Stimulation of adrenal cortisol biosynthesis by epidermal growth factor

Singh-Asa, P.; Waters, Michael J.

doi:10.1016/0303-7207(83)90047-3

Cited by 37 publications

(10 citation statements)

References 26 publications

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“…Epidermal growth factor may also be acting in¬ directly on the ovary. Defleecing doses of EGF increase plasma cortisol production and depress plasma thyroxine concentrations (Singh-Asa & Waters, 1983;Waters et al 1984). A number of studies have demonstrated the importance of adrenal and thyroid hormones in ovarian function.…”

Section: Discussionmentioning

confidence: 97%

Effect of epidermal growth factor on reproductive function of ewes

Shaw¹,

Jorgensen²,

Tweedale³

et al. 1985

Journal of Endocrinology

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Adult Merino ewes were infused via the jugular vein with either saline (n = 5) or epidermal growth factor (EGF) (4.2 micrograms/kg per h, n = 6) for 24 h in either the luteal phase or the follicular phase of the oestrous cycle and reproductive function was examined. Infusion of EGF during the luteal phase caused no detectable change in plasma progesterone or prolactin concentrations over a 7-day period compared with the controls. Infusion of EGF during the follicular phase suppressed the oestrous rise in plasma oestradiol. Luteinizing hormone pulse amplitude was increased and pulse frequency was decreased by the end of the infusion. All control ewes had a pro-oestrous LH surge and mated, but the LH surge and oestrus were prevented by EGF infusion. Nevertheless, plasma progesterone levels rose subsequently in the EGF-infused ewes in parallel with the control ewes, suggesting that the preovulatory follicle had luteinized. Both LH and FSH rose over the 7 days after EGF infusion to levels similar to those in ovariectomized ewes. Thus EGF appears to inhibit follicular oestradiol production, although it does not affect luteal progesterone production or follicular luteinization. We suggest that the alteration in gonadotrophin secretion patterns results from a disturbance of feedback mechanisms between the ovary and the hypothalamopituitary axis, although a direct effect in the brain or the pituitary gland cannot yet be excluded.

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Section: Discussionmentioning

confidence: 97%

Effect of epidermal growth factor on reproductive function of ewes

Shaw¹,

Jorgensen²,

Tweedale³

et al. 1985

Journal of Endocrinology

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“…Shortterm exposure of bovine zona glomerulosa cells to IGF-I, while stimulating DNA synthesis, failed to alter aldosterone secretion (Horiba et al, 1987). EGF affects cortisol secretion in sheep (Singh-Asa & Waters, 1983). It was subsequently found that mEGF (10-100 ng/ml) increased cortisol secretion by sheep adrenocortical cells in vitro (Singh-Asa et af., 1985).…”

Section: Growth Factormentioning

confidence: 98%

Regulation of Steroid Synthesis and Metabolism by Growth Factors

Reed

James

1989

Clinical Endocrinology

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“…Previous studies of bovine (Singh & Waters 1983) and sheep (Singh et al 1985) adrenal cortical cells demonstrated that the addition of EGF to culture media resulted in stimulated cortisol secretion from and cholesterol synthesis in the cells, without significant effect on the production rate of steroid intermediates. Abolition of the EGF-mediated stimulation of cortisol secretion by inhibitors of cholesterol synthesis led to the idea that HMG-CoA (A) H295R cells were stimulated with EGF (10 ng/ml) or bFGF (10 ng/ml) in the presence or absence of CHX (10 g/ml) for 24 h. Total RNAs were isolated and subjected to northern blot analysis as described in Materials and Methods.…”

Section: Discussionmentioning

confidence: 96%

“…Singh and coworkers reported that EGF stimulated cortisol secretion from cultured bovine (Singh & Waters 1983) and sheep (Singh et al 1985) adrenal cortical cells. The stimulation was abolished when inhibitors of cholesterol biosynthesis, compactin and AY9944, were added.…”

Section: Introductionmentioning

confidence: 99%

Differential regulation of 3beta-hydroxysteroid dehydrogenase type II and 17alpha-hydroxylase/lyase P450 in human adrenocortical carcinoma cells by epidermal growth factor and basic fibroblast growth factor

Doi¹,

Takemori²,

Ohta³

et al. 2001

Journal of Endocrinology

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Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) are pluripotent growth factors that stimulate both the proliferation and steroidogenesis of adrenocortical cells. Here we demonstrate that EGF and bFGF specifically induce mRNA of 3 -hydroxysteroid dehydrogenase type II (3 HSD II) and suppress that of 17 -hydroxylase/ lyase P450 (CYP17) in human adrenocortical H295R cells. The induction of 3 HSD II mRNA did not occur until 6 h after the growth factor treatment and was completely abolished in the presence of a protein synthesis inhibitor, cycloheximide (CHX), suggesting that the induction required de novo protein synthesis. The CYP17 mRNA suppression began at almost the same time as the induction of the 3 HSD II mRNA. Interestingly, the CYP17 mRNA level was increased by the CHX treatment. Both the 3 HSD II and CYP17 mRNAs were repressed by treatment with a calmodulin kinase II (CaMK II) inhibitor, KN-93, and were enhanced by a mitogenactivated protein kinase (MAPK) inhibitor, PD98059. The PD98059-mediated induction of the 3 HSD II mRNA was completely blocked by the CHX treatment. Interestingly, treatment with EGF in the presence of both PD98059 and CHX produced a greater increase in the CYP17 mRNA than did treatment in the presence of PD98059 alone. These results suggest that CHX-sensitive factor(s) and CaMK II-and MAPK-signaling pathways may have important roles in both induction of 3 HSD II and suppression of CYP17 by EGF or bFGF in H295R cells.

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Stimulation of adrenal cortisol biosynthesis by epidermal growth factor

Cited by 37 publications

References 26 publications

Effect of epidermal growth factor on reproductive function of ewes

Effect of epidermal growth factor on reproductive function of ewes

Regulation of Steroid Synthesis and Metabolism by Growth Factors

Differential regulation of 3beta-hydroxysteroid dehydrogenase type II and 17alpha-hydroxylase/lyase P450 in human adrenocortical carcinoma cells by epidermal growth factor and basic fibroblast growth factor

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