Steroidogenesis in Patients with Various Adrenal Cortex Diseases as Studied by Reversed-Phase High-Performance Liquid Chromatography

Kartsova, A. A.; Великанова, Л И; Pavlova, E. G.; Бессонова, Е. А.

doi:10.1023/b:janc.0000043915.76485.e0

Cited by 5 publications

(8 citation statements)

References 7 publications

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“…The purity of the solvent is essential because during the pre-concentration process the concentration of any impurities increases. This type of extraction during the analysis of steroids has been employed by many researchers [14,15,16,17,18]. The main conclusions of these studies were that the extracts obtained were often found to be “too dirty” for further analysis, acidic or basic wash steps had to be introduced to remove any interferences, large volumes of organic solvents were needed and analyte recoveries were frequently very low.…”

Section: Resultsmentioning

confidence: 99%

“…Hypercortisolism is frequently associated not only with an excess of glucocorticoid effects, but also with an increase in mineralocorticoid activity. The cortisol/cortisone ratio is particularly elevated in patients with ectopic adrenocorticotropic hormone (ACTH) syndrome [17].…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, they may be present in high concentrations and interfere with the signals from the analytes of interest. As can be seen from the literature, researchers studying the determination of steroid hormones have employed different extraction methods: liquid-liquid extraction (LLE) [14,15,16,17,18], solid phase extraction (SPE) [19,20,21,22,23] or solid phase microextraction (SPME) [4].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of a Pre-MEKC Separation SPE Procedure for Steroid Molecules in Human Urine Samples

et al. 2013

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Many steroid hormones can be considered as potential biomarkers and their determination in body fluids can create opportunities for the rapid diagnosis of many diseases and disorders of the human body. Most existing methods for the determination of steroids are usually time-and labor-consuming and quite costly. Therefore, the aim of analytical laboratories is to develop a new, relatively low-cost and rapid implementation methodology for their determination in biological samples. Due to the fact that there is little literature data on concentrations of steroid hormones in urine samples, we have made attempts at the electrophoretic determination of these compounds. For this purpose, an extraction procedure for the optimized separation and simultaneous determination of seven steroid hormones in urine samples has been investigated. The isolation of analytes from biological samples was performed by liquid-liquid extraction (LLE) with dichloromethane and compared to solid phase extraction (SPE) with C 18 and hydrophilic-lipophilic balance (HLB) columns. To separate all the analytes a micellar electrokinetic capillary chromatography (MECK) technique was employed. For full separation of all the analytes a running buffer (pH 9.2), composed of 10 mM sodium tetraborate decahydrate (borax), 50 mM sodium dodecyl sulfate (SDS), and 10% methanol was selected. The methodology developed in this work for the determination of steroid hormones meets all the requirements of analytical methods. The applicability of the method has been confirmed for the analysis of urine samples collected from volunteers-both men and women (students, amateur bodybuilders, using and not applying steroid doping). The data obtained during this work can be successfully used for further research on the determination of steroid hormones in urine samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of a Pre-MEKC Separation SPE Procedure for Steroid Molecules in Human Urine Samples

et al. 2013

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“…Várias técnicas para a determinação de corticosteróides em fluidos biológicos necessitam do pré-tratamento da amostra [6,9,19,26,34,35,36,37,38,39,40,41] seja através de métodos de extração em fase sólida ou extração por solventes (diclorometano, acetato de etila, metanol, entre outros). Vale ressaltar que o pré-tratamento da amostra pode induzir a alguns problemas, como: o tempo dispensado no processo, manuseio laborioso, erros de manipulação, contaminação por outros agentes, alto custo, decomposição de alguns compostos, entre outros.…”

Section: Corticosterona Acetato De Hidrocortisona Acetato De Dexametaunclassified

