Steroidobacter soli sp. nov., isolated from farmland soil

Huang, Junwei; Hu, Shunli; Cheng, Xiaokun; Chen, Dian; Kong, Xianhui; Jiang, Jiandong

doi:10.1099/ijsem.0.003639

Cited by 18 publications

(5 citation statements)

References 27 publications

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“…1 2 3 4 5 6 7 8 Colony color pale yellow pale yellow pale yellow pale yellow yellow-brown yellow-orange light yellow, then turns to yellow light brown Motility non-motile non-motile non-motile motile motile by a single polar flagellum motile by 1 (or 2) polar flagellum motile by a single polar flagellum motile by a single polar flagellum Cell morphology Rod rod straight to slightly curved rod straight to slightly curved rod slightly curved rod rod rod rod Cell size width by length (μm) 0.4-0.6 by 1.5-2.5 0.4-0.7 by 1.3-2.0 0.4-0.6 by 1.0-2.1 0.3-0.5 by 1.0-3.1 0.3-0.5 by 0.6-1.6 0.4-0.6 by 2.0-5.0, occasionally longer (10 μm) 0.6-0.8 by 1.5-1.8 0.3-0.5 by 1.0-3. 6.0-12.0 (7.0-7.5) 6.0-12.0 (7.0) 4.5-9.0 (6.0-8.0) 6.0-9.0 (7.0) 6.1-7.8 (7.0) ND (ND) 6.0-8.0 (7.0-7.5) 6.5-9.0 (7.5 or 7.0) Growth range at different NaCl concentrations (optimum) (%) 0-2.0 (1.0) 0-3.0 (1.0) 0-2.0 (ND) 0-1.0 (0.5) ND (0.1) ND (ND) 0-3.0 (ND) 0-0.5 (0.1) Taxa: 1 strain SA29-B T ; 2 strain YU21-B; 3, Steroidobacter agariperforans KA5-B T (Suyama et al, 1993); 4, Steroidobacter soli JW-3 T (Huang et al, 2019); 5, Steroidobacter denitrificans FS T (Fahrbach et al, 2008); 6, "Steroidobacter cummioxidans" 35Y (Sharma et al, 2018); 7, "Steroidobacter flavus" CPCC 100154 (Gong et al, 2016); 8, Povalibacter uvarum Zumi 37 T (Nogi et al, 2014). Sum3, summed feature 3, is composed of C16:1ω7c and C16:1ω6c.…”

Section: / 10 Article Me20136mentioning

confidence: 99%

Characterization and Distribution of Agar-degrading <i>Steroidobacter agaridevorans</i> sp. nov., Isolated from Rhizosphere Soils

et al. 2021

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The environment of plant rhizosphere soil differs from that of non-rhizosphere soil due to the secretion of mucilage polysaccharides from the roots. This environment is regarded as one of the preferential habitats for agar-degrading bacteria. In a previous study, agar-degrading Steroidobacter agariperforans KA5-B T was isolated from agar-enriched agricultural soil using diffusible metabolites from Rhizobiales bacteria. Based on the hypothesis that similar characteristic bacteria still exist in the rhizosphere, isolation was performed using rhizosphere soils. Agar-degrading SA29-B T and YU21-B were isolated from onion and soybean rhizosphere soils. The 16S rRNA genes of these strains showed ≥98.7% identities with the most closely related strain KA5-B T. However, differences were noted in polysaccharide utilization, and average nucleotide identities were <95-96% against strain KA5-B T , indicating that they are different species from S. agariperforans KA5-B T. To investigate the distribution of bacterial sequences affiliated with novel strains, a primer set was designed and a metaanalysis of the 16S rRNA gene was performed. Sequences were widely distributed in rhizospheres throughout Japan, but varied in plant-and region-dependent manners. Regarding phenotypic characterization, distinguishable features were observed in growth temperatures, pH, and dominant fatty acids. SA29-B T and YU21-B grew at 15-40°C and pH 6.0-12 and contained C 16:0 as the dominant cell fatty acid, whereas KA5-B T showed no growth at 40°C and pH 12 and contained a moderate amount of C 16:0. Based on these characteristics, SA29-B T (JCM 333368 T =KCTC 72223 T) and YU21-B (JCM 333367=KCTC 72222) represent novel species in the genus Steroidobacter, for which the name Steroidobacter agaridevorans sp. nov. is proposed.

