Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic hormone in sturgeons

Abstract

We showed that the percentage of sturgeon oocytes ovulating in vitro in Ringer solution modified for sturgeons (RMS) considerably depends on the concentration of sodium bicarbonate and the concentra tion of progesterone. Under optimal conditions (0.5 g/L of sodium bicarbonate and 30 ng/mL of progester one), it can be higher than 80. Oocytes that matured and ovulated under such conditions are capable of nor mal development. In the best case, approximately 70% of developing embryos (of the number of ovulated oocytes) reach the stage of hatching (dead line of observation). This method of producing offspring based on the insemination of oocytes that have matured and ovulated in vitro can be used in work with single females of rare and disappearing sturgeon species.

Seasonal changes in oocyte maturational competence, ovulatory competence and gonadotropin sensitivity of ovarian follicles in hybrid sturgeon, bester (Huso huso × Acipenser ruthenus )

“…Although the concentration we used (1 mg ml −1 ) may seem high, our results showed that this concentration was optimal. Skoblina and Goncharov () succeeded in inducing in vitro ovulation in A. ruthenus by using 0.2 mg ml −1 of aci‐GTH, a preparation of stellate sturgeon pituitary gonadotropic hormone. In vivo ovulation is induced by MIS, which is triggered by an LH surge.…”

Dynamics of the content of H2O, Na, K, Ca and Mg in the eggs of bream, Abramis brama L. in natural conditions and under stress

“…It is shown that the result depends on conditions of incubation and application of hormonal preparations [61,62]. It is required to search optimal methods to stimulate maturing of oocytes and ovulation process in industrial conditions.…”

“…Normal concentrations of 17,20β-dihydroxy-4-pregnen-3-one hormone, which promote final oocyte maturation rapidly increase just prior to ovulation and spawning, and remain elevated throughout the duration of spawning activity [58][59][60]. Watering of the oocytes of loach and sturgeon fishes was stimulated in vitro by combining influence of progesterone and chorial gonadotropin on follicles [61,62].…”

Effect of sodium orthovanadate on ovulation isolated ovarian follicles in Siberian Sturgeon (Acipenser baerii) In Vitro

“…Using a hormonal stimulation model in vitro of maturation and ovulation of sturgeon oocytes allows solving various fundamental and applied problems [3]. While the molecular mechanisms of maturation of fish oocytes were well understood [4,5], the ovulation process is still poorly understood.…”