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

2010

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The present work had as aim the development of simple an analytical method, fast efficient and the determination of corticosteroids (cortisone, corticosterone, acetate of hidrocortisone and acetate of dexametasone) in sanguine samples of rats using an isocratic system of liquid chromatography of high efficiency (CLAE) with ultraviolet detention. The methodinvolved direct injection of sanguine sample in a chromatographic column with internal surface of phase reverse (ISRP-C18), using the composed mobile phase for drain plug phosphate pH 4,0: acetonitrile (65:35 v/v). The detention the analytes was obtained using an ultraviolet detector of changeable wave length (Varian Model 2550) adjusted in 240 nm and a 4400 integratorSP Chromaject (Varian Associates, Inc., Sunnyvale, CA, USA). The extraction of the analyte resulted in recovery values between 90% and 108%, and variation coefficient enter 1,1% and 2.5%. The detention and quantification limits of the method were 0,02 and 0,04 μg mL-1, respectively. In conclusion, the analytical method possible the direct injection (on-line) of the sample without previous treatment also presenting some advantages, such as: fast, exactness, precision and specific.

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“…Várias técnicas para a determinação de corticosteróides em fluidos biológicos necessitam do prétratamento da amostra [6,9,19,26,34,35,36,37,38,39,40,41] seja através de métodos de extração em fase sólida ou extração por solventes (diclorometano, acetato de etila, metanol, entre outros). Vale ressaltar que o pré-tratamento da amostra pode induzir a alguns problemas, como: o tempo dispensado no processo, manuseio laborioso, erros de manipulação, contaminação por outros agentes, alto custo, decomposição de alguns compostos, entre outros.…”

Section: Cortisonaunclassified

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

2010

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resumo: O presente trabalho teve como objetivo o desenvolvimento de um método analítico simples, rápido e eficiente para a determinação de corticosteróides (cortisona, corticosterona, acetato de hidrocortisona e acetato de dexametasona) em amostras sangüíneas de ratos empregando um sistema isocrático de cromatografia líquida de alta eficiência (CLAE) com detecção ultravioleta. O método envolveu a injeção direta da amostra sangüínea em uma coluna cromatográfica com superfície interna de fase reversa (ISRP-C 18 ), empregando a fase móvel composta por tampão fosfato pH 4,0: acetonitrila (65:35 v/v). A detecção dos analitos foi obtida, através de um detector de ultravioleta de comprimento de onda variável (Varian Modelo 2550) ajustado em 240 nm e um integrador SP 4400 Chromaject (Varian Associates, Inc, Sunnyvale, CA, USA). A extração do analito resultou em valores de recuperação entre 90% e 108%, e coeficiente de variação entre 1,1% a 2,5%. Os limites de detecção e quantificação do método foram de 0,02 e 0,04 µg mL -1 , respectivamente. Assim, o método analítico proposto possibilitou a injeção direta (on-line) da amostra sem tratamento prévio apresentando também várias vantagens, tais como: rapidez, exatidão, precisão e especificidade. palavras-chave: corticosteróides, amostra biológica, coluna ISRP-C 18 , CLAE. introdução CorticosteróidesOs corticosteróides também chamados de adrenocorticosteróides são hormônios de natureza esteroídica produzidos na porção cortical das glândulas adrenais, que estão localizados exata-mente no pólo superior de ambos os rins [1]. Estes hormônios são sintetizados a partir do colesterol e constituem três famílias: os glicocorticóides, os mineralocorticóides e os andrógenos adrenais. Os glicocorticóides atuam primariamente no metabolismo das proteínas, carboidratos e lipídios, e os principais hormônios endógenos são o cortisol e a corticosterona, como exemplos de sintéticos: cortisona, prednisona, metilprednisona, dexametasona, entre outros [2]. Os mineralocorticóides atuam

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Steroidogenesis in Patients with Various Adrenal Cortex Diseases as Studied by Reversed-Phase High-Performance Liquid Chromatography

Cited by 5 publications

References 7 publications

Optimization of a Pre-MEKC Separation SPE Procedure for Steroid Molecules in Human Urine Samples

Optimization of a Pre-MEKC Separation SPE Procedure for Steroid Molecules in Human Urine Samples

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

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