Steroid formation by the avian ovary in vitro (Gallus domesticus)

Boucek, Robert J.; Savard, Kenneth

doi:10.1016/0016-6480(70)90090-0

Cited by 35 publications

(18 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of acid phosphatase can be attributed to the transport of substances through the follicular epithelium. In contrast to previous observations on fowl (Chiefli and Botte 1965;Boucek and Savard 1970), no cytochemical feature related to steroidogenesis has been observed in the follicular epithelium of maturing follicle.…”

Section: Discussioncontrasting

confidence: 54%

“…Previous electron microscopic studies have demonstrated that the follicle cells in the bird ovary are specialised for protein synthesis (Bellairs 1965 ;Peel and Bellairs 1972) whereas the histochemical studies have suggested that they are involved in steroid synthesis (Chiefli and Botte I965; Boucek and Savard, 1970). Most of the previous work is confined to the ovarian changes in fowl but little is known about the sequential morphological and histochemical alterations in the follicular epithelium during oocyte growth in wild bird species.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and histochemical changes in the follicular epithelium during follicular growth in the crowCorvus splendens

Chalana

Guraya

1980

Proc. Indian Acad. Sci. (Anim. Sci.)

View full text Add to dashboard Cite

MS received 16 May 1979Al~atraet. Morphological and histochemical changes in the follicular epithelium have been studied during folliculogenesis in the crow (Corvus splendens) ovary. The follicular epithelium is single-layered in primordial and early previtellogenic, pseudostratified in late-previtellogenic, multilayered in early vitellogenic and again becomes single-layered in late-vitellogenic oocytes. Two types of follicle cells have been distinguished on the basis of their structure and cytochemical properties. Some of the follicle ceils separate from the follicular epithelium and invade the ooplasm where they seem to be reabsorbed by the latter. The amount oflipids and proteins increases in the follicular epithelium as the oocyte grows. Simultaneously the nature of lipids and proteins changes, as revealed by various histoehemical reactions. The follicular epithelium, at all stages of follicular growth, shows moderate reactions for acid phosphatase and NAD-diaphorase. The significance of the structural and histochemical changes in the follicular epithelium during oogenesis have been discussed.

show abstract

Section: Discussioncontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Morphological and histochemical changes in the follicular epithelium during follicular growth in the crowCorvus splendens

Chalana

Guraya

1980

Proc. Indian Acad. Sci. (Anim. Sci.)

View full text Add to dashboard Cite

show abstract

“…In the present investi¬ gation, no attempt was made to determine whether the follicles or the stroma were responsible for the high levels of oestradiol found. Boucek & Savard (1970) demonstrated the presence of the enzymes A5-3/?-hydroxysteroid oxidoreductase and 17/Miydroxysteroid oxido-reductase in ovarian stromal cells.…”

Section: Oestradiol In Ovarian Tissuementioning

confidence: 92%

“…Certainly, the major follicles contain enzymes necessary for oestrogen biosynthesis (Chieffi & Botte, 1965;Boucek & Savard, 1970) and further investigations are essential to determine whether a large increase in oestradiol output by the follicles occurs 4 to 8 hr before ovulation.…”

Section: Oestradiol In Ovarian Tissuementioning

confidence: 99%

A Preliminary Assessment of the Source of Oestrogen Within the Ovary of the Domestic Fowl, Gallus Domesticus

Senior¹,

Furr²

1975

Reproduction

View full text Add to dashboard Cite

Summary. Oestradiol was measured by radioimmunoassay in blood draining individual preovulatory follicles and in different ovarian tissues of the hen. The concentration of oestradiol in blood from follicles 20 to 50 hr before ovulation ranged from 66 to 264 pg/ml and was less than that in peripheral blood collected concurrently, suggesting a net uptake rather than secretion of oestradiol by the follicle at these times.In one bird approximately 6 hr before ovulation, the highest content of oestradiol in tissue (35 ng) was in the small (<5 mm) follicles and ovarian stroma; this represented 87\m=.\5% of the total ovarian content. Only 0\m=.\86 to 1\m=.\02ng oestradiol (2\m=.\0 to 2\m=.\5%) was found in the large preovulatory follicles and 2\m=.\22 ng (5\m=.\5%) in the postovulatory follicles. High concentrations of oestradiol (2\m=.\4ng/g) were also found in the liver.These results suggest that the small follicles and/or ovarian stroma are the main site ofoestradiol production in the fowl; they do not exclude the possibility that oestradiol is secreted at a high rate by the mature follicle for a short period immediately before ovulation, thus influencing the release of LH.

show abstract

“…These characteristics confer on the cells a steroidogenic function. In adult hens, the presence of D 5 -3b HSD in the ovary has been detected in the granulosa and thecal interstitial cells predominantly associated with microsomes (Boucek and Savard, 1970;Armstrong and Wells, 1976).…”

Section: Figure 2 Number Of Somatic Cells Without Lipid Droplets In mentioning

confidence: 98%

Effect of luteinising-hormonein vivoon ovarian cell subpopulations of newly-hatched chicks

González-Morán

Calderon²

2000

British Poultry Science

View full text Add to dashboard Cite

1. We analysed the number and size of different ovarian cell subpopulations of newly-hatched chicks by ovarian cell suspension count and morphometric/stereological methods as well as delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta HSD) activity in these cells treated in vivo with LH during embryonic development. 2. Fertile White Leghorn eggs received 1 microg LH applied to the chorioallantoic membrane on days 13, 15, and 17 of incubation. All animals were killed within 24 h after hatching, the left ovary was dissected and processed. 3. The results indicate that the number of germ, pregranulosa, interstitial and undifferentiated cells was not affected by LH treatment. However, we observed an increase in the size of individual interstitial cells of the ovarian medulla. In these cells, delta5-3beta HSD activity was increased in response to LH. 4. These findings suggest that LH does not exert a proliferative effect on the cells of the prefollicular ovary of the chick and that interstitial cells can be target cells for LH, increasing their steroidogenic activity due to LH treatment.

show abstract

Steroid formation by the avian ovary in vitro (Gallus domesticus)

Cited by 35 publications

References 9 publications

Morphological and histochemical changes in the follicular epithelium during follicular growth in the crowCorvus splendens

Morphological and histochemical changes in the follicular epithelium during follicular growth in the crowCorvus splendens

A Preliminary Assessment of the Source of Oestrogen Within the Ovary of the Domestic Fowl, Gallus Domesticus

Effect of luteinising-hormonein vivoon ovarian cell subpopulations of newly-hatched chicks

Contact Info

Product

Resources

About