Stilbenes contribute to the anticancer effects of Rheum�undulatum L. through activation of apoptosis

Trinh, Tuy An; Lee, Dahae; Park, Seon Ju; Kim, Seung Hyun; Park, Jae Gyu; Kim, Ji Hwan; Kang, Ki Sung

doi:10.3892/ol.2019.9926

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…Trinh stated that aloe-emodin, chrysophanol, rhapontigenin, and rhaponticin were isolated from the RU extracts and their fractions [ 23 ]. Nevertheless, our study identified chrysophanol, emodin, and physcion to be the major compounds in the RU extracts.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species

Jargalsaikhan

Chen

et al. 2021

Molecules

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The Mongolian rhubarb—Rheum undulatum L. (RU)—and Rumex crispus L. (RC)—a Taiwanese local rhubarb belonging to the family of Polygonaceae—are principal therapeutic materials in integrative medicine due to their rich quantities of bioactive compounds; however, their phytochemical and antioxidant properties, and anti-cancer activity is poorly investigated. Furthermore, the phytochemical characteristics of both species may be affected by their different geographical distribution and climatic variance. The current study aimed to compare RU with RC extracts in different polarity solvents (n-hexane, ethyl acetate, acetone, ethanol, and water) for their phytochemical contents including the total phenolic content (TPC), total anthraquinone content (TAC), total flavonoid content (TFC), antioxidant and free radical scavenging capacities, and anticancer ability on the HepG2 cell. Except for the n-hexane extract, all of the RU extracts had considerably higher TPCs than RC extracts, ranging from 8.39 to 11.16 mg gallic acid equivalent (GAE) per gram of dry weight, and the TPCs of each extract were also significantly correlated with their antioxidant capacities by ABTS, DPPH, and FRAP assays (p < 0.05). Moreover, there was no remarkable association between the antioxidant capacities and either TACs or TFCs in both the RU and RC extracts. Besides, high-performance liquid chromatography (HPLC) analysis revealed that both the RU and RC extracts contained chrysophanol, emodin, and physcion, and those bioactive compounds were relatively higher in the n-hexane solvent extracts. Additionally, we observed different levels of dose-dependent cytotoxic effects in all the extracts by cell viability assay. Notably, the ethanol extract of RU had a compelling cytotoxic effect with the lowest half-maximum inhibition concentration (IC50-171.94 ± 6.56 µg/mL at 48 h) among the RU extracts than the ethanol extract of RC. Interestingly, the ethanol extract of RU but not RC significantly induced apoptosis in the human liver cancer cell line, HepG2, with a distinct pattern in caspase-3 activation, resulting in increased PARP cleavage and DNA damage. In summary, Mongolian Rhubarb, RU, showed more phytochemical contents, as well as a higher antioxidant capacity and apoptotic effect to HepG2 than RC; thus, it can be exploited for the proper source of natural antioxidants and liver cancer treatment in further investigation.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species

Jargalsaikhan

Chen

et al. 2021

Molecules

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“…The exposure of these cells to aloe-emodin or chrysophanol 1-O-b-D-glucopyranoside increased the poly(ADP-ribose)polymerase (PARP) cleavage and induced downregulation of the anti-apoptotic protein, Bcl-2. Additionally, chrysophanol 1-O-b-D-glucopyranoside slightly stimulated the expression of proapoptotic proteins such as Bid and Bax (Trinh et al 2019). In the MCF-7 human breast cell line, aloeemodin and rhapontigenin induced the mitochondriaindependent apoptosis (mediated by the caspase-8 pathway), while chrysophanol 1-O-b-D-glucopyranoside, acted by the mitochondria-dependent apoptotic pathway (Lee et al 2018).…”

Section: Anticancer Activitymentioning

confidence: 99%

Rheum rhaponticum and Rheum rhabarbarum: a review of phytochemistry, biological activities and therapeutic potential

Kołodziejczyk-Czepas

Liudvytska

2020

Phytochem Rev

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The Rheum genus (Polygonaceae) covers about 60 species of rhubarbs, including specimens with a long ethnomedicinal history in Asia, Europe and other regions of the world. The work reviews available literature (until March, 2020) on phytochemical profile, ethnomedicinal recommendations, biological activities, pharmacological uses and future prospects for therapeutic applications of Rheum rhabarbarum L. (garden rhubarb) and Rheum rhaponticum L. (rhapontic rhubarb). Although the above species are well-known vegetables, scientific interest in these plants is a relatively new issue; most of evidence of their biological activities and therapeutic potential derives from the last 15 years. Rhubarbs contain numerous bioactive substances, belonging to diverse groups of phytochemicals, e.g. stilbenes, anthraquinones and flavonoids. The registered special extract of R. rhaponticum (ERr731®) is administered to alleviate the menopause-related complaints. Furthermore, both ethnomedicinal surveys and recent studies on bioactive substances from rhubarbs indicate that these plants may have significantly broader range of beneficial effects such as antioxidant, anti-inflammatory, antimicrobial and cardioprotective activities.

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“…Տերևակոթունները պարունակում են մինչև 4,5% ածխաջրեր, 3,5% օրգանական թթուներ (խնձորաթթու, օքսալաթթու, կիտրոնաթթու), անտրաքինոններ, անտրոններ, ստիլբեններ, ֆլավոնոիդներ, գլիկոզիդներ, դաբաղանյութեր, վիտամիններ, β-կարոտին, կալիում, կալցիում, ֆոսֆոր, սելեն, մագնեզիում և այլն [7,10,13,16,21]:…”

Section: Rheum Rhabarbarum Lsoilless Culturemonosaccharidesoxalic Acidunclassified

“…Խավրծիլը հայտնի է ասիական, եվրոպական և այլ մշակույթների ավանդական բժշկության [4], այուվերդյան բժշկության մեջ [9], իսկ Իրանում կիրառվում է մաշկի գերպիգմենտացիայի բուժման ժամանակ [11]: Այն ունի հիպոլիպիդեմիկ ազդեցություն, օգտագործվում է ճարպակալման դեմ, նաև հակաքաղցկեղածին է [17,21]:…”

Section: Rheum Rhabarbarum Lsoilless Culturemonosaccharidesoxalic Acidunclassified

Խավրծիլի Աճեցման Հնարավորությունը Բացօթյա Հիդրոպոնիկայում ԵՒ Հողում

Թադեւոսյան¹,

Բաղդասարյան²,

Ստեփանյան³

et al. 2022

Biological Journal of Armenia

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Առաջին անգամ ուսումնասիրվել են խավրծիլի մշակման հնարավորությունն ու արդյունավետությունը բացօթյա հիդրոպոնիկայի և հողային պայմաններում: Պարզվել է, որ խավրծիլի աճեցումը Արարատյան դաշտի անհող պայմաններում հնարավոր է, այն արդյունավետ է և հեռանկարային: Հաստատվել է

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Stilbenes contribute to the anticancer effects of Rheum�undulatum L. through activation of apoptosis

Cited by 4 publications

References 26 publications

Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species

Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species

Rheum rhaponticum and Rheum rhabarbarum: a review of phytochemistry, biological activities and therapeutic potential

Խավրծիլի Աճեցման Հնարավորությունը Բացօթյա Հիդրոպոնիկայում ԵՒ Հողում

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