Stimulation of the initiation of DNA synthesis and cell division in several cultured mouse cell types

O'Farrell, M.; Clingan, Dorothie; Rudland, Philip S.; Asúa, Luis Jiménez de

doi:10.1016/0014-4827(79)90156-3

Cited by 26 publications

(13 citation statements)

References 20 publications

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“…Investigators have shown that B12 is required for the optimum growth of certain cell types in serum-free medium (15, 23, 24, 26, 27 and 28). O'Farrel et al (26) reported that 3T3 cells have a dual requirement for B12 and Hyp, whereas 3T6 cells require B12 alone. The folate or folinate requirement is related to the concentrations of adenine, dThd and glycine (22).…”

Section: Discussionmentioning

confidence: 99%

Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

Matsuya

Yamane

1986

Cell Struct. Funct.

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Section: Discussionmentioning

confidence: 99%

Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

Matsuya

Yamane

1986

Cell Struct. Funct.

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“…Radioactive labeling of cultures and sample preparation At the appropriate time the medium was removed, the cell monolayers were washed twice with phosphate-bufTered saline, C a f 2 and Mgf2-free (PBS) and the medium was replaced with 5 ml of methionine-free DEM (purchased from Flow Laboratories, Irvine, Ayrshire, Scotland), 5% DEM, 5% dialysed FCS (against PBS: O'Farrell et al, 1979), hormones and inducers as in the previous 24 hr, and 10 pCi/ml of L-[35S]methionine (Amersham International, U.K.). The cultures were usually labeled for 24 hr unless otherwise stated, since this represented the steady state labeling time (Rudland, 1974).…”

Section: Methodsmentioning

confidence: 99%

Identification of novel, stage‐specific polypeptides associated with the differentiation of mammary epithelial stem cells to alveolar‐like cells in culture

Paterson

Rudland

1985

Journal Cellular Physiology

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A linear pathway of morphologically intermediate cells has been identified between the cuboidal epithelial stem cells and the doming alveolar-like cultures of the cell line Rat Mammary (Rama) 25 in the order: cuboidal----grey----dark----dark droplet cell----doming cultures. The overall process can be accelerated by dimethyl sulfoxide (DMSO) or retinoic acid (RA) in the presence of mammotrophic hormones. From 400-450 [35S]methionine-labeled polypeptides that are routinely separated by two-dimensional gel electrophoresis approximately only 3% change during this process. As the Rama 25 cultures become confluent, three polypeptides of molecular weights (MW) 35 kD (pl = 7.7), 45 kD (pl = 7.5) and 33 kD (pl = 7.7) increase dramatically in radioactive abundance. These increases correspond to increases in numbers of grey cells for the 35 kD polypeptide, to increases in numbers of dark cells together with increases in peanut lectin-binding-ability for the 45 kD polypeptide, and to increases in the numbers of dark cells and in the numbers of droplet cells for the 33 kD polypeptide. After treatment with DMSO, RA or in spontaneously doming cultures, a second set of four polypeptides of MW 26 kD (pl = 5.9), 27 kD (pl = 6.2), 30 kD (pl = 7.2), and the same 33 kD polypeptide as above increase with the increase in numbers of droplet cells, domes, and increase in casein secretion. A variant of Rama 25, Rama 259, which fails to produce droplet cells, domes, or to secrete casein with DMSO and hormones also shows the same changes in the first set but not in the second set of polypeptides. The elongated, myoepithelial-like cell line derived from Rama 25, Rama 29, which cannot undergo any of the above intercellular conversions, fails to show changes in any of these polypeptides. Major changes in radioactive polypeptides have been confirmed for nonradioactive polypeptides and for polypeptides labeled for 4 hr with [35S]methionine. The synthesis of these novel polypeptides thus marks specific morphological stages of the differentiation of mammary epithelial stem to alveolar-like cells in culture, and as such may mark similar differentiation stages in vivo.

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“…Prostaglandins are involved in the regulation of DNA synthesis, and in cell proliferation and differentiation in different tissues (Taylor & Polgar, 1977;O'Farrell, Clingan, Rudland & Jimenez de Asua, 1979;Jimenez de Asua, Otto, Lindgren & Hammastrom, 1983). The effect may be stimulatory (Andreis, Whitfield & Armato, 1981;Otto, Nilsen-Hamilton, Bossera/.…”

Section: Introductionmentioning

confidence: 97%

Indomethacin inhibits the effects of oestrogen in the anterior pituitary gland of the rat

Ga²,

Ac³

et al. 1989

Journal of Endocrinology

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Two inhibitors of prostaglandin synthesis, indomethacin and aspirin, blocked the increase of oestrogen-binding sites in the nuclear subcellular fraction, an increase which occurs after the administration of oestradiol. Consequently the biological effects of oestrogens in the anterior pituitary gland of the rat (prolactin synthesis, concentration of progesterone-binding sites and cell proliferation) are diminished. The anterior pituitary gland synthesized prostaglandin F2 alpha (PGF2 alpha), PGE2 and PGD2 from arachidonic acid. This synthesis was blocked when indomethacin was added to the culture media. Oestrogen increased the concentration of PGE2: an increase that was partially prevented by indomethacin. Prostaglandins may have an important role on the effects of oestrogen in the anterior pituitary gland of the rat.

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Stimulation of the initiation of DNA synthesis and cell division in several cultured mouse cell types

Cited by 26 publications

References 20 publications

Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

Identification of novel, stage‐specific polypeptides associated with the differentiation of mammary epithelial stem cells to alveolar‐like cells in culture

Indomethacin inhibits the effects of oestrogen in the anterior pituitary gland of the rat

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