Stimulatory Effect of Polynucleotides on Short Term Leukocyte Cultures

Friedman, Harold M.; Johnson, Arthur G.; Pan, Pauline Lining

doi:10.3181/00379727-132-34336

Cited by 20 publications

(4 citation statements)

References 3 publications

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“…However, NTx mice which received poly A:U were able to reject grafts at the same rate as unoperated mice. Thus, cellular as well as humoral immunity appeared to be restored by poly A:U in NTx mice, corroborating earlier studies on the effect of poly A:U on cellmediated immune reactions (16). by Poly A : U.…”

Section: Restoration Of the Immune Response To Srbc In Ntx Mice By Thsupporting

confidence: 89%

Regulation of the Immune System by Synthetic Polynucleotides

Cone

Johnson

1971

The Journal of Experimental Medicine

100

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Polyadenylic-polyuridylic acid complexes, a potent adjuvant to the immune response, were tested for action on thymic-influenced and bone marrow-derived lymphocytes in model systems deficient in one or the other of these cells. Adult mice, thymectomized at birth or mice treated with heterologous antithymocyte serum produced 90–95% fewer splenic rosette-forming cells than normal mice in response to an injection of sheep erythrocytes. Intravenous injection of complexes of homoribopolynucleotides, polyadenylic and polyuridylic acids, poly A:U with SRBC restored immunologic competence to NTx- or ATS-treated mice such that they produced normal or near normal levels of splenic RFC. In addition, injection of poly A:U enabled NTx mice to reject allogeneic skin grafts at the same rate as control mice with an intact thymus. Further reduction in residual thymocytes by combining neonatal thymectomy with ATS treatment reduced the number of anti-SRBC RFC induced by poly A:U. Lethally irradiated mice which received SRBC, excess bone marrow cells, and as few as 40,000 thymic lymphocytes were stimulated by poly A:U to produce RFC. No adjuvant effect was observed when irradiated mice received excess thymic lymphocytes and low doses of bone marrow cells with poly A:U. The results suggested that the adjuvant action of poly A:U was exerted on the thymic-influenced, antigen-reactive cell and that restoration of immunocompetence to NTx- or ATS-treated mice was caused by amplification of a small number of residual antigen-reactive cells which were influenced by the thymus in utero before thymectomy, or which survived treatment with ATS.

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Section: Restoration Of the Immune Response To Srbc In Ntx Mice By Thsupporting

confidence: 89%

Regulation of the Immune System by Synthetic Polynucleotides

Cone

Johnson

1971

The Journal of Experimental Medicine

100

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“…Allison has indicated that macrophages are necessary in the poly(A) -poly(U) augmentation of immune responses (antibody synthesis) in mixed lymphocyte cultures and that elimination ofT cells through the action of anti-B serum and complement results in an inhibition of overall thymidine incorporation (24). Friedman et al (402) have found that poly(A) * poly(U) increased the incorporation of thymidine in lymphocytes from tuberculin-positive subjects when those lymphocytes were exposed in vitro to tuberculin.…”

Section: Delayed Hypersensitivity and Cell-mediatedmentioning

confidence: 99%

Mycobacterium

Barksdale¹,

Kim²

1977

Bacteriol Rev

135

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M. xenopi ( 1037) M. paraffinicum (292) M. kansasii (198; 500) M. scrofulaceum (940; 941) 1950 M. terrae (978; 1225)c M. intracellulare (275; 996)d M. ukerans (746; 370) M. fortuitum (276, see also reference 519) M. microti (1246; 963)e M. thamnopheos (53)' M. marinum (52) M. chelonei (403; 126),g M. lepraemurium (1094; 769) M. phlei (842; 713)h M. bovis (1070; 585) M. paratuberculosis (552, 1188; 126)Y M. avium (1104, 754; 244, see also [717]) M. smegmatis (30; 713) M. tuberculosis (623; 712) M. keprae (species forma, 489, 712)J 220 BARKSDALE AND KIM BACTERIOL. REV.

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“…Their experiments were preceded by the demonstration by Friedman et al [27] that poly A : U enhanced remarkably the uptake of tritiated thymidine in human mixed lymphocyte cultures or cells sensitized and re-exposed to diphtheria toxoid. In both instances, the enhancing effects required antigen mediation and were specific, and together with the studies of Mardiney et al [31,32] emphasize the ability of poly A : U to enhance normally subliminal doses of antigen in the mixed leucocyte reaction (MLR).…”

Section: T Cellmentioning

confidence: 99%

Modulation of the immune system by synthetic polynucleotides

Johnson

1979

Springer Semin Immunopathol

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Stimulatory Effect of Polynucleotides on Short Term Leukocyte Cultures

Cited by 20 publications

References 3 publications

Regulation of the Immune System by Synthetic Polynucleotides

Regulation of the Immune System by Synthetic Polynucleotides

Mycobacterium

Modulation of the immune system by synthetic polynucleotides

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