Stimulation of Hyaluronan Synthetase by Platelet-Derived Growth Factor bb in Human Prostate Smooth Muscle Cells

Pullen, Mark; Thomas, Kerrie L.; Wu, Hsiao-Ling; Nambi, Ponnal

doi:10.1159/000056079

Cited by 9 publications

(9 citation statements)

References 22 publications

(20 reference statements)

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“…HA is found in prostate ECM in both benign and malignant pathology (De Klerk 1983; Pullen et al 2001; Misra et al 2006; Simpson and Lokeshwar 2008). HA size has differential effects on cell behavior that relate to its chain length: newly synthesized HA has a high MW(up to 2×10 4 kDa) and can inhibit a variety of cellular processes (Goldberg and Toole 1987; Bharadwaj et al 2007; Tian et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Far less is known about the influence of HA on prostate hyperplasia. An increase in total HA expression and variability of HA size is a feature of benign prostate disease (De Klerk 1983; Goulas et al 2000; Pullen et al 2001). Given the complex nature of prostate ECM, the use of ECM extracts is particularly advantageous for studies of specific components, such as HA, that influence the behaviors of prostate epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

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Hyaluronan in aged collagen matrix increases prostate epithelial cell proliferation

Damodarasamy

Vernon

Chan

et al. 2014

In Vitro Cell.Dev.Biol.-Animal

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The extracellular matrix (ECM) of the prostate, which is comprised primarily of collagen, becomes increasingly disorganized with age, a property that may influence the development of hyperplasia and cancer. Collageous ECM extracted from the tails of aged mice exhibits many characteristics of collagen in aged tissues, including the prostate. When polymerized into a 3-dimensional (3D) gel, these collagen extracts can serve as models for the study of specific cell-ECM interactions. In the present study, we examined the behaviors of human prostatic epithelial cell lines representing normal prostate epithelial cells (PEC), benign prostatic hyperplasia (BPH-1), and adenocarcinoma (LNCaP) cultured in contact with 3D gels made from collagen extracts of young and aged mice. We found that proliferation of PEC, BPH-1, and LNCaP cells were all increased by culture on aged collagen gels relative to young collagen gels. In examining age-associated differences in the composition of the collagen extracts, we found that aged and young collagen had a similar amount of several collagen-associated ECM components, but aged collagen had a much greater content of the glycosaminoglycan hyaluronan (HA) than young collagen. The addition of HA (of similar size and concentration to that found in aged collagen extracts) to cells placed in young collagen elicited significantly increased proliferation in BPH-1 cells, but not in PEC or LNCaP cells, relative to controls not exposed to HA. Of note, histochemical analyses of human prostatic tissues showed significantly higher expression of HA in BPH and prostate cancer stroma relative to stroma of normal prostate. Collectively, these results suggest that changes in ECM involving increased levels of HA contribute to the growth of prostatic epithelium with aging.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hyaluronan in aged collagen matrix increases prostate epithelial cell proliferation

Damodarasamy

Vernon

Chan

et al. 2014

In Vitro Cell.Dev.Biol.-Animal

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“…Besides its mitogenic effects, there is some evidence that PDGF-BB stimulates the biosynthesis of hyaluronan, one of the most studied and applied anti-adhesives (Meier and an important lubricant of the intrasynovial fluid in healthy tendons. Biosynthesis of hyaluronan, as a result of PDGF-BB application, has been reported for tenocytes (Thomopoulos et al, 2009), as well as for prostate smooth muscle cells (Pullen et al, 2001), cardiomyocytes (Hellman et al, 2010) and temporomandibular joint disc-derived cells (Hanaoka et al, 2006). The application at the wound site of a sustainable, releasing, PDGF-BB-loaded device, not only accelerated wound healing 1 and 2 weeks post-surgery , but also enhanced significantly the gliding of the tendons, as found 3 weeks post-intervention .…”

Section: Fibrin-based Delivery Devicementioning

confidence: 99%

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova

Buschmann

2017

eCM

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To promote and support tendon healing, one viable strategy is the use or administration of growth factors at the wound/rupture site. Platelet derived growth factor-BB (PDGF-BB), together with other growth factors, is secreted by platelets after injury. PDGF-BB promotes mitogenesis and angiogenesis, which could accelerate tendon healing. Therefore, in vitro studies with PDGF-BB have been performed to determine its effect on tenocytes and tenoblasts. Moreover, accurate and sophisticated drug delivery devices, aiming for a sustained release of PDGF-BB, have been developed, either by using heparin-binding and fibrin-based matrices or different electrospinning techniques.In this review, the structure and composition, as well as the healing process of tendons, are described. Part A deals with in vitro studies. They focus on the multiple effects evoked by PDGF-BB on the cellular level. Moreover, they address strategies for the sustained delivery of PDGF-BB. Part B focuses on animal models used to test different delivery strategies for PDGF-BB, in the context of tendon reconstruction. These studies showed that dosage and timing of PDGF-BB application are the most important factors for deciding which delivery device should be applied for a specific tendon laceration.

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“…Platelet-derived growth factor, in particular, has been shown to stimulate fibroblast proliferation and matrix and hyaluronan biosynthesis, 2,[5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] leading to an improved repair response. In the context of flexor tendon repair, increased matrix synthesis may lead to improved structural properties (eg, strength), and increased hyaluronan may lead to improved functional properties (eg, range of motion).…”

mentioning

confidence: 99%

The Early Effects of Sustained Platelet-Derived Growth Factor Administration on the Functional and Structural Properties of Repaired Intrasynovial Flexor Tendons: An In Vivo Biomechanic Study at 3 Weeks in Canines

Gelberman

Thomopoulos

Sakiyama‐Elbert

et al. 2007

The Journal of Hand Surgery

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Stimulation of Hyaluronan Synthetase by Platelet-Derived Growth Factor bb in Human Prostate Smooth Muscle Cells

Cited by 9 publications

References 22 publications

Hyaluronan in aged collagen matrix increases prostate epithelial cell proliferation

Hyaluronan in aged collagen matrix increases prostate epithelial cell proliferation

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

The Early Effects of Sustained Platelet-Derived Growth Factor Administration on the Functional and Structural Properties of Repaired Intrasynovial Flexor Tendons: An In Vivo Biomechanic Study at 3 Weeks in Canines

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