Stimulation of plant growth and biocontrol by Bacillus amyloliquefaciens subsp. plantarum FZB42 engineered for improved action

Qiao, Junqing; Wu, Huijun; Huo, Rong; Gao, Xuewen; Borriss, Rainer

doi:10.1186/s40538-014-0012-2

Cited by 71 publications

(54 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This interpretation was also supported by the fact that the uptake of P from TSP-fertilised soil was not significantly different between BE treatments (Table 6a). The direct effects of these microbial inoculants on the plants are in line with earlier work showing that Pro and RhVi may elicit defence responses in plants [41,56], thus directly affecting the plant's metabolism. In the P0 treatment, a positive effect of Pro and RhVi was observed on the total P content of the aboveground biomass, which seemed to indicate that under these P-limited conditions the two BEs did improve plant P uptake, even though a BE-mediated increase in P H2O was not observed.…”

Section: Did the Added Bioeffectors Affect The Growth Of Plants And Psupporting

confidence: 83%

Effect of bioeffectors and recycled P-fertiliser products on the growth of spring wheat

Lekfeldt

Rex

Mercl

et al. 2016

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

Background:The recycling of waste products into P fertilisers in agriculture is advisable from the perspective of sustainability. Bioeffectors (BEs), which have the ability to increase the plant uptake of P from recycled fertiliser products, may increase the fertiliser value of these products. This paper investigated the effect of a range of different recycled fertilisers on the growth and P uptake of wheat in pot experiments conducted at three different locations in Europe. Furthermore, investigations were undertaken as to whether the addition of a range of bioeffectors could significantly enhance P availability, P uptake and plant growth.Results: BE additions were found not to significantly increase the aboveground biomass of wheat plants or the uptake of P when plants were fertilised with recycled fertiliser products. This was shown across a range of pot experiments with soils of different P status. Only in the case of the positive control P fertiliser (TSP) was a positive effect of Proradix and RhizoVital on plant growth observed in one of the experiments, while in the same experiment RhizoVital and Biological fertiliser DC had a negative impact on plant biomass when the P fertiliser was Thomas phosphate. With regard to P uptake, there was only a slight positive effect of Proradix in plants not supplied with P fertiliser in this experiment. Clear differences were seen in the efficiency of P fertilisers. Generally, sewage sludge ash performed quite poorly (20-40 % of TSP), while sewage sludge, Thomas phosphate, P-enriched slag and the fibre fraction of pig manure all had a high availability of P (>74 % relative to TSP). Compost composed mainly of garden/park waste and sewage sludge was intermediate in availability (40-70 %). The elemental composition of the harvested wheat plants was significantly affected in all cases by the different P fertilisers added. The BE treatments significantly affected the elemental composition of the aboveground biomass in one of the experiments where the product Proradix had the greatest effect on elemental composition. Conclusions:In conclusion, the experiments revealed a wide difference in the bioavailability of P in the different waste products, but the added microorganisms demonstrated a limited capacity to influence plant P uptake across a range of soils and waste products.

show abstract

Section: Did the Added Bioeffectors Affect The Growth Of Plants And Psupporting

confidence: 83%

Effect of bioeffectors and recycled P-fertiliser products on the growth of spring wheat

Lekfeldt

Rex

Mercl

et al. 2016

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

show abstract

“…Spray voltage was set at 4.5 kV for both modes, and capillary and heat block temperatures were set at 200 °C. An accumulation time of 20 ms and a mass range of 100-700 Dalton was set for both scans, resulting in a loop cycle of 1.80 s. Tandem MS scans were set with CID energy at 50 units, an accumulation time of 40 ms and a mass range of 80-700 for MS 2 and 60 ms and 50-500 for MS 3 , respectively. Ion precursor threshold limits used were, respectively, 300,000 for MS 2 and 50,000 for MS 3 .…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…In this light, a developing research issue is the use of microbial species, alone or in combination with agricultural and industrial waste byproducts as biostimulants of plant growth (bioeffectors). Microorganisms such as mycorrhizae [1] and, more recently, bacteria [2] have been proved to optimize plant nutrients intake, thereby replacing inorganic fertilization without exhausting N, S and P stocks in heavily exploited soils. It is believed that such species can promote plant development, increase yields and counter stress conditions by acting directly or indirectly on the physiology of plants and, hence, on the stimulation of metabolic pathways [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…This information, in turn may be correlated with changes in metabolism which the treated plants underwent when treated with different microbial biostimulants such as Trichoderma harzianum [11], Pseudomonas spp. [19], Bacillus amyloliquefaciens [2] and Pseudomonas jessenii [20] which are raising the interest of research due to their properties, and evaluate which metabolites were the results of the biostimulation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Nebbioso¹,

