Steroidal Constituents of Costus speciosus (Koen) Sm. Callus cultures

Abstract

Undifferentiated callus cultures of Costus speciosus yielded diosgenin, tigogenin, lanosterol and stigmasterol.-Very recently,

Production of Diosgenin in Somatic Callus Tissues ofDioscorea floribunda

“…Importance of diosgenin, a steroidal sapogenin, has prompted extensive investigations of Dioscorea species. Diosgenin has been reported in tissue cultures of many plants, viz., Costus speciosus [1], D. cornposita [2,3], D. deltoidea [4][5][6], D. floribunda [7, 81, Solanurn spp. [9][10][11][12], Trigonella foenurn-graecum [13], Daucus carota [141, Momordica charantia [15], etc.…”

Steroidal Constituents ofCostus afar

“…Varied amounts of diosgenin have been reported from different samples of C. speciosus; the highest yield being 2.12 %. Callus cultures of C. speciosus also produced diosgenin in good yield together with lanosterol, tigogenin and stigmasterol [5]. The present study is on the steroidal content of C. afar and estimation of the diosgenin yield of its rhizomes and the ae- An aliquot (Z) of this solution was taken, such that it produced an absorbance equivalent to between 20 and 100 ug of diosgenin (the most accurate region of the standard curve).…”

Distribution and Variation of Diosgenin in Different Parts of Costus speciosus

“…They also reported the presence of free diosgenin and tigogenin in its rhizome. Further, Rathore and Khanna (2) reported that rhizomes of C. speciosus also contained lanosterol and stigmasterol besides the diosgenin found in the stem (3). Free diosgenin, however, has not been reported in any other part of the plant except the rhizome.…”