Stimulation of wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF)

Abstract

IntroductionThe objective of this study is to stimulate wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF).Materials and methodsInspired by the crosslinking mechanism in algae-based adhesives, hydrogels were fabricated with gum arabic, pectin, and Ca2+. The physical properties of the bioinspired hydrogels were characterized, and the in vitro release of bFGF was investigated. Then, the in vitro scratch assay for wound healing and in vivo wound healing experiment in a full-thickness excision wound model were performed for the bioinspired hydrogels with bFGF. Finally, histological examinations and organ toxicity tests were conducted to investigate the wound healing applications of the bioinspired hydrogels with bFGF.ResultsThe in vitro and in vivo results showed that the bioinspired hydrogels with bFGF could significantly enhance cell proliferation, wound re-epithelialization, collagen deposition, and contraction without any noticeable toxicity and inflammation compared with the hydrogels without bFGF and commercial wound healing products.ConclusionThese results suggest the potential application of bioinspired hydrogels with bFGF for wound healing.

A novel dual-adhesive and bioactive hydrogel activated by bioglass for wound healing

“…A bioactive hydrogel may be able to stimulate cell migration 18 , proliferation 19 , and differentiation 20 , and enhance blood vessel formation during the woundhealing process 21 , so that the hydrogel can significantly reduce the healing time [21][22][23] . Currently, hydrogels with bioactivity are mainly obtained by the addition of growth factors 24 , which still faces challenges in clinical applications, such as their controlled delivery and activity maintenance.…”

Palatal Mukozal Hasar Oluşturulan Tavşanlarda Profilaktik Hypericum Perforatum Yağ Uygulamasının Antioksidan Özellikleri

“…Fibroblast growth factor (FGF)-2 and vascular endothelial growth factor (VEGF) have been determined to promote angiogenesis synergistically, and therefore, have much caution in the field of wound healing [27]. It has been reported that topical administration of FGF-2 could enhance cell proliferation and migration, wound reepithelialization and collagen deposition [28,29]. In an animal model of surgically wounded submandibular glands, the use of collagen gels with FGF-2 improved salivary gland regeneration [30].…”

Stammzellen in der Regenerativen Medizin – Translationale Hürden und Möglichkeiten zur Überwindung

“…Bis heute ist also die Effizienz von B-FGF bezüglich der Wundheilung unklar -weitere Studien mit einer größeren Patientenzahl fehlen. Zusätzlich können spezielle Hydrogels oder Co-Faktoren die Effizienz von B-FGF signifikant steigern [69,70].…”