DNA and glycosaminoglycan (GAG) contents and using Scheimpflug and slitlamp analysis. Further, aqueous humor (AqH) leukocytes, protein and lens epithelial cell proliferation activity were determined. Methods: AqH was collected and capsular bags including after-cataract were dissected free on days 0, 1, 7, 14, 28 and 56 after cataract surgery. The wet weights were determined and the contents of DNA, protein and GAG in the capsular bags including after-cataract were analyzed. AqH was analyzed for leukocytes, protein and proliferative activity. In another set of experiments, rabbit eyes were analyzed by the Scheimpflug technique and slitlamp examination on days 0, 1, 7, 14, 28 and 56 after cataract surgery. The wet weight of the capsular bag with the after-cataract was also determined. Results: An increase was found in the wet weight (480%) and the contents of protein (221%), DNA (945%) and GAG (336%) of the capsular bags including after-cataract during the experimental period. In the AqH, all 3 variables measured, leukocytes, protein and proliferative activity, reached their highest levels on day 1 after surgery. In the second set of experiments, the wet weight of the capsular bag including after-cataract increased by 391% during the 56-day experimental period. Posterior capsule opacification (PCO), as measured by Scheimpflug analysis, increased from 0.8 to 81.7% and the scores of Elschnig’s pearls as well as fibrosis, analyzed by slitlamp, increased from 0.0 to 2.8 and 3.0, respectively. Conclusions: This study shows that the same components that are reported to be important in human PCO are also components of PCO in the rabbit. Thus, the rabbit model seems to accurately reflect human PCO development, and because PCO develops much faster in rabbits that would make the rabbit model suitable for studies to elucidate human PCO development.

show abstract

Steroid and anti-CD18 treatment have no effect on after-cataract formation following surgery in rabbits

Abstract: This study indicates that reduction of the early inflammatory response after cataract surgery is not important in the development of after-cataract.

Cited by 4 publications

References 57 publications

Posterior capsular opacification in diabetic and nondiabetic canine patients following cataract surgery

Posterior capsular opacification in diabetic and nondiabetic canine patients following cataract surgery

Bendazac Lysine Inhibition of Human Lens Epithelial Cell Adhesion to Polymethylmethacrylate Intraocular Lenses

Development of Posterior Capsule Opacification in the Rabbit

Contact Info

Product

Resources

About