Stimulation of Adenylate Cyclase in the Thyroid

Marshall, Nicholas J.

doi:10.1007/978-1-4613-4322-6_7

Cited by 6 publications

(3 citation statements)

References 116 publications

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“…Long-acting thyroid stimulator-immunoglobulin, which is believed to act through a cyclic nucleotide mechanism (Marshall, 1976), supported follicular architecture, promoted iodideuptake and induced acute depolarization analogous to that seen with TSH. Surprisingly, this effect was readily reversible on removal of the LATS-IgG, a phenomenon which has also been reported by Konno & McKenzie (1970).…”

Section: Porcine Cell Culturementioning

confidence: 44%

Changes in Membrane Potential of Cultured Porcine and Human Thyroid Cells in Response to Thyrotrophin and Other Agents

Bourke¹,

Carseldine²,

Ferris³

et al. 1981

Journal of Endocrinology

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Thyrotrophin (TSH), cyclic AMP, cyclic GMP and 1-methyl-3-isobutyl-xanthine (MIX) promoted the reassociation of isolated porcine and human thyroid cells into follicular structures in culture and stimulated the uptake of radio-iodide. Monolayer cells were present in all cultures, but in decreasing proportions as the concentration of stimulator was increased. The resting membrane potential of porcine thyroid cells cultured for 4 days in the presence of TSH was -54 +/- 3.6 (mean +/- S.D.) mV for follicular cells and -31 +/- 2.6 mV for monolayer cells. In the absence of TSH, only monolayer cells were present and their membrane potential was -24 +/- 2.0 mV. Removal of hormone by washing resulted in hyperpolarization to -70 +/- 2.9 mV (follicular cells) or -59 +/- 3.4 mV (monolayer cells). Subsequent replacement of TSH, or addition of cyclic AMP, MIX, prostaglandin E1 (PGE1) or long-acting thyroid stimulator immunoglobulin resulted in depolarization of previously hyperpolarized cells, to approximately the membrane potential observed before washing. Incubation in MIX resulted in enhanced sensitivity to the depolarizing effect of TSH. Cells cultured in the absence of TSH were unresponsive to TSH or other stimulators. The membrane potential of human thyroid cells behaved similarly in response to TSH, to hormone removal and replacement, and to MIX and PGE1.

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Section: Porcine Cell Culturementioning

confidence: 44%

Changes in Membrane Potential of Cultured Porcine and Human Thyroid Cells in Response to Thyrotrophin and Other Agents

Bourke¹,

Carseldine²,

Ferris³

et al. 1981

Journal of Endocrinology

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“…The biological responses of in-vitro preparations of the thyroid gland have received extensive investigation, and in particular, the effect of thyrotrophin (TSH) on the thyroidal adenylate cyclase-cyclic AMP system has been the subject of detailed studies in membrane (review: Marshall, 1976), slice (Van Sande & Dumont, 1973;Holmes, Dirmikis, Martin & Munro, 1978) and isolated cell preparations (Takasu, Charrier, Mauchamp & Lissitzky, 1977;Rapoport & Adams, 1978). Stimulation of cyclic AMP accumulation in thyroid slices in vitro has also been observed with thyroid-stimulating antibodies (TSAb) (Onaya, Kotani, Yamada & Ochi, 1973;McKenzie & Zakarija, 1976;Holmes et al 1978); these are thought to interact with the TSH receptor in human thyroid tissue, stimulate adenylate cyclase and thyroid hormone production, and be associated with the thyrotoxicosis of Graves's disease.…”

Section: Introductionsupporting

confidence: 40%

Inhibition by Normal Immunoglobulins of Thyrotrophin-Stimulated Production of Cyclic Amp in Slices of Normal Human Thyroid

Bidey¹,

Marshall²,

Rp³

1980

Journal of Endocrinology

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Slice preparations of normal human thyroid tissue have been used to investigate the effect of normal immunoglobulin G (IgG) on thyrotrophin (TSH)-induced accumulation of cyclic AMP. Incubation of slices in the presence of both TSH and normal IgG for 20 min reduced the stimulation of cyclic AMP accumulation elicited by TSH alone by approximately 30%. However, preincubation of slices with IgG for 100 min before addition of TSH virtually abolished the response to TSH. The latter effect of normal IgG was reversible, and removal of IgG before exposure to TSH allowed an unimpaired cyclic AMP response to TSH. The implications of these observations with respect to the application of this system to the functional bio-detection of thyroid-stimulating antibodies in IgG fractions from thyrotoxic sera are discussed.

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“…The effect of thyrotrophin (TSH) on adenylate cyclase activation and cyclic AMP accumulation in in vitro preparations of the human thyroid has been the subject of extensive investigation in plasma membranes (Marshall 1976;Holmes et al 1978;Bech & Nistrup-Madsen 1978), tissue slices (Holmes et al 1978;Onaya et al 1973;Takasu et al 1976;McKenzie & Zakarija 1978;Bidey et al 1980Bidey et al , 1981a and, more recently, in isolated thyroid cells maintained as monolayer cell cultures (Rapoport et al 1974;Kaneko 1976;Toccafondi et al 1980;Bidey et al 1981b;Hinds et al 1981;Stockleetal. 1981).…”

mentioning

confidence: 42%

A characterisation of cyclic AMP release from monolayer cultures of normal human thyroid cells

Bidey

Marshall

Ekins

1982

Acta Endocrinologica

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The thyrotrophin (TSH)-dependent and time-related release of cyclic AMP has been characterised in primary monolayer cultures of normal human thyroid cells. Accumulation of cyclic AMP within the incubation medium was detectable within 1 h of exposure of cultures to 5 mU TSH/ml, and increased throughout of subsequent 15 h incubation period, final levels attained being consistently in excess of the corresponding intracellular cyclic AMP levels. Accumulation of cyclic AMP in the incubation medium was dependent on TSH dose, for both short (1 h) and prolonged (16 h) incubations. Moreover, after incubation for 16 h, cyclic AMP levels in the incubation medium were significantly (P < 0.01) in excess of intracellular levels for each dose of TSH tested above 0.2 mU/ml. In the absence of TSH, accumulation of cyclic AMP in the incubation medium remained low, after both 1 h and 16 h incubation periods.A consideration of these observations suggests that a bioassay based upon the cyclic AMP content of incubation medium samples should provide a more precise detection system for thyroid stimulators than those measuring the intracellular cyclic AMP response, and this has been demonstrated for a cell preparation in which the intracellular response to TSH was minimal.

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Stimulation of Adenylate Cyclase in the Thyroid

Cited by 6 publications

References 116 publications

Changes in Membrane Potential of Cultured Porcine and Human Thyroid Cells in Response to Thyrotrophin and Other Agents

Changes in Membrane Potential of Cultured Porcine and Human Thyroid Cells in Response to Thyrotrophin and Other Agents

Inhibition by Normal Immunoglobulins of Thyrotrophin-Stimulated Production of Cyclic Amp in Slices of Normal Human Thyroid

A characterisation of cyclic AMP release from monolayer cultures of normal human thyroid cells

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