Stimulation of anion secretion by β‐adrenoceptors in the mouse endometrial epithelium

Abstract

Regulation of anion secretion by adrenoceptors in primary culture of mouse endometrial epithelium was investigated using the short circuit current (ISC) technique. Adrenaline stimulated a sustained increase in the ISC in a concentration‐dependent manner. The adrenaline‐induced ISC could be inhibited by pretreatment with diphenylamine 2,2'‐dicarboxylic acid (DPC) or replacement of external Cl− and HCO3−, but not by amiloride or replacement of Na+ in apical solution. The concentration‐dependent responses of the adrenaline‐induced ISC to the CF channel blockers glibenclamide and DPC were examined and exhibited IC50 values of 380 and 960 μm, respectively. The effect of various adrenoceptor agonists on the ISC was examined. The order of potency appeared to be isoprenaline > adrenaline > noradrenaline, while no response was elicited by the α‐adrenoceptor agonist methoxamine, indicating a predominant involvement of β‐adrenoceptors. The β‐adrenoceptor antagonist propranolol was found to be much more effective than the α‐adrenoceptor antagonist phentolamine in inhibiting the ISC responses induced by all adrenoceptor agonists examined. The effect of adrenaline on the ISC was mimicked by an adenylate cyclase activator, forskolin, but suppressed by the adenylate cyclase inhibitor MDL 12,330A, indicating the involvement of cAMP. Our results demonstrate that anion secretion by the mouse endometrial epithelium is regulated by β‐adrenoceptors and involves a cAMP‐dependent mechanism.

Suppression of CFTR-Mediated Cl− Secretion by Enhanced Expression of Epithelial Na+ Channels in Mouse Endometrial Epithelium

“…Previous studies have shown that cultured endometrial epithelia exhibit a basal Isc which is predominantly amiloride-sensitive, indicative of Na ϩ absorption which could be switched to predominant CFTRmediated anion secretion upon stimulation (10,16,17). In the present study, variations in basal Isc were observed for different batches of cultured endometrial epithelia.…”

Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E2

“…In cultured mouse endometrial cells, adrenergic agonists were shown to produce an increase in Isc that was consistent with anion secretion. This response was mediated by â-adrenergic receptors and was blocked by apical addition of either glibenclamide or diphenylamine 2,2'-dicarboxylic acid (DPC) (Chan, Fong, Chung & Wong, 1997). These experiments indicate that endometrial epithelial cells in culture are capable of both Na¤ absorption and anion secretion.…”

Inhibition of amiloride-sensitive Na + absorption by activation of CFTR in mouse endometrial epithelium

“…Recent studies on primary cultures of mouse endometrial epithelium have demonstrated predominantly Na + absorption under basal conditions but mostly anion secretion upon stimulation [4,5,6,9]. Cultured endometrial epithelium responds to adrenaline, noradrenaline, prostaglandin E 2 (PGE 2 ) or forskolin with an increase in the short-circuit current (I sc ) that can be attributed predominantly to Cl -and HCO 3 -secretion.…”