Martino

Eltlbany

et al. 2016

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

Background: In light of the growing interest for eco-compatible fertilization, tomato plant roots were treated with four different strains of microorganisms (B1-B4) capable of positively affecting plant growth. The methanolic extracts from treated roots were analysed by reverse phase ultra-high-performance liquid chromatography hyphenated with ion trap time-of-flight high-resolution mass spectrometry to compare their metabolites with that of control plants (B0). Results:We found, in both treated and control plants, several primary metabolites, such as fatty acids and coumaric acid, and other compounds associated with secondary metabolism pathways such as that of cyclopentaneoctanoic acid (CPOA) or hydroxyoctadecadienoic acid (HODE), and additional molecules which were not characterizable with the available data. A semiquantitative assessment of all metabolites became the basis for further processing the metabolic results by principal component analysis, which highlighted significant differences in the PC1 and PC2 components. The PC1 was particularly affected by the presence of arachidonic acid, myristic acid, and two unidentified metabolites. It effectively differentiated control plants from all bioeffectors treatments, and, in particular, the B4 treatment from the rest (B1-B3). The PC2 was mainly affected by palmitic acid, heptadecanoic acid, two CPOAs, one HODE and two unidentified metabolites. These metabolites successfully differentiated the B0 control from all the bioeffectors treatments, and, especially, showed a difference between B1 and B2. Conclusions:Our findings suggest that changes in secondary pathways of lipid metabolism may underlie the biostimulation exerted by the four microbial bioeffectors of this study, and that LC-MS coupled by multivariate analysis can easily fingerprint the metabolic alterations induced by bioeffectors in tomato roots.

show abstract

“…B. amyloliquefaciens GP4-1, GR2-2, GR4-5 iturin A surfactin , GR4-5 bacillomycin D . (Qiao , 2014). Bacillus spp.…”

Section: Ef1amentioning

confidence: 99%

Biological Control of Fusarium Stalk Rot of Maize Using Bacillus spp.

Han¹,

Park²,

Kim³

et al. 2015

Research in Plant Disease

View full text Add to dashboard Cite

Maize (Zea mays L.) is an economically important crop in worldwide. While the consumption of the maize is steadily increasing, the yield is decreasing due to continuous mono-cultivation and infection of soil-borne fungal pathogens such as Fusarium species. Recently, stalk rot disease in maize, caused by F. subglutinans and F. temperatum has been reported in Korea. In this study, we isolated bacterial isolates in rhizosphere soil of maize and subsequently tested for antagonistic activities against F. subglutinans and F. temperatum. A total of 1,357 bacterial strains were isolated from rhizosphere. Among them three bacterial isolates (GC02, GC07, GC08) were selected, based on antagonistic effects against Fusarium species. The isolates GC02 and GC07 were most efficient in inhibiting the mycelium growth of the pathogens. The three isolates GC02, GC07 and GC08 were identified as Bacillus methylotrophicus, B. amyloliquefaciens and B. thuringiensis using 16S rRNA sequence analysis, respectively. GC02 and GC07 bacterial suspensions were able to suppress over 80% conidial germination of the pathogens. GC02, GC07 and GC08 were capable of producing large quantities of protease enzymes, whereas the isolates GC07 and GC08 produced cellulase enzymes. The isolates GC02 and GC07 were more efficient in phosphate solubilization and siderophore production than GC08. Analysis of disease suppression revealed that GC07 was most effective in suppressing the disease development of stalk rot. It was also found that B. methylotrophicus GC02 and B. amyloliquefaciens GC07 have an ability to inhibit the growth of other plant pathogenic fungi. This study indicated B. methylotrophicus GC02 and B. amyloliquefaciens GC07 has potential for being used for the development of a biological control agent.

show abstract

Stimulation of plant growth and biocontrol by Bacillus amyloliquefaciens subsp. plantarum FZB42 engineered for improved action

Cited by 71 publications

References 40 publications

Effect of bioeffectors and recycled P-fertiliser products on the growth of spring wheat

Effect of bioeffectors and recycled P-fertiliser products on the growth of spring wheat

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Biological Control of Fusarium Stalk Rot of Maize Using Bacillus spp.

Contact Info

Product

Resources

About