Stimulation of craniofacial and intramedullary bone formation by negatively charged beads

Krukowski, Marilyn; Shively, Robert A.; Osdoby, Philip; Eppley, Barry L.

doi:10.1016/0278-2391(90)90233-r

Cited by 55 publications

(31 citation statements)

References 19 publications

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“…Indeed, changes in surface energy, charge, and composition increase tissue adhesion 2,3 and integration, 4,5 and alterations in surface geometry and roughness enhance osteoblastic function 6,7 and response to hormones. 8 Moreover, deposition of a calcium phosphate layer on the Ti surface increases implant integration into surrounding tissues.…”

Section: Introductionmentioning

confidence: 99%

RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

Secchi

Grigoriou

Shapiro³

et al. 2007

J Biomedical Materials Res

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. (2007). RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis. Retrieved from http://repository.upenn.edu/be_papers/101 RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis Abstract A major cause of implant failure in skeletal tissues is failure of osseointegration, often due to lack of adhesion of cells to the titanium (Ti) alloy interface. Since arginine-glycine-aspartic acid (RGD)-containing peptides have been shown to regulate osteoblast adhesion, we tested the hypothesis that, bound to a Ti surface, these peptides would promote osteoblasts differentiation, while at the same time inhibit apoptosis. RGDS and RGES (control) peptides were covalently linked to Ti discs using an APTS linker. While the grafting of both RGDS and RGES significantly increased Ti surface roughness, contact angle analysis showed that APTS significantly increased the surface hydrophobicity; when the peptides were tethered to Ti, this was reduced. To evaluate attachment, MC3T3-E1 osteoblast cells were grown on these discs. Significantly more cells attached to the Ti-grafted RGDS then the Ti-grafted RGES control. Furthermore, expression of the osteoblasts phenotype was significantly enhanced on the Ti-grafted RGDS surface. When cells attached to the Ti-grafted RGDS were challenged with staurosporine, an apoptogen, there was significant inhibition of apoptosis; in contrast, osteoblasts adherent to the Ti-grafted RGES were killed. It is concluded that RGDcontaining peptides covalently bonded to Ti promotes osteoblasts attachment and survival with minimal changes to the surface of the alloy. Therefore, such modifications to Ti would have the potential to promote osseointegration in vivo. Abstract: A major cause of implant failure in skeletal tissues is failure of osseointegration, often due to lack of adhesion of cells to the titanium (Ti) alloy interface. Since arginine-glycine-aspartic acid (RGD)-containing peptides have been shown to regulate osteoblast adhesion, we tested the hypothesis that, bound to a Ti surface, these peptides would promote osteoblasts differentiation, while at the same time inhibit apoptosis. RGDS and RGES (control) peptides were covalently linked to Ti discs using an APTS linker. While the grafting of both RGDS and RGES significantly increased Ti surface roughness, contact angle analysis showed that APTS significantly increased the surface hydrophobicity; when the peptides were tethered to Ti, this was reduced. To evaluate attachment, MC3T3-E1 osteoblast cells were grown on these discs. Significantly more cells attached to the Ti-grafted RGDS then the Ti-grafted RGES control. Furthermore, expression of the osteoblasts phenotype was significantly enhanced on the Ti-grafted RGDS surface. When cells attached to the Ti-grafted RGDS were challenged with staurosporine, an apoptogen, there was significant inhibition of apoptosis; in contrast, osteoblasts adherent to the Ti-gra...

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Section: Introductionmentioning

confidence: 99%

RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

Secchi

Grigoriou

Shapiro³

et al. 2007

J Biomedical Materials Res

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“…Most of the investigations have focused on the negatively charged Sephadex resin (NCDR) because with few exceptions (vide infra), it promotes bone formation and repair. [8][9][10][11] The osteotropic effect of NCDR may be mediated by the local enhancement of transforming growth factor-␤ production, 11 which could also stimulate osteoprogenitor cell proliferation. NCDR does not appear to alter the normal patterns of bone cell proliferation in vitro.…”

Section: Discussionmentioning

confidence: 99%

Positively charged dextran resin inhibits trabecular bone repair in the rabbit tibial physis

Cobos

Yang

Zhang

et al. 1998

J. Biomed. Mater. Res.

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Because exposure to positively charged dextran resin (PCDR) inhibits the growth of cultured rat and human bone cells, we tested the hypothesis that PCDR might inhibit bone repair in vivo. Central physeal defects were created by drilling 3.0-mm holes from the proximal tibial plateau into the metaphysis. The defects in left tibiae were packed with neutral resin (control); those in right tibiae were filled with PCDR. At the end of the 1st, 2nd, 3rd, and 10th postoperative weeks, the outcomes were quantitated by documenting the percent trabecular bone volume within the defect. The PCDR-filled defects showed a significant decrease in trabecular bone formation as early as the 2nd week. By the 10th postoperative week, formation of trabeculae had been reduced by nearly 40%. The inhibition conferred by PCDR suggests that the resin could be used as a suppressive interpositional material.

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“…Previous reports have indicated that charged dextran resins can alter the behavior of the mesenchymal osteogenic precursor elements within marrow, 1,2 periosteum, 2,3 and dura. 4 The osteotropic effect of negatively charged resin finds its clinical parallel in the way endogenous and imposed cathodal potentials stimulate bone cell differentiation and function.…”

Section: Introductionmentioning

confidence: 99%

Antitumor effect of positively charged resin in the hamster cheek pouch model

Simmons

Seitz

Cooper

et al. 1997

J. Biomed. Mater. Res.

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Following the signal observation that contact with positively charged dextran resin (PCDR) inhibited the growth of cultured mammary (Hs578T and MDA-MB-231), pancreatic (H2T), and myeloma (RR-658) tumor cell lines, studies were developed in the hamster cheek pouch model using hamster H2T pancreatic tumor cells to determine if the antiproliferative effect of PCDR could inhibit tumorigenesis. In these studies, the control population represented groups injected with H2T cells alone or in combination with either neutral or negatively charged resin. When cells (5 x 10(2) to 1 x 10(5)) and PCDR were administered simultaneously, the tumor incidence (percent engraftment) and growth of tumors that already had been established were significantly reduced. When PCDR was injected into already established 1-35-mm2 H2T tumors (engraftment for 21 days = 96%), the resin suppressed the growth of the smallest tumors (< 10 mm2). In none of these trials was the somatic growth of the host hamsters affected. PCDR contact with H2T cells in vitro for 4 days or used to treat growing solid tumors for 72 days significantly reduced cellular ornithine decarboxylase activity. While the mechanism of PCDR action has not been established, the observations have implications for in vivo tumor therapeutic models.

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Stimulation of craniofacial and intramedullary bone formation by negatively charged beads

Cited by 55 publications

References 19 publications

RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

Positively charged dextran resin inhibits trabecular bone repair in the rabbit tibial physis

Antitumor effect of positively charged resin in the hamster cheek pouch model

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