Steroid and xenobiotic receptor-mediated effects of bisphenol A on human osteoblasts

Miki, Yasuhiro; Hata, Shuko; Nagasaki, Shuji; Suzuki, Takashi; Itō, Kiyoshi; Kumamoto, Hiroyuki; Sasano, Hironobu

doi:10.1016/j.lfs.2016.05.013

Cited by 18 publications

(14 citation statements)

References 49 publications

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“…When considering other studies related to differentiation of MSCs, there is limited information about the toxic effect of BPA towards bone health [83]. It is known that BPA may affect adult bone metabolism and its disorders, including osteoporosis through the SXR signaling in both adult and fetal bones [84]. Several in vitro studies involving cultured scales of goldfish, bone-marrow derived macrophages, RAW 264.7 cells, and MC3 T3-E1 cells, as well as an in vivo study with ovariectomized rats have shown this relation [85][86][87][88][89][90].…”

Section: Osteogenic Differentiationmentioning

confidence: 99%

Bisphenol a and mesenchymal stem cells: Recent insights

et al. 2018

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Mesenchymal stem cells (MSCs) are found in all adult mesenchymal tissues. They play a role in the maintenance of tissue homeostasis and repair by allowing renewal of the cellular stock. MSCs can be isolated from both human and animal sources. These cells are important in regenerative medicine and cell therapy, thus adipose tissue is a rich and promising source of these cells. Adipose-derived stem cells (ASCs) are often effective and safe, and have been used in preclinical and clinical studies for both autologous and allogeneic transplantation. The potential use of stem cell-based therapies for the repair and regeneration of various tissues and organs provides an important alternative therapeutic solution for the treatment of many diseases. However, it is necessary to have control of the cell manipulation process prior to their use. Exposure of humans to the endocrine disruptor bisphenol A (BPA) has been associated with increased weight and obesity, but the mechanisms by which BPA increases adipose tissue in humans remains to be determined. BPA has been classified as a potent endocrine-disrupting chemical that interferes with adipogenesis. Currently, few studies have reported the effect of BPA on the integrity and capacity for differentiation of MSCs. Thus, this review aims to present, for the first time, a current survey and a discussion of the effects of BPA action on MSCs.

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Section: Osteogenic Differentiationmentioning

confidence: 99%

Bisphenol a and mesenchymal stem cells: Recent insights

et al. 2018

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“…The tissue specimens were incubated for 30 min at room temperature with a green dye (0.1% Fast Green FCF) that stains non-collagenous proteins and a red dye (0.1% Sirius Red F3B) that specifically stains collagen. The procedures of immunohistochemistry and histochemistry have been previously described in detail [ 4 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The human osteosarcoma cell line MG-63 was provided by the Cell Resource Center for Biomedical Research, Tohoku University (Sendai, Japan), and was maintained in RPMI-1640 medium (Sigma-Aldrich) (passage number: 30 to 40). The detailed culture conditions were reported in our previous studies [ 4 , 20 , 32 ]. The cells were pre-cultured for 48 h with 10% dextran-coated charcoal-stripped FBS (DCC-FBS) before examination.…”

Section: Methodsmentioning

confidence: 99%

“…Alkylphenols, such as nonylphenol and octylphenol, inhibit the formation of osteoclasts in co-cultures of murine osteoclast precursor cells with osteoclast-supporting cells [ 1 ]. EDCs also exert various effects on bone cells through nuclear receptors, such as the estrogen receptor (ER) [ 2 ], peroxisome proliferator-activated receptor γ (PPAR γ) [ 3 ], and steroid and xenobiotic receptor (SXR) [ 4 ]. Among these receptors, aryl hydrocarbon receptor (AhR) plays pivotal roles in mediating the action of EDCs.…”

Section: Introductionmentioning

confidence: 99%

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Roles of Aryl Hydrocarbon Receptor in Aromatase-Dependent Cell Proliferation in Human Osteoblasts

Miki

Hata

Ono

et al. 2017

IJMS

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Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and its expression is influenced by environmental compounds, such as 3-methylcholanthrene (3-MC) and β-naphthoflavone (β-NF). AhR and its downstream genes, such as CYP1A1, are considered to play a pivotal role in xenobiotic responses. AhR signaling has also been proposed to mediate osteogenesis in experimental animals, but its details have remained unclear. Therefore, in this study, we examined the possible roles of AhR in human bone. Immunohistochemical analysis revealed that AhR was detected in both osteoblasts and osteoclasts. We then screened AhR-target genes using a microarray analysis in human osteoblastic hFOB cells. Results of microarray and subsequent PCR analysis did reveal that estrogen metabolizing and synthesizing enzymes, such as CYP1B1 and aromatase, were increased by 3-MC in hFOB and osteosarcoma cell line, MG-63. The subsequent antibody cytokine analysis also demonstrated that interleukin-1β and -6 expression was increased by 3-MC and β-NF in hFOB cells and these interleukins were well known to induce aromatase. We then examined the cell proliferation rate of hFOB and MG-63 cells co-treated with 3-MC and testosterone as an aromatase substrate. The status of cell proliferation in both hFOB and MG-63 cells was stimulated by 3-MC and testosterone treatment, which was also inhibited by an estrogen blocker, aromatase inhibitor, or AhR antagonist. These findings indicated that AhR could regulate estrogen synthesis and metabolism in bone tissues through cytokine/aromatase signaling.

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“…For example, in osteosarcoma cells, PXR activation is associated with chemo-resistance (Mensah-Osman et al, 2007). In this context, human PXR transfected normal osteoblast cells (hFOB) show increased proliferation in culture when exposed to Bisphenol A, a human PXR ligand (Miki et al, 2016). In Barrett’s esophagus patients, PXR expression is higher in high-grade versus low-grade dysplasia, suggesting that PXR may be involved in tumor progression in Barrett’s esophagus (van de Winkel et al, 2011b).…”

Section: Differential Role Of Pxr In Cancermentioning

confidence: 99%

Pregnane X Receptor and Cancer: Context-Specificity is Key

Pondugula

Pávek

Mani

2016

Nuclear Receptor Research

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Pregnane X receptor (PXR) is an adopted orphan nuclear receptor that is activated by a wide-range of endobiotics and xenobiotics, including chemotherapy drugs. PXR plays a major role in the metabolism and clearance of xenobiotics and endobiotics in liver and intestine via induction of drug-metabolizing enzymes and drug-transporting proteins. However, PXR is expressed in several cancer tissues and the accumulating evidence strongly points to the differential role of PXR in cancer growth and progression as well as in chemotherapy outcome. In cancer cells, besides regulating the gene expression of enzymes and proteins involved in drug metabolism and transport, PXR also regulates other genes involved in proliferation, metastasis, apoptosis, anti-apoptosis, inflammation, and oxidative stress. In this review, we focus on the differential role of PXR in a variety of cancers, including prostate, breast, ovarian, endometrial, and colon. We also discuss the future directions to further understand the differential role of PXR in cancer, and conclude with the need to identify novel selective PXR modulators to target PXR in PXR-expressing cancers.

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Steroid and xenobiotic receptor-mediated effects of bisphenol A on human osteoblasts

Cited by 18 publications

References 49 publications

Bisphenol a and mesenchymal stem cells: Recent insights

Bisphenol a and mesenchymal stem cells: Recent insights

Roles of Aryl Hydrocarbon Receptor in Aromatase-Dependent Cell Proliferation in Human Osteoblasts

Pregnane X Receptor and Cancer: Context-Specificity is Key

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