Steroids

Development of a sensitive and selective method for the quantitative analysis of cortisol, cortisone, prednisolone and prednisone in human plasma

“…1), analytical methods must be selective enough to distinguish between the synthetic and the endogenous corticosteroids. Previously reported analytical procedures for the determination of these compounds, either simultaneously or individually in plasma or serum, have employed a variety of techniques such as radioimmunological or competitive protein binding principles [6,7], micellar electrokinetic capillary chromatography (MECC) [8], high performance liquid chromatography (HPLC) with UV detection [9][10][11][12], fluorescence detection [13] or diode array detection coupled with second-order calibration [14], gas chromatography-stable isotope dilution mass spectrometry [15], gas chromatography-mass spectrometry (GC-MS) after purification by immunoaffinity chromatography [16] and liquid chromatography-mass spectrometry or tandem mass spectrometry [17][18][19][20].…”

Prednisolone Binding to Plasma Proteins in Domestic Species

“…3 In order to discuss interspecies prednisolone disposition, more information about plasma protein binding is required. The aim of the present experiments was to characterize prednisolone plasma protein binding in four domestic species, namely, horse, cow, sheep, and dog.…”

Oral Hydrocortisone Pharmacokinetics: A Comparison of Fluorescence and Ultraviolet High-Pressure Liquid Chromatographic Assays for Hydrocortisone in Plasma

“…Most of these employ UV detection of underivatized hydrocortisone (1)(2)(3)(4)(5)(6)(7), while others are based on detection of a fluorescent hydrocortisone derivative (8,9).…”