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Section: / 10 Article Me20136mentioning

confidence: 99%

Characterization and Distribution of Agar-degrading <i>Steroidobacter agaridevorans</i> sp. nov., Isolated from Rhizosphere Soils

et al. 2021

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“…After autoclaving, the pH was rechecked and recorded as the final pH of the growth medium. Tolerance to NaCl was tested at serially increased concentrations (0–3 % at 0.5 % intervals and 3–7 % at 1 % intervals, w/v) according to the formula for R2A broth medium [3].…”

Section: Morphology and Physiologymentioning

confidence: 99%

“…agaridevorans' SA29-B (data adapted from [6]); 4, S. agariperforans KA5-B T (data adapted from [2]); 5, 'S. flavus' CPCC 100154 (data adapted from [4]); 6, S. soil JW-3 T (data adapted from [3]); 7, S. denitrifcans FS T (data adapted from [1]). Symbols: +, positive; −, negative; w, weakly positive; nd, not determined or no data available.…”

Section: Table 1 Differential Characteristics Between Strain S1-65 T and Closely Related Species In The Genus Steroidobactermentioning

confidence: 99%

“…Strains: 1, S1-65 T (data from this study); 2, 'S. agaridevorans' SA29-B (data adapted from [6]); 3, S. agariperforans KA5-B T (data adapted from [2]); 4, S. soil JW-3 T (data adapted from [3]); 5, S. denitrifcans FS T (data adapted from [1]); 6, 'S. flavus' CPCC 100154 (data adapted from [4]).…”

Section: Table 2 Cellular Fatty Acid Composition Of Strain S1-65 T and Closely Related Species In The Genus Steroidobactermentioning

confidence: 99%

“…The genus Steroidobacter , belonging to the family Sinobacteraceae of the class Gammaproteobacteria was first proposed by Fahrbach et al [1], in which Steroidobacter denitrificans FS T was initially proposed as the type species of the genus. Two novel species, Steroidobacter agariperforans KA5-B T [2] and Steroidobacter soli JW-3 T [3], were subsequently approved. ‘ Steroidobacter flavus ’ CPCC 100154 [4] and ‘ Steroidobacter cummioxidans ’ 35Y [5] have also been proposed, but not validly published.…”

Section: Introductionmentioning

confidence: 99%

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Steroidobacter gossypii sp. nov., isolated from cotton field soil

Huang

Chen

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative bacterium, designated S1-65T, was isolated from soil samples collected from a cotton field located in the Xinjiang region of PR China. Results of 16S rRNA gene sequence analysis revealed that strain S1-65T was affiliated to the genus Steroidobacter with its closest phylogenetic relatives being ‘Steroidobacter cummioxidans’ 35Y (98.4 %), ‘ Steroidobacter agaridevorans ’ SA29-B (98.3 %) and Steroidobacter agariperforans KA5-BT (98.3 %). 16S rRNA-directed phylogenetic analysis showed that strain S1-65T formed a unique phylogenetic subclade next to ‘ S. agaridevorans ’ SA29-B and S. agariperforans KA5-BT, suggesting that strain S1-65T should be identified as a member of the genus Steroidobacter . Further, substantial differences between the genotypic properties of strain S1-65T and the members of the genus Steroidobacter , including average nucleotide identity and digital DNA–DNA hybridization, resolved the taxonomic position of strain S1-65T and suggested its positioning as representing a novel species of the genus Steroidobacter . The DNA G+C content of strain S1-65T was 62.5 mol%, based on its draft genome sequence. The predominant respiratory quinone was ubiquinone-8. The main fatty acids were identified as summed feature 3 (C16:1ω6c/C16:1ω7c), C16 : 0 and iso-C15 : 0. In addition, its polar lipid profile was composed of aminophospholipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Here, we propose a novel species of the genus Steroidobacter : Steroidobacter gossypii sp. nov. with the type strain S1-65T (=JCM 34287T=CGMCC 1.18736T).

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