Stimulation of electricity production in microbial fuel cells via regulation of syntrophic consortium development

Toczyłowska-Mamińska, Renata; Pielech‐Przybylska, Katarzyna; Sekrecka-Belniak, Anna; Dziekońska‐Kubczak, Urszula

doi:10.1016/j.apenergy.2020.115184

Cited by 24 publications

(3 citation statements)

References 45 publications

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“…In addition to the critical design components of MFCs, the most important component is the biocatalyst. Pure microbial cultures, as well as their synthetic or natural consortia, are fundamental for the operation and performance of MFCs as well as for the acquisition of certain products from their metabolism, e.g., electricity or particular metabolic pathways [34,35]. Similarly, the high-throughput approach for investigating the effects of electrode potential and external loads to control microbial metabolism is crucial for understanding MFC operation and community development [36,37].…”

Section: Introductionmentioning

confidence: 99%

High-throughput 96-well bioelectrochemical platform for screening of electroactive microbial consortia

Szydlowski

Ehlich

Goryanin

et al. 2022

Chemical Engineering Journal

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The development of bioelectrochemical systems reinforces the necessity for the identification and engineering of electroactive bacteria with improved performance and novel biochemical properties. In this study, using a newly designed 96-well-plate array of microbial fuel cells (MFCs), we compared the electroactive capabilities of microbial communities derived from four mine drainages. The maximum power density of individual wells after two weeks of inoculation was 102 mW/m 3 , whereas the maximum current density was 1.6 A/m 3 . Transferring communities from individual wells into larger MFCs comprising low (20 mg/L) and high (200 mg/L) concentrations of Cu yielded maximum power densities of 445 and 58 mW/m 3 , respectively, with up to a 3.7 fold decrease in Cu 2+ ions within 24 h. Electrochemical data analysis revealed that microbial consortia can be distinguished based on their electrochemical profiles. Our results showed that a 96-well MFC array is a suitable platform for high-throughput screening, selection, and subsequent source of enriched electroactive consortia. The quantitative and comparative analysis followed by principal component analysis indicated that the initial environmental conditions, as well as physical and chemical parameters of the lakes were crucial to develop an efficient electroactive community. Further applications of the proposed platform include genetic engineering, phenotype screening, and mutagenesis studies of both microbial communities and single cultures. This is the first time that a high-throughput MFC platform is used to evaluate the performance of multiple electroactive consortia towards Cu removal.

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Section: Introductionmentioning

confidence: 99%

High-throughput 96-well bioelectrochemical platform for screening of electroactive microbial consortia

Szydlowski

Ehlich

Goryanin

et al. 2022

Chemical Engineering Journal

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“…The effectiveness of BES also can be limited by microbial electron generation and transfer Table S2 indicates that microbial richness and diversity were highest in the MFC using bio-M/CG as the cathode catalyst, while using the abio-M/CG catalyst gave the opposite trend.…”

Section: Resultsmentioning

confidence: 99%

Biogenic Melanin-Modified Graphene as a Cathode Catalyst Yields Greater Bioelectrochemical Performances by Stimulating Oxygen–Reduction and Microbial Electron Transfer

Wu,

Liang,

Zhou

et al. 2023

ACS EST Water

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Bioelectrochemical systems (BES) can recover energy from organic-bearing waste streams, but their use has been stymied by poor electron transfer from the cathode. Redoxactive electron shuttles could stimulate electron transfer provided that they are compatible with the exoelectrogenic bacteria. This work evaluated melanin-modified carboxylated graphene (M/CG) as a novel cathode catalyst in a microbial fuel cell. Biogenic melanin catalysts (i.e., bio-M/CG) significantly increased bioelectricity production due to its abundant pyrrole N, which lowered chargetransfer resistance and, thus, promoted the cathodic oxygen− reduction reaction (ORR). The high content of pyrrole N in the bio-M/CG catalyst also enriched exoelectrogens, such as Azospirillum, Chryseobacterium, and Azoarcus, which accounted for over 50% of the total abundance of bacteria in biofilms on the anode. Moreover, the functional genes of key enzymes involved in microbial electron transfer (MET) were increased by the bio-M/CG catalyst. These data confirm that the bio-M/CG catalyst improved the bioelectrochemical performance via synergetic promotion of cathodic ORR and microbial electron transfer, thus providing a new alternative for advancing BES technology. This work highlights the potential application of melanin in enhancing cathodic oxygen−reduction reaction kinetics and improving microbial electron transfer in BES. This study emphasizes the promising application of melanin in enhancing the ORR kinetics and improving MET in BES, offering exciting prospects for future sustainable and environmentally friendly applications.

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“…ELMs have applications in both electrolytes and electrodes, such as the use of nanomaterials with high conductivity and chemical stability in combination with microorganisms, which can significantly improve the energy density and charging speed of batteries. − They provide high ion conductivity and better chemical stability, thereby reducing energy loss during battery operation. For example, using conjugated polyelectrolyte CPE-K as a conductive substrate, a living bioelectrochemical composite material that electronically connects a 3D network of Shewanella MR-1 with a gold electrode can increase the biocurrent to 150 times that of a control biofilm .…”

Section: Applications Of Elmsmentioning

confidence: 99%

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Lu,

Huang,

Cui

et al. 2024

ACS Synth. Biol.

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Engineered Living Materials (ELMs) are materials composed of or incorporating living cells as essential functional units. These materials can be created using bottom-up approaches, where engineered cells spontaneously form welldefined aggregates. Alternatively, top-down methods employ advanced materials science techniques to integrate cells with various kinds of materials, creating hybrids where cells and materials are intricately combined. ELMs blend synthetic biology with materials science, allowing for dynamic responses to environmental stimuli such as stress, pH, humidity, temperature, and light. These materials exhibit unique "living" properties, including self-healing, self-replication, and environmental adaptability, making them highly suitable for a wide range of applications in medicine, environmental conservation, and manufacturing. Their inherent biocompatibility and ability to undergo genetic modifications allow for customized functionalities and prolonged sustainability. This review highlights the transformative impact of ELMs over recent decades, particularly in healthcare and environmental protection. We discuss current preparation methods, including the use of endogenous and exogenous scaffolds, living assembly, 3D bioprinting, and electrospinning. Emphasis is placed on ongoing research and technological advancements necessary to enhance the safety, functionality, and practical applicability of ELMs in real-world contexts.

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Stimulation of electricity production in microbial fuel cells via regulation of syntrophic consortium development

Cited by 24 publications

References 45 publications

High-throughput 96-well bioelectrochemical platform for screening of electroactive microbial consortia

High-throughput 96-well bioelectrochemical platform for screening of electroactive microbial consortia

Biogenic Melanin-Modified Graphene as a Cathode Catalyst Yields Greater Bioelectrochemical Performances by Stimulating Oxygen–Reduction and Microbial Electron Transfer

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

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