Stigmatic imaging of secondary ions in MeV-SIMS spectrometry by linear Time-of-Flight mass spectrometer and the TimePix detector

Jenčič, Boštjan; Šepec, Luka; Vavpetič, Primož; Kelemen, Mitja; Rupnik, Zdravko; Vencelj, M.; Vogel‐Mikuš, Katarina; Ogrinc, Nina; Ellis, Shane R.; Heeren, Ron M. A.; Pelicon, Primož

doi:10.1016/j.nimb.2019.05.040

Cited by 7 publications

(5 citation statements)

References 34 publications

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“…Subsequently, the influence of the custom-built TOF analyzer and the associated ion optics on the ion trajectories was investigated by the visualization of the spatial distribution of the ion cloud with axial and orthogonal imaging detectors. The MCP-TPX quad − ,,− and MCP-P47-TPX3CAM ,,− assemblies coupled with the Orbitrap/TOF instrument are deployed for this purpose (Configuration 1b, Figures S1–S2). Singly charged CsI ions of mass 5589 Da were selected using the quadrupole mass filter.…”

Section: Resultsmentioning

confidence: 99%

An Orbitrap/Time-of-Flight Mass Spectrometer for Photofragment Ion Imaging and High-Resolution Mass Analysis of Native Macromolecular Assemblies

Mathew

Giskes

Lekkas

et al. 2023

J. Am. Soc. Mass Spectrom.

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We discuss the design, development, and evaluation of an Orbitrap/time-of-flight (TOF) mass spectrometry (MS)-based instrument with integrated UV photodissociation (UVPD) and time/mass-to-charge ratio (m/z)-resolved imaging for the comprehensive study of the higher-order molecular structure of macromolecular assemblies (MMAs). A bespoke TOF analyzer has been coupled to the higher-energy collisional dissociation cell of an ultrahigh mass range hybrid quadrupole-Orbitrap MS. A 193 nm excimer laser was employed to photofragment MMA ions. A combination of microchannel plates (MCPs)-Timepix (TPX) quad and MCPs-phosphor screen-TPX3CAM assemblies have been used as axial and orthogonal imaging detectors, respectively. The instrument can operate in four different modes, where the UVPD-generated fragment ions from the native MMA ions can be measured with high-mass resolution or imaged in a mass-resolved manner to reveal the relative positions of the UVPD fragments postdissociation. This information is intended to be utilized for retrieving higher-order molecular structural details that include the conformation, subunit stoichiometry, and molecular interactions as well as to understand the dissociation dynamics of the MMAs in the gas phase.

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Section: Resultsmentioning

confidence: 99%

An Orbitrap/Time-of-Flight Mass Spectrometer for Photofragment Ion Imaging and High-Resolution Mass Analysis of Native Macromolecular Assemblies

Mathew

Giskes

Lekkas

et al. 2023

J. Am. Soc. Mass Spectrom.

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“…利用新的面阵式检出器, 有关面阵型成像的实验结果也逐步有了报道 [28,29,34] (图4 Figure 3 Schematic diagram of the VMI device using Tpx3Cam ultrafast light speed camera. MCP coupled with P47 phosphors, P47 will flash in response to ions impact on MCP.…”

Section: 面阵型Ims成像技术mentioning

confidence: 99%

Recent progress in mass spectrometry based molecular imaging

Yang¹,

Liu²,

Zhao³

et al. 2020

Sci. Sin.-Vitae

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Recent progress in gold nanoparticle-based biosensing and cellular imaging SCIENCE CHINA Chemistry 59, 783 (2016); Recent progress of bulk heterojunction solar cells based on small-molecular donors Chinese Science Bulletin 58, 2677 (2013); Recent advances in mass spectrometry based single cell analysis methods SCIENTIA SINICA Chimica 46, 133 (2016); Advances in mass spectrometry imaging and its application

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“…The additional impact position information provided by the TPX compared to conventional MS detectors has previously been utilized to obtain insight into the ion transport properties and ion optical processes within the MS, 14 , 16 , 18 , 20 as well as to improve the spatial resolution and throughput of MS imaging via stigmatic ion imaging. 7 , 9 , 10 , 13 , 15 , 19 The TPX detector used for previous studies was limited by a moderate time resolution (at best 10 ns) and single-stop detection for each pixel that can result in a low intensity of higher mass-to-charge ( m / z ) signals (longer flight time) due to the occupancy of pixels by earlier arriving ions. 1 …”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Imaging of High Mass Proteins and Metastable Fragments Using Matrix-Assisted Laser Desorption/Ionization, Axial Time-of-Flight Mass Spectrometry, and TPX3CAM

Mathew

Keelor²,

Eijkel

et al. 2022

Anal. Chem.

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The Timepix (TPX) is a position-and time-sensitive pixelated charge detector that can be coupled with time-of-flight mass spectrometry (TOF MS) in combination with microchannel plates (MCPs) for the spatially and temporally resolved detection of biomolecules. Earlier generation TPX detectors used in previous studies were limited by a moderate time resolution (at best 10 ns) and single-stop detection for each pixel that hampered the detection of ions with high mass-to-charge (m/z) values at high pixel occupancies. In this study, we have coupled an MCP-phosphor screen-TPX3CAM detection assembly that contains a silicon-coated TPX3 chip to a matrix-assisted laser desorption/ ionization (MALDI)-axial TOF MS. A time resolution of 1.5625 ns, per-pixel multihit functionality, simultaneous measurement of TOF and time-over-threshold (TOT) values, and kHz readout rates of the TPX3 extended the m/z detection range of the TPX detector family. The detection of singly charged intact Immunoglobulin M ions of m/z value approaching 1 × 10 6 Da has been demonstrated. We also discuss the utilization of additional information on impact coordinates and TOT provided by the TPX3 compared to conventional MS detectors for the enhancement of the quality of the mass spectrum in terms of signal-to-noise (S/N) ratio. We show how the reduced dead time and event-based readout in TPX3 compared to the TPX improves the sensitivity of high m/z detection in both low and high mass measurements (m/z range: 757−970,000 Da). We further exploit the imaging capabilities of the TPX3 detector for the spatial and temporal separation of neutral fragments generated by metastable decay at different locations along the field-free flight region by simultaneous application of deflection and retarding fields.

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Stigmatic imaging of secondary ions in MeV-SIMS spectrometry by linear Time-of-Flight mass spectrometer and the TimePix detector

Cited by 7 publications

References 34 publications

An Orbitrap/Time-of-Flight Mass Spectrometer for Photofragment Ion Imaging and High-Resolution Mass Analysis of Native Macromolecular Assemblies

An Orbitrap/Time-of-Flight Mass Spectrometer for Photofragment Ion Imaging and High-Resolution Mass Analysis of Native Macromolecular Assemblies

Recent progress in mass spectrometry based molecular imaging

Time-Resolved Imaging of High Mass Proteins and Metastable Fragments Using Matrix-Assisted Laser Desorption/Ionization, Axial Time-of-Flight Mass Spectrometry, and TPX3CAM

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