Steroidogenic effect of Erxian decoction for relieving menopause via the p-Akt/PKB pathway in vitro and in vivo

Wang, Shi Wei; Cheung, Ho Pan; Tong, Yao; Lu, Jia; Ng, Tzi Bun; Zhang, Yan Bo; Zhang, Zhang-Jin; Lee, Ckf; Lam, Jenny K.W.; Sze, Stephen Cho Wing

doi:10.1016/j.jep.2016.11.018

Cited by 19 publications

(12 citation statements)

References 32 publications

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“…8 Traditional Chinese medicine (TCM) has promising effects on treating gynecological endocrinology dysfunctions. [9][10][11] Gengnianchun (GNC) is a previously widely reported formula in the treatment of menopausal syndrome 12 and menopause-related diseases such as Alzheimer's. 13 According to TCM theories, DOR shares a similar etiology with menopause, which is deficient in the kidney.…”

Section: Introductionmentioning

confidence: 99%

<p>Therapeutic Effects of Modified Gengnianchun Formula on Stress-Induced Diminished Ovarian Reserve Based on Experimental Approaches and Network Pharmacology</p>

Gao¹,

Zhang²,

Xu³

et al. 2020

DDDT

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Section: Introductionmentioning

confidence: 99%

<p>Therapeutic Effects of Modified Gengnianchun Formula on Stress-Induced Diminished Ovarian Reserve Based on Experimental Approaches and Network Pharmacology</p>

Gao¹,

Zhang²,

Xu³

et al. 2020

DDDT

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“…Unlimited replicative potential of tumor cells is a hallmark of aggressive cancers [20]. Elevated levels of p-Akt/PKB have been shown to positively correlate with migrating breast cancer cells [21].…”

Section: Discussionmentioning

confidence: 99%

Deltex1 promotes cell proliferation and invasion and associated with clinical characteristics of breast cancer

wang

Liu

et al. 2020

Preprint

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Background Deltex1 (DTX1) was found to play its role in the development of several kinds of cancer. The association between DTX1 and breast cancer was unclear. We aimed to investigate the association between DTX1 expression and clinical characteristics of breast cancer, further explore the possible mechanisms of DTX1 on the development of breast cancer. Methods We determined level of DTX1 expression by qRT-PCR, analyzed association between DTX1 and clinical characteristics of breast cancer by student’s test and ANOVA test. The possible mechanisms of DTX1 on the development of breast cancer were explored with breast cancer cell lines in vitro. Results Levels of DTX1 in breast cancer tissues were lower compared to FCD tissues and peri-neoplastic breast tissues (P<0.01). Lower expression of DTX1 was associated with advanced tumor grade (grade III, P=0.002), advanced clinical TNM stage (III-IV stage, P=0.017), positive lymph node metastasis (P=0.034) and high Ki-67 index (P=0.031). Multivariate Cox regression analysis revealed that DTX1 expression was recognized as an independent prognostic factor for MFS in breast cancer. Elevated levels of Notch1, Jagged1 and HES1 in NOTCH pathway and p-Akt/PKB in PI3K/AKT pathway were found in breast cancer cells with lower DTX1 expression. GSI treatment could inhibit breast cancer cell proliferation and migration through NOTCH/DTX1/PI3K/AKT pathways. Conclusion Lower DTX1 in breast tissues was associated with advanced condition, prognosis and metastasis of breast cancer. Lower DTX1 might promote breast cancer cell proliferation and migration via NOTCH/DTX1/PI3K/AKT pathways. DTX1 might be useful as a marker to select new therapy for breast cancer.

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“…However, estrogen interacts with ERα and ERβ downstream pathways in dissimilar ways (Nilsson et al, 2001 ). ERβ regulates the FSH-aromatase pathway and enhances ovarian steroidogenesis (Deroo et al, 2009 ; Wang et al, 2017 ). It has been shown that selective activation of ERβ transcriptional pathways may not promote breast cancer (Paruthiyil et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Steroidogenic Components Derived From Gardenia jasminoides Ellis Potentially Useful for Treating Postmenopausal Syndrome

Wang

Rong

et al. 2018

Front. Pharmacol.

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Estrogen-stimulating principles have been demonstrated to relieve postmenopausal syndrome effectively. Gardenia jasminoides Ellis (GJE) is an herbal medicine possessing multiple pharmacological effects on human health with low toxicity. However, the therapeutic effects of GJE on the management of postmenopausal syndrome and its mechanism of action have not been fully elucidated. In this study, network pharmacology-based approaches were employed to examine steroidogenesis under the influence of GJE. In addition, the possibility of toxicity of GJE was ruled out and four probable active compounds were predicted. In parallel, a chromatographic fraction of GJE with estrogen-stimulating effect was identified and nine major compounds were isolated from this active fraction. Among the nine compounds, four of them were identified by network pharmacology, validating the use of network pharmacology to predict active compounds. Then the phenotypic approaches were utilized to verify that rutin, chlorogenic acid (CGA) and geniposidic acid (GA) exerted an estrogen-stimulating effect on ovarian granulosa cells. Furthermore, the results of target-based approaches indicated that rutin, CGA, and GA could up-regulate the FSHR-aromatase pathway in ovarian granulosa cells. The stimulation of estrogen production by rat ovarian granulosa cells under the influence of the three compounds underwent a decline when the follicle-stimulating hormone receptor (FSHR) was blocked by antibodies against the receptor, indicating the involvement of FSHR in the estradiol-stimulating activity of the three compounds. The effects of the three compounds on estrogen biosynthesis- related gene expression level were further confirmed by Western blot assay. Importantly, the MTT results showed that exposure of breast cancer cells to the three compounds resulted in reduction of cell viability, demonstrating the cytotoxicity of the three compounds. Collectively, rutin, chlorogenic acid and geniposidic acid may contribute to the therapeutic potential of GJE for the treatment of postmenopausal syndrome.

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Steroidogenic effect of Erxian decoction for relieving menopause via the p-Akt/PKB pathway in vitro and in vivo

Cited by 19 publications

References 32 publications

<p>Therapeutic Effects of Modified Gengnianchun Formula on Stress-Induced Diminished Ovarian Reserve Based on Experimental Approaches and Network Pharmacology</p>

<p>Therapeutic Effects of Modified Gengnianchun Formula on Stress-Induced Diminished Ovarian Reserve Based on Experimental Approaches and Network Pharmacology</p>

Deltex1 promotes cell proliferation and invasion and associated with clinical characteristics of breast cancer

Identification of Steroidogenic Components Derived From Gardenia jasminoides Ellis Potentially Useful for Treating Postmenopausal Syndrome

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