Stilbene Production by Vitis vinifera Cell Suspension Cultures: Methyl Jasmonate Induction and ¹³C Biolabeling

Abstract

Vitis vinifera cells grown in vitro respond to methyl jasmonate (MeJa) with enhancement of phytoalexin accumulation. MeJa added during the first half of the exponential growth phase increased the intracellular accumulation of stilbenes, mainly (Z)- and (E)-piceid (3,5,4‘-trihydroxystilbene-3-O-β-glucoside), in strains grown in either maintenance medium or polyphenol inductive medium. Plant cell cultures were used to produce isotopically (13C) labeled stilbenes by addition of 13C phenylalanine at different time intervals and in various concentrations. Incorporation of 13C phenylalanine into stilbenes was measured by EA-IRMS, and three successive 2-mM phenylalanine additions provided the best result (66%). This allowed production of 13C labeled Vitis vinifera phenolic compounds for investigation of their absorption in humans.

Response of Vitis vinifera cell cultures to Phaeomoniella chlamydospora: changes in phenolic production, oxidative state and expression of defence-related genes

“…Application of methyl jasmonate to the surface of grapevine leaves results in the formation of lesions that mimic the typical hypersensitive response, and in the activation of associated defence responses like increase of salicylic acid production, accumulation of phenolic compounds and expression of defence-related genes (Repka et al 2001). Moreover, elicitation of V. vinifera cell suspension cultures with methyl jasmonate results in phytoalexin accumulation, namely stilbene accumulation (Krisa et al 1999). In this work, methyl jasmonate induced the production of the same phenolics stimulated by P. chlamydospora elicitation.…”

Identification and functional characterization of grapevine transporters that mediate glucose-6-phosphate uptake into plastids

“…MeJA is an important plant regulator involved in fruit ripening, senescence, vegetative growth, cell cycle regulation, and plant defense mechanisms in response to herbivores, pathogen attack, and environmental stresses (Liechti and Farmer 2002;Wasternack and Hause 2002;Castillo and León 2008;Pauwels and Goossens 2011). The observed strong upregulation of VvPAL1 mediated by MeJA, is in line with previous reports showing that this hormone promotes the accumulation of several phenolic compounds including phytoalexins, anthocyanins, resveratrol and viniferins, and also induces sesquiterpene biosynthesis in grape cells (Krisa et al 1999;Righetti et al 2007;D'Onofrio et al 2009;Qu et al 2011;Santamaria et al 2011Santamaria et al , 2012. In addition, MeJA was shown to activate the biosynthesis of terpenoids in the leaves of Vitis cv.…”

Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

“…However, very low amounts of trans-resveratrol were detected in the spent medium when grapevine cell cultures were elicited only with MJ, and no trans-resveratrol was detected in cell cultures treated with ethanol, the solvent in which MJ is delivered to the culture (data not shown). In a similar way, Krisa et al (1999) described that the amount of total stilbenes secreted to the culture medium was negligible in both MJ and control cultures of three V. vinifera cultivars. These authors reported that piceid (glucosylated form of resveratrol) accumulation inside the grape cells of Cavernet-Sauvignon cultivar was notably induced when 25 µM MJ was added to the induction medium on day 6 (6.3 ± 0.2 mg piceid/g DW).…”

“…Analysis of trans-resveratrol production in untreated Vitis cell cultures (Teguo et al, 1996;Morales et al, 1998;Krisa et al, 1999;Tassoni et al, 2005) revealed a low level of trans-resveratrol accumulation, less than 0.01% DW or 2-3 mg/l. Therefore, various strategies such as the use of biotic and abiotic elicitors, addition of biosynthesis precursors and genetic transformation have been considered to improve the production of trans-resveratrol.…”